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BrcuHAC1 is a histone acetyltransferase that affects bolting development in Chinese flowering cabbage

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Abstract

Histone acetylation is an important posttranslational modification associated with gene activation. In Arabidopsis, histone acetyltransferase 1 (HAC1) can promote flowering by regulating the transcription of FLOWERING LOCUS C (FLC), a major floral repressor. The size of the full-length cDNA and genomic DNA sequences of the histone acetyltransferase 1 gene (BrcuHAC1) in Chinese flowering cabbage (Brassica rapa syn. campestris ssp. chinensis var. utilis) were 5846 bp and 7376 bp, with an open reading frame (ORF) coding for a peptide with 1689 amino acids. The expression levels of BrcuHAC1 in different tissues and different developmental stages were as follows: flower > leaf > stem > root, and completed bolting and flowering stage > 5th true leaf-stage > 4th true leaf-stage > 3rd true leaf-stage > 2nd true leaf-stage > 1st true leaf-stage. Silencing of BrcuHAC1 resulted in slow growth, and delayed bolting and flowering time in Chinese flowering cabbage. Molecular analysis showed that the mRNA level of FLC was increased, indicating that the delayed flowering phenomenon was mediated by FLC in the silenced group. In contrast, the expression levels of the autonomous-pathway genes were not significantly affected in the silenced group. In addition, the histone modification of FLC chromatin was also not affected in the silenced group. FLC is not the direct target gene of BrcuHAC1. However, BrcuHAC1 may affect the bolting and flowering time of Chinese flowering cabbage through the epigenetic modification of upstream factors of FLC.

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Acknowledgement

This research was funded by the National Natural Science Foundation of China (grant no. 31860560 and 31360484).

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Author notes

  1. XXF and ZM designed the research. SSC, ZM and HYF carried out the experiments. XXF, YYX and WCJ analysed the data. XXF, SSC and LS wrote the manuscript.

Authors and Affiliations

  1. College of Agronomy, Jiangxi Agricultural University, Nanchang, 330 045, People’s Republic of China

    Shucheng Si, Yifan Hu, Caijun Wu, Youxin Yang, Sha Luo & Xufeng Xiao

  2. Department of Animal Science, Jiangxi Biotech Vocational College, Nanchang, 330 200, People’s Republic of China

    Ming Zhang

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  1. Shucheng Si
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Correspondence to Xufeng Xiao.

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Corresponding editor: Manoj Prasad

Shucheng Si and Ming Zhang contributed equally to this work.

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Si, S., Zhang, M., Hu, Y. et al. BrcuHAC1 is a histone acetyltransferase that affects bolting development in Chinese flowering cabbage. J Genet 100, 56 (2021). https://doi.org/10.1007/s12041-021-01303-4

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  • DOI: https://doi.org/10.1007/s12041-021-01303-4

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