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New Hapten Synthesis, Antibody Production, and Indirect Competitive Enzyme-Linked Immnunosorbent Assay for Amantadine in Chicken Muscle

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Abstract

Two haptens were synthesized by introducing spacer arms of different lengths and structures on the free amino of amantadine (AMA). 4-(chlorosulfonyl) benzoic acid (CSBA) was reacted with AMA to produce hapten AMA-CSBA, and succinic anhydride was reacted with AMA to produce hapten AMA-HS. And then, the two haptens coupled with bovine serum albumin (BSA) were prepared as immunogens for the production of monoclonal antibody (mAb). High-sensitive mAbs were obtained and characterized by using indirect competitive enzyme-linked immnunosorbent assay (icELISA). The best antibody was obtained in a heterologous icELISA with an IC50 value of 0.84 and 0.41 ng/mL for AMA and rimantadine, respectively, and negligible cross-reactivity (CR) with memantine and ribavirin. An icELISA was developed to detect AMA residue in chicken muscle. The limit of detection (LOD) was 1 μg/kg in spiked chicken muscle and the recoveries ranged from 93.6 to 116.9% with the coefficient of variations (CVs) below 9.5%. Good correlation between icELISA and HPLC-MS/MS results in the incurred tissues demonstrated the reliability of the developed icELISA. These results indicated that the new antibody was sufficiently sensitive and specific for the detection of AMA in real samples.

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Authors and Affiliations

  1. College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People’s Republic of China

    Zhaopeng Wang, Kai Wen, Xiya Zhang, Zhanhui Wang, Jianzhong Shen & Shuangyang Ding

  2. College of Food Science, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Xiangmei Li

Authors
  1. Zhaopeng Wang
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  2. Kai Wen
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  3. Xiya Zhang
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  4. Xiangmei Li
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  5. Zhanhui Wang
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  6. Jianzhong Shen
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  7. Shuangyang Ding
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Corresponding author

Correspondence to Shuangyang Ding.

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Funding

We acknowledge the financial support of the National Natural Science Foundation of China (no. 31472236).

Conflict of Interest

Zhaopeng Wang has no competing financial interests. Kai Wen has no competing financial interests. Xiya Zhang has no competing financial interests. Xiangmei Li has no competing financial interests. Zhanhui Wang has no competing financial interests. Jianzhong Shen has no competing financial interests. Shuangyang Ding has no competing financial interests.

Ethical Approval

We declare that the animal test in this study complies with the guidelines for the care and use of laboratory animals as described by the US National Institutes of Health. This article does not contain any studies with humans.

Informed Consent

All the authors named in the manuscript are entitled to the authorship and have approved the final version of the submitted manuscript.

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Wang, Z., Wen, K., Zhang, X. et al. New Hapten Synthesis, Antibody Production, and Indirect Competitive Enzyme-Linked Immnunosorbent Assay for Amantadine in Chicken Muscle. Food Anal. Methods 11, 302–308 (2018). https://doi.org/10.1007/s12161-017-1000-5

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  • DOI: https://doi.org/10.1007/s12161-017-1000-5

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