Quantitative Monitoring of the Fermentation Process of a Barley Malt Mash by Benchtop 1H NMR Spectroscopy

In order to investigate benchtop NMR spectroscopy as a monitoring tool for fermentation processes, we used a barley malt mash and took various samples over time and analysed them by NMR spectroscopy with 3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid sodium salt (TSP-d4) as an internal standard for the quantification of ethanol and validated the results by two different enzymatic standard test kits for ethanol analysis. We could show that the results between NMR spectroscopy and test kits were consistent with NMR having a much lower standard deviation. Finally, we discussed the applicability of the method as well as the possibility to quantify various other substances.


Introduction
The fermentation of natural products for the production of alcoholic drinks and spirits has a history dating back in time as long as the history of humankind itself (Gately 2009;Hames 2014;Phillips 2014). And though the experience of the manufacturer has an important influence on the results of the process, modern methods for the monitoring of the fermentation and for the quality control of the final product are essential for the commercial success of the product. Various substances like ethanol and carbohydrates need to be quantified or, in the case of toxic substances like methanol, acetaldehyde, and ethyl acetate, excluded or quantified in relation to critical values.
In the field of food analytics, we find various methodologies for the determination of alcohol and volatile by-products in alcoholic drinks and spirits. There are official methods which are defined by corresponding regulations ("COMMISSION REGULATION (EC) No 2870/2000 of 19 December 2000 laying down Community reference methods for the analysis of spirits drinks," 2000) which include standard methods like pycnometry, electronic and hydrostatic balance based densimetry, and distillative methods and for which different standard operating procedure collections are available (Schmitt 1983;Tanner and Brunner 1987).
But also modern, mainly instrumentally based, methodologies have been developed by various groups over the past two decades, including near-infrared derivative spectrometry (Gallignani et al. 1993), high-performance liquid chromatography (Yarita et al. 2002), gas chromatography (Wang et al. 2003, and high-field 1 H NMR spectroscopy (Kew et al. 2017;Zuriarrain et al. 2015). The last one is a powerful tool, which allows qualitative and quantitative analyses of various substances like the ones involved in the fermentation process mentioned above, though in the case of mixtures the quantification can be challenging. Nonetheless, 1 H NMR has become an interesting analytical method used in various scientific fields beyond structural chemical analysis such as determination of blood alcohol concentration (Zailer and Diehl 2016) and quantitative metabolomics and metabolic profiling (Larive et al. 2015) and of course in food sciences (Hatzakis 2019).
One great advantage of magnetic resonance spectroscopy in the field of quantitative analysis is the fastness of the method. Once a standard operating procedure has been established, the analysis itself can be performed in under 15 min including sample preparation, measurement, and evaluation. On the other hand, a great disadvantage of high-field NMR is the high costs for the acquisition of the instrument as well as the following maintenance costs including personal, liquid nitrogen (weekly), and liquid helium (twice the year for modern magnets) for the cooling of the superconducting magnet.
Coming more from the field of teaching, the lowfield benchtop NMR technology started establishing itself in the field of scientific research over the past 5 years thanks to various meanwhile available machines on the market with 1 H frequencies ranging from 43 up to 100 MHz. These modern machines work with permanent magnets which are practically maintenance free and are delivered with a solid working basic software allowing to perform various 1D and 2D methods (if a second nuclei such as 13 C is available) (Blümich 2019;Blümich and Singh 2018;Lawson et al. 2020).
Various publications of the past few years show the growing importance and indicate clearly how benchtop NMR spectroscopy establishes itself in interdisciplinary scientific research (Antonides et al. 2019;Gunning et al. 2018;Jakes et al. 2015).

Material and Methods
Chemicals and reagents were purchased from Carl Roth and were used without further purification. For the determination of ethanol, two different test kits have been used, the "Enzytec Liquid Ethanol" from R-Biopharm-an NAD + /alcohol dehydrogenase oxidation based method where the absorption of NADH is used to determine the ethanol concentration-and the "Ethanol UV Test" from R-Biopharm and Roche Diagnostics, which uses an additional oxidation step with aldehyde dehydrogenase to oxidize acetaldehyde to acetic acid. Both test kits work with the photometric determination of NADH.

NMR Sample Preparation and General Procedure for NMR Measurements
For sample preparation, 10-ml samples of the fermentation mixture have been centrifuged yielding in a clear solution, which has been heated to 80°C in a sealed tube for 15 min to stop enzymatic activities. After cooling to room temperature, the tube was shaken for 60 s to homogenize the solution. 100 μl of this solution and 400 μl of the TSP-d 4 D 2 O solution have been mixed and placed in a 5-mm standard NMR tube resulting in a TSP-d 4 standard concentration of 40 mM, which has been used to determine the concentrations of unknown analyte in solution.
1 H NMR spectra were recorded on a Magritek Spinsolve 60 Carbon Benchtop NMR spectrometer. Chemical shifts are reported in ppm relative to solvent signal (HDO: δH = 4.79 ppm). The spectra were recorded at a temperature of 25°C with the 1D Proton standard protocol using 4 scans with a 90°excitation pulse (7 μs) covering a spectral range from 46 to −37 ppm (Magritek Spinsolve standard NMR conditions). 32k data points are acquired with an acquisition time of 6.5 s and a repetition time (recycle delay) of 15 s. Data processing has been performed with MestReNova version 14.1.0 including zeroth and first-order phase correction and baseline correction with a Bernstein polynomial fit of third order.

Test Kit Preparation and General Procedures
The test kit preparation follows the general instructions from R-Biopharm and Roche Diagnostics available on the website of R-Biopharm (https://food.r-biopharm.com).
The preliminary preparation of the fermentation mixture follows the procedure described above (NMR sample preparation). Sample solutions with potentially higher concentrations have been diluted with deionized water. UV spectra have been recorded at 340 nm with a Shimadzu UV Visible Spectrophotometer UVmini-1240.

Origin of the Barley Malt Mash and Sampling
The wort-the liquid extracted from the mashing processhas been provided by the Badische Staatsbrauerei Rothaus AG. Commercially available Saccharomyces cerevisiae has been used for the fermentation process, from which 10 samples have been retrieved over a period of 4 days, after which the fermentation was completed.

Results and Discussion
Before the start of the study, NMR spectra of pure glucose, saccharose, maltose, and ethanol have been measured under the standard procedure mentioned above to obtain spectral data for comparison. For both glucose and maltose, an anomeric mixture of alpha:beta = 36:64 could be found in equilibrium determined by integrating the anomeric doublets. Figure 1 shows the NMR spectrum of the start of the fermentation process. Two doublets can be seen at 5.43 and 5.25 ppm, the first belonging to glycosidic anomeric protons of mainly maltose and also the glucosidic anomeric proton of saccharose which lies beneath this doublet. Together with the soluble dextrinic structures also present in the sample, the quantification of maltose becomes practically impossible.
The second singlet at 5.25 ppm belongs to the alpha protons of glucose and of the reducing end of maltose, making it theoretically possible to determine the concentration of glucose and maltose in sum. Nonetheless, we decided to put this possibility aside because with proceeding fermentation, the concentration drops fast and the S/N ratio declines rapidly under our standard procedure conditions resulting in unreliable results. Finally, no triplet can be seen at 1.18 showing that no ethanol has been produced so far. Figure 2 shows the end of the fermentation process. Herein, only traces of remaining carbohydrates can be seen and a high amount of ethanol is detected without seeing relevant amounts of impurities like ethyl acetate or acetaldehyde. To calculate the concentration of ethanol in the sample, we calibrated the integral of the TSP-d 4 signal at approx. 0 ppm to 9, due to the nine protons of the trimethylsilyl group of the molecule.
After integrating the triplet at 1.18 ppm, which belongs to the methyl group of ethanol, the molar concentration can be determined by using following formula: Int met stands for the integration value of the triplet signal at 1.18 ppm, the TSP-d 4 concentration in our case was adjusted to 40 mM, and the dilution factor is 5/1. In the case of the NMR measurement shown in Fig. 2 Figure 3 shows the process of ethanol production in the barley malt mash graphically over time. Finally, Fig. 4 depicts the progress of ethanol production via a stacked NMR graphic showing nicely how the ethanol concentration increases, while the carbohydrate signals vanish from the spectra over time.
As displayed in Table 1 and in Fig. 3, the results of the test kits and from the NMR experiments match well. Interestingly, Fig. 1 Spectrum of the start of the fermentation process both the NMR and the Enzytec Liquid Ethanol kit show a slight decrease in ethanol production between the hours 25 and 40, though the effect is less distinct with the test kit.
More important, differences in the standard deviation (SD) can be observed for the different methods. While the NMR measurements show reproducible values resulting in a low SD, the Enzytec Liquid Ethanol test kit results in a broader distribution producing higher SD values and therefore a lower precision. This can partially be explained by the limitations of the enzymatic test kits. While it is theoretically and practically possible to determine every possible mixing ratio of ethanol and water by NMR spectroscopy, the measurement range for the enzymatic test kits given by the manufacturer's instructions lies between 20 and 300 mg/l. This means that additional dilution steps are potentially necessary to get the samples into the recommended range, which leads to a second disadvantage of the test kits compared to the NMR spectroscopy: the sample preparation, which is in case of the NMR spectroscopy much faster and easier resulting in a lower chance for operational errors.  On the first sight, the comparison of costs for the different methods is not easy. Though benchtop NMR spectrometers are much cheaper than high-field spectrometers and have most notably practically no maintenance costs, it is still a noteworthy value necessary for purchasing such a device. In light of this, an NMR spectrometer is used for a variety of applications from the determination of chemical structures of small molecules up to reaction kinetics. Furthermore, the timesaving workflow combined with the low follow-up costs (Magritek benchtop spectrometers do not necessarily need deuterated solvents due to an internal deuterium lock standard and NMR tubes can be used multiple times) must be considered an advantage. Based on this, the use of NMR spectroscopy for the presented purpose will over time be a time-and cost-saving alternative to enzymatic test kits. An economic quantification of this advantage is, nevertheless, not easy and depends significantly on the amount of measurements over time.
In terms of the quantification of the carbohydrates with NMR, as mentioned above, more work and method development is necessary to overcome some present limitations. One possibility would be to freeze dry the samples to get rid of volatile components and water. Then, NMR spectroscopy, i.e. with DMSO-d6 as solvent, could provide more and especially quantifiable information.

Conclusion
In this work, we were able to validate benchtop NMR spectroscopy as a method for the quantification of ethanol in a malt mash und compared the results with those of enzymatic test kits in terms of time, costs, applicability and accuracy. We could show that the results were at least comparable or better for the benchtop NMR-based quantification. Finally, we discussed the possibility to quantify other substances of the mash such as carbohydrates but had to admit that more research and work are required, to get reliable results.
Funding Open Access funding enabled and organized by Projekt DEAL. The acquisition of the benchtop NMR spectrometer has been financially supported by the "Fonds der chemischen Industrie", part of the "Verband der chemischen Industrie", as a new tool for teaching and research.

Declarations
Ethical Approval This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of Interest Pia Burkhardtsmaier declares that she has no conflict of interest. Kristina Pavlovskaja declares that she has no conflict of interest. Dennis Maier declares that he has no conflict of interest.
Stephanie Schäfer declares that she has no conflict of interest. Ulrike Salat declares that she has no conflict of interest. Magnus S. Schmidt declares that he has no conflict of interest.

Informed Consent Informed consent not applicable
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