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Upregulation of CELSR1 expression promotes ovarian cancer cell proliferation, migration, and invasion

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Abstract

Cadherin epidermal growth factor and laminin-G seven-pass G-type receptor 1 (CELSR1) is a planar cell polarity protein involved in the transmission of directional cues to align either individual cells within an epithelial sheet or multicellular clusters. CELSR1 has been suggested to play a role in glioma, breast cancer, and chronic lymphocytic leukemia development; however, whether it has a role in the pathogenesis of ovarian cancer remains unknown. The aim of this study was to determine the role of CELSR1 in ovarian cancer and elucidate its underlying molecular mechanisms. By analyzing gene expression data downloaded from the Cancer Genome Atlas database, we found that CELSR1 expression was upregulated in ovarian cancer tissues compared to that in normal ovarian tissues. High CELSR1 expression levels were associated with poor prognosis in patients with ovarian cancer. Cell proliferation, scratch, and transwell assays revealed that CELSR1 promoted the proliferation, migration, and invasion of ovarian cancer cells in vitro. In addition, transcriptome sequencing analysis revealed that CELSR1 knockdown in T29H cells resulted in the dysregulation of the expression of 1320 genes. Further analysis revealed that genes involved in proliferation- and migration-associated signaling pathways were regulated by CELSR1. Our study demonstrates that CELSR1 is highly expressed in ovarian cancer cells and regulates their proliferation and migration, suggesting its potential as a diagnostic marker and therapeutic target.

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Acknowledgements

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Funding

This work was supported by the National Natural Science Foundation of China (Grant numbers 81972648, 82172915).

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Authors and Affiliations

  1. Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015, Zhejiang, People’s Republic of China

    Jiwei Zuo, Anqi Zheng, Xingyue Wang, Zhicheng Luo, Yueming Chen, Xiaoxiao Cheng, Yuemei Zhao & Kai-Fu Tang

  2. Department of Radiation Oncology, Chongqing University Cancer Hospital, 181, Hanyu Road, Shapingba District, Chongqing, 400030, People’s Republic of China

    Xian Zhou

  3. Key Laboratory of Molecular Biology On Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Kai-Fu Tang

  4. Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Xing Du

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  1. Jiwei Zuo
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  2. Anqi Zheng
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  3. Xingyue Wang
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  4. Zhicheng Luo
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  8. Xian Zhou
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  9. Kai-Fu Tang
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  10. Xing Du
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Contributions

All authors contributed to the study conception and design. K-FT and XD conceived and devised the study. JZ, AZ, XW, ZL, YC, XC, YZ, and XZ performed the experiments and analyzed the data. JZ drafted the manuscript. K-FT and XD reviewed the manuscript. K-FT and XD supervised the research. All authors read and approved the final manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Kai-Fu Tang or Xing Du.

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Zuo, J., Zheng, A., Wang, X. et al. Upregulation of CELSR1 expression promotes ovarian cancer cell proliferation, migration, and invasion. Med Oncol 41, 10 (2024). https://doi.org/10.1007/s12032-023-02232-1

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