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Circadian Regulation of the Lactate Metabolic Kinetics in Mice Using the [1H-13C]-NMR Technique

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Abstract

Lactate is not only the energy substrate of neural cells, but also an important signal molecule in brain. In modern societies, disturbed circadian rhythms pose a global challenge. Therefore, exploring the influence of circadian period on lactate and its metabolic kinetics is essential for the advancement of neuroscientific research. In the present study, the different groups of mice (L: 8:00 a.m.; D: 20:00 p.m.; SD: 20:00 p.m. with 12 h acute sleep deprivation) were infused with [3-13C] lactate through the lateral tail vein for a duration of 2 min. After 30-min lactate metabolism, the animals were euthanized and the tissues of brain and liver were obtained and extracted, and then, the [1H-13C] NMR technology was employed to investigate the kinetic information of lactate metabolism in different brain regions and liver to detect the enrichment of various metabolic kinetic information. Results revealed the fluctuating lactate concentrations in the brain throughout the day, with lower levels during light periods and higher levels during dark periods. Most metabolites displayed strong sensitivity to circadian rhythm, exhibiting significant day-night variations. Conversely, only a few metabolites showed changes after acute sleep deprivation, primarily in the temporal brain region. Interestingly, in contrast to brain lactate metabolism, liver lactate metabolism exhibited a significant increase following acute sleep deprivation. This study explored the kinetics of lactate metabolism, hinted at potential clinical implications for disorders involving circadian rhythm disturbances, and providing a new research basis for clinical exploration of brain and liver lactate metabolism.

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Data Availability

All raw data and materials during the current study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by grants from the National Natural Science Foundation of China (82172160, 81970722, 82001119, 31970973, 82270861, 82271430, and 32271148).

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  1. Lili Chen and Kefan Wu have the same contribution to this work.

Authors and Affiliations

  1. Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, People’s Republic of China

    Lili Chen, Kefan Wu, Jiabao Hou, Yuan Zhang, Lian Liu & Zhongyuan Xia

  2. Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics, Wuhan, Hubei, 430071, People’s Republic of China

    Jingang He

  3. Songjiang Hospital and Songjiang Research Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, 201600, People’s Republic of China

    Jie Wang

  4. Institute of Neuroscience and Brain Diseases; Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, 441021, People’s Republic of China

    Jie Wang

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Contributions

Lili Chen, Kefan Wu, Jie Wang, and Zhongyuan Xia designed the study. Lili Chen, Kefan Wu, Jiabao Hou, Lian Liu, and Yuan Zhang performed the experiments. Lili Chen, Kefan Wu, and Jingang He contributed to the data. Lili Chen and Kefan Wu wrote the manuscript. Jie Wang and Zhongyuan Xia edited the article. The content of this manuscript has been reviewed, read, and agreed upon by all the designated authors.

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Correspondence to Jie Wang or Zhongyuan Xia.

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Chen, L., Wu, K., He, J. et al. Circadian Regulation of the Lactate Metabolic Kinetics in Mice Using the [1H-13C]-NMR Technique. Mol Neurobiol (2024). https://doi.org/10.1007/s12035-024-03927-w

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