Geraniol Ameliorates the Motor Behavior and Neurotrophic Factors Inadequacy in MPTP-Induced Mice Model of Parkinson’s Disease

Many experiments affirm the notion that augmentation of neurotrophic factors (NTFs) activity, especially brain-derived neurotrophic factors and glial cell-derived neurotrophic factors, could prevent or halt the progress of neurodegeneration in Parkinson’s disease (PD). In this study, we investigated the therapeutic accomplishment of geraniol (GE 100 mg/kg) on 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced mice model of PD. Current investigation proved that pretreatment with GE ameliorates the MPTP-induced alterations in behavioral, biochemical, immunohistochemical, and immunoblotting manifestations in mice. Systematically, the loss of dopaminergic neurons and reduced NTFs mRNA expressions induced by MPTP was ameliorated to a significant extent by pretreatment with GE. We found that GE confers a potent neuroprotective agent against MPTP-induced dopaminergic denervation and may become a potential therapeutic agent for PD and/or its progression.


Introduction
Parkinson's disease (PD) is one of the most studied neurological diseases (Wu and Frucht 2005) characterized by a progressive degeneration of dopaminergic neurons in substantia nigra (SN) (Collier et al. 2011). The loss of SN neurons leads to dopamine (DA) depletion in the striatum (ST), which results in motor impairments. Nowadays, many animal models of PD are obtainable (Hirsch 2007) by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyrindine (MPTP) and 6-hydroxydopamine (Schober 2004;Schwarting and Huston 1996). The MPTP model constitutes the best characterized toxin paradigm for PD, faithfully replicating most of its clinical and pathological hallmarks (Eberhardt and Schulz 2003). Since it is a lipophilic molecule that crosses the blood-brain barrier and converted to 1-methyl-4-phenylpyridinium ion (MPP + ), which is taken up by dopaminergic terminals and cell bodies by the dopamine transporter (DAT) (Smeyne and Jackson-Lewis 2005). It accumulates in mitochondria resulting in oxidative stress and cell loss (Nicholas 2007;Przedborski et al. 2004;Wu et al. 2002).
Microglia are the major resident immune cells in the brain providing innate immunity; however, astrocytes and oligodendrocytes are also involved in the neuroinflammatory response (Tansey et al. 2007). It maintains the homeostasis of the brain through the production of various NTFs. NTFs, such as brain-derived neurotrophic factor (BDNF), insulinlike growth factor 1, glial cell-derived neurotrophic factor (GDNF), and neurotrophin nerve growth factor that are important for the survival, maintenance, and regeneration of specific neuronal populations in the adult brain (Mogi et al. 1999).
The neuroprotective or neurorescue activities of these NTFs have been demonstrated in a number of in vitro and in vivo experiments (Peterson and Nutt 2008). The depletion of these neurotrophic factors has been linked with disease pathology and symptoms of PD. BDNF accelerate DA turnover in the ST and plays an important role in maintaining the normal function and regeneration of DA neurons in the adult brain (Lindsay et al. 1994). GDNF is a member of the transforming growth factor-β super family of neurotrophic factor (Saarma 2000). It is required for the survival of dopaminergic neurons (Lin et al. 1993) and promotes recovery in rodent and nonhuman primate models of PD (Kordower et al. 2000;Dowd et al. 2005). GDNF levels are decreased in the SN of PD patients (Jenner and Olanow 1998) and in normal aged rodents (Yurek and Fletcher-Turner 2001), suggesting its involvement in motor dysfunction and DA neuron degeneration. It has been hypothesized that neurotrophic factors might have the ability to restore the function of dopaminergic neurons (Hong et al. 2008;Yang et al. 2009). However, these factors cannot reach their target areas in the brain by systematic administration. Therefore, an important new research field which involves the search for small molecules that can enter the brain tissue and then trigger the endogenous neuroprotective mechanisms (Xu et al. 2010) is the potential therapeutics for neurodegenerative diseases.
Geraniol (GE), an acyclic monoterpene alcohol found in lemongrass and aromatic herb oils, proved to have cytoprotective and antioxidant potential in oxidative stress-induced animal models (Tiwari and Kakkar 2009). It also modulates the activity of enzymatic and nonenzymatic antioxidants (Madankumar et al. 2013). The protective effect of this compound against oxidative stress will open new insights for their therapeutic use in inflammatory diseases and other diseases where oxidative stress is a crucial event. Consequently, in the present investigation, we demonstrate the neuroprotective effects of GE on MPTP-induced behavioral deficits, oxidative stress, and alter the expressions of NTFs, tyrosine hydroxylase (TH), DAT, and vesicular monoamine transporter 2 (VMAT2) in a mouse model of PD.

Animals
Ten-week-old male C57BL/6 mice (25-30 g) purchased from the National Institute of Nutrition, Hyderabad were used in the present study. The animals were group-housed two animals per cage in a 12:12 h light/dark cycle with free access to food pellets and water. All experimental procedures were conducted according to the National Guidelines on the Proper Care and Use of Animals in Laboratory Research (Indian National Science Academy, New Delhi, 2000) and were approved by the animal ethics committee of the institute (reg. no. 160/1999/ CPCSEA; approval no: 881/2012) RMMC.

Experimental Design and Dosage Fixation
A pilot study was conducted with four different doses of GE (50, 100, and 200 mg/kg) to determine the dose-dependent effect of GE in MPTP-induced PD mice. It was observed that after the experimental period of 7 days, GE oral pretreatment at the doses of 50, 100, and 200 mg/kg significantly improved the levels of dopamine and its metabolites in MPTPinduced mice. From the results, it was observed that 100 and 200 mg/kg of GE administration showed similar induction in dopamine levels but more significant than 100 mg/kg. As per result, we have selected the optimum dose (100 mg/kg) for our acute study.
The mice were randomized and divided into 4 groups of 18 mice each. Six mice in each group were used for biochemical analysis, another six mice were used for catecholamine, and the remaining mice were used for molecular studies (immunohistochemical studies, mRNA expressions, and western blot analysis). We followed the safety guidelines for handling of MPTP as reported previously (Lau et al. 2005).
Group I mice were treated with saline served as control. Groups II and III mice received intraperitoneal injection of MPTP (30 mg/kg body weight (bw); Lin et al. 2004) for 4 days consecutively starting from 4th to 7th day of the experimental period. In addition, group III mice received GE (100 mg/kg bw in absolute ethanol) orally (1 h prior to each MPTP injection) for 7 days consecutively, starting from 1st to 7th day of the experimental period. Group IV mice received GE (100 mg/kg) dissolved in absolute ethanol and administered for 7 days by oral gavage. At the end of the experiment (8th day), the following behavioral tests were performed.

Rotarod Performance
The rotarod test, in which animals walk on a rotating rod, is widely used to assess motor status in laboratory rodents. Performance is measured by the duration that an animal stays upon the rod as a function of rod speed. Here, we report that the task provides a rich source of information. Mice were allowed to adjust their posture in order to maintain their balance on a rotating rod at speeds of 5, 10, 15, and 20 rpm. The average retention time on the rod was calculated as described previously (Rozas et al. 1998).

Drag Test
This test was performed to measure the ability of the animal to balance its body posture using forelimbs in response to an externally imposed dynamic stimulus (Viaro et al. 2008). Each mouse was gently lifted using the tail (allowing the forepaws on the table) and dragged backwards at a constant speed (about 20 cm/s) for a fixed distance (120 cm). The number of touches made by each forepaw was counted by two independent observers (mean between the two forepaws).

Footprint Analysis
The footprint analysis was performed as described previously (Tillerson et al. 2002) with slight modification. Briefly, the mice were trained to run toward an enclosed square box in an open-top runway. The forepaws and hind paws were dipped with nontoxic paints, and the mice were immediately placed on one end of the sheet of paper opposite to the square box. The footprint patterns were analyzed for stride length by calculating from the mid digit toe of the first step to the heel of the second step.

Biochemical Studies
Lipid Peroxidation Assay: Malonaldehyde Malonaldehyde (MDA), the final products of lipid peroxidation (LPO), was measured by a colorimetric assay using the Bioxytech LPO-586 colorimetric kit assay (OxisResearch, Portland, OR, USA) (Escames et al. 1997). Briefly, ST tissue samples were homogenized in 20 volumes (w/v) of ice cold 20 mM Tris HCl buffer, pH 7.4. The homogenates were centrifuged at 1000×g for 10 min at 4°C. Aliquots of the supernatant was incubated at 45°C for 40 min in the presence of N-methyl-2-phenylindole/methanol and methanesulfonic acid. The reaction was stopped by chilling samples on ice and centrifuged at 12,000×g for 5 min at 4°C. The absorbance in the supernatant was estimated spectrophotometrically at 586 nm, and concentrations were calculated from a standard curve made with known amounts of MDA.
Reduced Glutathione The level of reduced glutathione (GSH) in the brain homogenate was measured by the method described by Jollow et al. (1974). Brain tissue homogenate was centrifuged at 16,000×g for 15 min at 40°C. The supernatant (0.5 ml) was added to 4 ml of ice-cold 0.1 mM solution of 5, 5-dithiobis[2-nitrobenzoic acid] in 1 M phosphate buffer (pH 8). The optical density was read at 412 nm in a spectrophotometer.

Striatal Dopamine and Its Metabolites
The levels of DA, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) were determined by HPLC apparatus with an electrochemical detector (Muralikrishnan and Mohanakumar 1998). Briefly, the striatum was sonicated in ice-cold 0.1 M HClO4 containing 0.01 % EDTA. The supernatant collected after a spin of 10,000×g for 5 min was injected (10 ml) into the HPLC system. Results were expressed as nanogram per milligram wet weight of brain tissue.

Immunohistochemistry
For immunohistochemical analysis, mice were deeply anesthetized with ketamine immediately after the behavioral test and were intracardially perfused with saline, followed by 4 % of the fixative solution (formaldehyde in 0.1 M phosphate buffer, pH 7.4). The brain (SN) was sectioned coronally at 5 μm thickness on a cryostat. Sections were collected in 0.01 M phosphate-buffered saline (PBS) and processed. Sections were collected in 0.01 M PBS and processed free floating (Hartmann et al. 2001). The latter sections were incubated with 0.3 % hydrogen peroxide for 10 min at room temperature to remove the endogenous peroxidase activity and then placed in blocking buffer containing 10 % normal goat serum (NGS) with 0.2 % Triton X-100 in 0.01 M PBS (pH 7.2) for 30 min at 37°C. In each treatment, the slides were washed at least three times with 0.01 M PBS each for 5 min. Sections were incubated for 24 h with primary anti-mouse TH (1:1,000) in 2 % NGS, 0.2 % Triton X-100, and 0.02 % sodium azide in Tris-buffered saline (TBS). After washing with 1 % NGS in TBS, the sections were incubated in anti-mouse IgG-HRP conjugated antibody (1:1,000) in 1.5 % NGS for 1 h. TH immunoreactivity was visualized in SN after incubation in 3,4-diaminobenzidine for 2-5 min. Sections were mounted with DPX mounting medium and cover slip.
The intensity of TH immunoreactivity in the SN was quantified by optical density measurements using the MicroComputer Imaging Device software; data were presented as a percent of the control group values. The number of TH immunoreactive cells on each representative mesencephalic section was counted for the SN region by persons who were blind to the treatment. Cell counts were determined every sixth section (total 8-10 sections) through SN corresponding to the bregma −2.92 to −3.64 mm from each of the animals, and three animals/group were used for cell counts. All raw cell counts were adjusted with a correction formula for cell size and section thickness according to the method of Abercrombie (1946). When counting was complete, the slides were decoded and arranged based on treatment group. The cell counts were then averaged for each animal, and these averages were used to calculate a mean ± SD for each treatment group, and data were presented as a percent of the control group.

Extraction of Total mRNA
After the 24 h last GE administration, the mice were sacrificed, the brains were removed immediately, and the ST was dissected on ice and immediately transferred to dry ice to preserve mRNA integrity. These regions were used to analyze the dopaminergic nigrostriatal pathway affected by MPTPinduced neurodegeneration. Total mRNA was isolated from the ST using mRNA extraction kit (Genei Bangalore, India), following the manufacturer's instructions. The mRNA integrity was determined by agarose gel electrophoresis, and the concentration and purity were measured spectrophotometrically (Kingston et al. 1996).

Real Time-PCR
The specific mRNA expressions were performed by real-time PCR, by subjecting the resulting cDNA to PCR amplification using 96-well optical reaction plates in the Eppendorf (Thermocycler) real-time PCR instrument, software version V1.5.0.39 (Genei Bangalore, India). Twenty-five microliter of reaction mixture contained 0.1 μl of 10 μM forward primer and 0.1 μl of 10 μM reverse primer (40 nM final concentration of each primer), 12.5 μl of Red Eye RT Mastermix, 11.05 μl of nuclease-free water, and 1.25 μl of cDNA sample. The primers used in the current study were chosen from previously published studies and are listed above. Assay controls were incorporated on to the same plate, namely, no template controls to test for the contamination of any assay reagents. After sealing the plate with an optical adhesive cover, the thermocycling conditions were initiated at 95°C for 10 min, followed by 40 PCR cycles of denaturation at 95°C for 15 s, and anneal/ extension at 60°C for 1 min. Melting (dissociation stage) was performed by the end of each cycle to ascertain the specificity of the primers and the purity of the final PCR product.

Western Blotting
Striatal synaptosomes were prepared according to the methods described by Wright et al. (1998). In brief, ST tissue was homogenized in an ice-cold RIPA buffer (1 % Triton, 0.1 % SDS,0.5 % deoxycholate, 1 mmol/L EDTA, 20 mmol/ L Tris (pH 7.4),150 mmol/L NaCl, 10 mmol/L NaF, and 0.1 mmol/L phenyl-methyl sulfonyl fluoride). The homogenate was centrifuged at 12, 000 rpm/min for 15 min at 4°C to remove debris. Protein concentration was measured by the method of Lowry et al. (1951). Samples containing 40 μg of total cellular proteins were loaded and separated on 10 % SDS polyacrylamide gel electrophoresis. The gel was then transferred on to a PVDF membrane (Millipore). The membranes were incubated with the blocking buffer containing 5 % nonfat dry milk powder or bovine serum albumin for 2 h to reduce nonspecific binding sites and then incubated in mouse anti-GDNF, BDNF (1:200, Santa Cruz Biotechnology, USA), TH (1:1,500, Santa Cruz Biotechnology, USA), DAT(1:500), and VMAT2 (1:1,000) with gentle shaking overnight at 4°C. After this, membranes were incubated with their corresponding secondary antibodies (anti-rabbit or anti-mouse IgG conjugated to horseradish peroxidase) for 2 h at room temperature. The membrane was washed thrice with Tris-buffered saline and 0.05 % Tween-20 for 30 min. Protein bands were visualized by an enhanced chemiluminescence method using ECL kit (GenScript ECL kit, USA). Densitometric analysis was performed with a computer using a gel image analysis program.

Statistical Analysis
All the data were expressed as mean ± SD of number of experiments (n =6). Statistical significance was evaluated by one-way analysis of variance using SPSS version 15.0 software, and individual comparisons were obtained using Duncan's multiple range test. Values were considered statistically significant if P <0.05.

Effect of GE on Behavioral Recovery
The results of spontaneous motor activity performance by behavioral tests were shown in Figs. 1, 2, and 3, respectively. Compared with control mice, the MPTP-treated mice displayed a significant decrease in spontaneous motor activity by latency to fall of the rotarod test, reduce forepaw stride distance, and decreased the number of steps in footprint test (P <0.05). However, GE pretreatment significantly ameliorated these behavioral deficits induced by MPTP toxicity (P <0.05)

Effects of GE on the Levels of MDA and GSH in the ST
To understand the mechanisms of the protective effects of GE, we measured the activities of GSH and the MDA contents in the ST of every group of mice. The results of these biochem-ical parameters were shown in Table 1. The contents of MDA in the ST of MPTP-treated mice were significantly increased compared with those in the control group (P <0.05). However, the increase in MDA level was significantly ameliorated when the mice received GE pretreatment (P <0.05). In addition, the GSH activity in MPTP-treated mice was significantly decreased compared with control group (P <0.05), and GE pretreatment largely attenuated the decrease in GSH (P <0.05).

Effects of GE on the Levels of Dopamine and Its Metabolites in the Striatum
The results of catecholamine measurement were shown in Table 2. The present study confirmed that catecholamine levels Fig. 1 Rotarod performance after the acute regimen of GE and MPTP: pretreatment with GE improved MPTP-induced motor deficits. Mice were tested for motor function using the rotarod (motor function) test in different rpm (5, 10, 15, and 20 rpm). Values are given as mean ± SD for six mice in each group. a P <0.05 compared to the control, b P <0.05 compared to the MPTP control Fig. 2 Drag test performance after the acute regimen of GE and MPTP: Pretreated with GE to MPTP group a profound improvement in sensory motor performance. Values are given as mean ± SD for six mice in each group. a P <0.05 compared to the control, b P <0.05 compared to the MPTP control were significantly decreased in MPTP-treated mice as compared to control mice. Pretreatment with GE following MPTP exposure significantly attenuated the decrease in the levels of DA, DOPAC, and HVA (P <0.05). However, treatment with GE alone in MPTP-treated mice did not change the levels of DA and its metabolites as compared to control mice.

Effect of GE on MPTP-Induced Reduction of TH Immunoreactivity in the SN
Representative microphotographs of TH immunostaining in the SN were shown in Fig. 4a, b. Animals that received the treatment of MPTP injection showed a marked loss of THimmunopositive neurons in the SN compared with the control group. In contrast, GE-treated mice showed significantly reduced nigrostriatal dopaminergic neuron loss following MPTP injection at the dose of 30 mg/kg as compared to MPTP alone treated group (P <0.05).

The Expression of BDNF and GDNF in the MPTP Lessoned ST
The mRNA and proteins expressions of BDNF and GDNF revealed that the MPTP treatment significantly decreased mRNA (Fig. 5a, b) and protein expression level (Fig. 6a, b) of BDNF and GDNF as compared to control group, but GE pretreatment retained both expression and production of BDNF and GDNF activity as compared to the MPTP-treated group of mice (P <0.05). No significant changes were observed between control and GE alone treated mice.
The Effect of GE on MPTP-Induced TH, DAT, and VMAT2 Level in ST As shown in Fig. 7a, b, MPTP treatment significantly decreased the protein expression of TH, DAT, and VMAT2 in ST as compared to control group (P <0.05). GE pretreatment restored TH, DAT, and VMAT2 protein generation as compared to the MPTP-treated group of animal (P <0.05). No significant changes were observed between control and GE alone treated mice.

Discussion
In this study, we demonstrated that GE, which is capable of traversing the cellular membrane and can suppress MPP + (toxic metabolite from MPTP)-induced lipid peroxidation and mRNA expressions and protect dopaminergic neurons in the ST of the brain from MPTP insult through upregulating NTFs and dopaminergic transporters in mice. As a novel astrocyte modulating agent, GE has a potent neuroprotective affect in mice model PD providing evidence that astrocytes can be a new target of neuroprotection. Intraperitoneal administration of neurotoxin MPTP induces more obvious loss of dopaminergic neurons in the ST and SN in C57BL/6 mice (Filipov et al. 2009;Yasuda et al. 2008). Mice were sacrificed after the last behavioral assessment, and the striatum was dissected for biochemical evaluation. All values are expressed as mean ± SD a P <0.05 compared with control group b P <0.05 compared with MPTP treatment group Mice were sacrificed after the last behavioral assessment, and the striatum was dissected for dopamine and its metabolites (DOPAC and HVA) evaluation. All values are expressed as mean ± SD a P <0.05 compared with control group b P <0.05 compared with MPTP treatment group Fig. 3 Comparison of behavioral output between control and MPTPtreated mice. The footprint patterns were calculated from the stride length, mid digit toe of the first step, to the heel of the second step. The GE pretreatment improves the stride length after MPTP treatment. Values are given as mean ± SD for six mice in each group. a P <0.05 compared to the control, b P <0.05 compared to the MPTP control Our experiments on the behavioral study suggesting that MPTP-induced DA depletion relate specifically to the diminished muscular coordination and balance (Moon et al. 2009) rather than an influence on the motor capacity of mice.
The behavioral effects are intertwined with the degree of dopaminergic neuronal dysfunction (Schwarting et al. 1991); its assessment is a more powerful endpoint in evaluating neuroprotection against degeneration. Therefore, testing the Fig. 4 a Effect of GE on TH expression in SN. Representative microphotographs of the expression of TH was almost negligible in MPTP group as compared to control group, while the MPTP group treated with GE has shown a moderate staining of TH. However, the GE control group has shown no discernible change in TH staining as compared to control group. Original magnifications at ×4 and ×40. b Quantification of TH-IR was performed by counting the number of TH-IR neurons in SN. The mean value for TH-IR was determined for each group and was expressed as a percentage of that matched control mice. Values are expressed as mean±SD of three mice per group. a P <0.05, compared with the control. b P <0.05, compared with the MPTP control group behavioral function in the current study provides a sensitive evaluation of new therapeutic agent without any undesirable effects. Our study results suggest that acute MPTP injections caused severe motor deficits as assessed by rotarod, foot print analysis, and drag test in mice after the last MPTP administration. Pretreatment with GE significantly alleviate these behavior deficits by improved motor coordination. Moreover, it was revealed that this effect of GE was closely associated with the protection of nigrostriatal dopaminergic neurons against MPTP-induced neurotoxicity in the brain. Pretreatment with GE was found to improve motor deficits by increase of striatal catecholamine levels after MPTP injection. These behavioral alterations were consistent with a previous report (Sundstrom et al. 1990).
While this study provides evidence against MPTP-induced LPO by evaluating MDA, it is a well-known mechanism of cellular injury initiated by ROS (Sayre et al. 2001). The cytotoxic aldehydes produced in the process of LPO and production could reflect the oxidative damage to lipids and proteins that leads to the pathological process of PD (Esterbauer et al. 1991;Alam et al. 1997). We revealed that pretreatment with GE evidently decreases the formation of MDA of MPTP-treated mice ST tissue. GSH is the most important thiolcontaining antioxidant in the brain (Meister and Anderson 1983), and it plays a pivotal role in preventing oxidative damage. It also has been used as a biomarker of oxidative stress in biological systems (Reed and Savage 1995). Depletion of GSH has been observed in the ST of PD patients and in the MPTP model (Ferraro et al. 1986). These results demonstrated that GE restore the MPTP-induced oxidative insults in striatal systems, and it confers potent protection of GE for the DA neurons by acting in both directions to restore the balance of oxidant accumulation and clearance.
Additionally, present findings are in agreement with the earlier reports that DA level and motor deficits in Parkinsonian mice have been attenuated by antioxidant supplementation (Chung et al. 2011;Khan et al. 2010;Moon et al. 2009). The degrees of DA depletion are established by the evaluation of TH levels, the rate limiting enzyme of DA biosynthesis, as an indirect indicator of DA, which could be markedly decreased by MPTP (Stephenson et al. 2007;Chen et al. 2009). The observed result showed that the administration of MPTP in ST could reach a modest but statistical significance to reduce the level of TH in ST. GE effectively reversed the reduced expression of TH and Fig. 6 a Western blot analysis of BDNF and GDNF expression levels in ST. Western blot was performed to study the protein expression levels of BDNF and GDNF in the striatal tissue after GE treated and GE non-treated mice. b Bar graph shows the comparison among the group, and density was quantified by scanning densitometry. Values are expressed as mean±SD of three mice per group. a P <0.05, compared with the control. b P <0.05, compared with the MPTP control group prevented gradual loss of neurons in SN and ST. DAT and VMAT2, two different transporters for DA and the dopaminergic toxins, had been regarded as injury index of dopaminergic neurons, which is helpful for early detection and intervention of PD. DAT is a critical regulator of DA distribution within the brain and is also a crucial determiner of the neurotoxicity of MPP + . DAT downregulation was probably a long-term compensatory mechanism directed at maintaining DA input in the ST (Afonso-Oramas et al. 2010) or preventing toxin to enter into neurons (Bezard et al. 2003). The study demonstrated that one component of the neuroprotection conferred by GE is to regulate the expression of DAT and VMAT2 in the MPTP-induced mice, which may influence the amount of neurotoxin uptake and the degree of lesion. Otherwise, the reduction of DAT may be correlated which influenced the transport of monoamine transmitter. The VMAT2 may serve as a neuroprotective factor by sequestering monoamines into vesicles and preventing the interaction with their catabolic enzymes (Caudle et al. 2008). In the present study, a decreased expression of DAT and VMAT2 were observed in the ST of MPTP-treated mice, which corresponds to the results of previous reports (Erickson et al. 1996;Weihe and Eiden 2000;Kurosaki et al. 2003).
The neuronal survival requires NTFs support. A limited availability of NTFs has been suggested to contribute to the mechanism of DA neurodegeneration in PD (Mogi et al. 1999), while they promote dopamine neurorescue in the mice and nonhuman MPTP models (Date et al. 1998;Lapchak et al. 1998;Wang et al. 2002). BDNF and GDNF are the most promising trophic factors in neurorescue or restorative treatment of neurodegenerative diseases, particularly in PD (Anand 2004;Bespalov and Saarma 2007). The development of therapeutic drug that modulate the functions of these factors, study from our laboratory established that a partial loss of GDNF and BDNF mice decline in motor function and the DA system The band density was quantified by scanning densitometry. Bar graph shows the comparison among groups. Values are expressed as mean ± SD of three mice per group. a P <0.05, compared with the control. b P <0.05, compared with the MPTP control group (Boger et al. 2006(Boger et al. , 2007. Furthermore, we found that pre intake of GE markedly counteracted neurotoxic effects of MPTP and restored the expression, activity, and generation of BDNF and GDNF, which benefited dopamine synthesis in brain. These findings implied that GE could prevent PD progression through regulating neurotrophic factors, stabilizing TH, and protecting dopaminergic neurons, which consequently favored dopamine formation (Tsai et al. 2011).

Conclusion
In conclusion, the present study corroborated that the neuroprotective efficacy of GE in MPTP induced neurotoxicity. The protective effects were affirmed by improved motor coordination, expressions of NTFs, and inhibiting oxidative stress, increasing the counts of dopaminergic immunoreactive neurons. Based on the current result of our investigation, we speculate that GE might be a promising contender for the prevention or treatment of oxidative stress-related neurodegenerative disorders such as Parkinson's disease, but further studies are warranted to determine the possible role of GE in neurodegenerative process.

Conflict of Interest No conflict of interest
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