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Treadmill Exercise Decreases Inflammation Via Modulating IL-6 Expression in the Rat Model of Middle Cerebral Artery Occlusion

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Abstract

Background

Long-term bed rest in neurointensive care (NIC) patients leads to skeletal muscle atrophy and cognitive dysfunction, which seriously affects the physical fitness and final prognosis of critically ill patients. Exercise therapy plays an increasingly important role in the treatment and rehabilitation of patients with sarcopenia. However, the therapeutic effect and mechanism of exercise therapy for patients with neurological impairment remain unclear.

Methods

Serum samples of NIC patients before and after exercise therapy and normal people were collected to detect interleukin-6 (IL-6) and interleukin-1β levels by enzyme-linked immunosorbent assay (ELISA). Middle cerebral artery occlusion (MCAO) was used for the construction of a rat model. The Morris water maze test, exploration test, and open-field test were used to assess neurological function in rats. Western blot and quantitative real-time polymerase chain reaction were performed to evaluate the activation of IL-6/adenosine-monophosphate-activated protein kinase (AMPK) signaling.

Results

Exercise therapy attenuated IL-6 expression in NIC patients. Exercise therapy alleviated cognitive dysfunctions and decreased IL-6 expression in MCAO rats. Exercise therapy alleviated gastrocnemius muscle injury in rats after MCAO by modulating IL-6/AMPK signaling.

Conclusions

Treadmill exercise decreases inflammation in MCAO rats via modulating IL-6/AMPK signaling.

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Authors and Affiliations

  1. Department of Rehabilitation, The Affiliated Jiangning Hospital of Nanjing Medical University, No. 169 Hushan Road, Dongshan Street, Jiangning District, Nanjing, 210000, Jiangsu, China

    Hui Feng, Xinlong Wang & Fangyu Chen

  2. Department of Hyperbaric Oxygen, The Second People’s Hospital of Hefei, Hefei Hospital Affiliated to Anhui Medical University, Hefei, 230011, Anhui, China

    Yinliang Qi

  3. Department of Rehabilitation, Nanjing First Hospital, Nanjing Medical University, No. 68 Changle Road, Qinhuai District, Nanjing, 210000, Jiangsu, China

    Xueping Li

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  1. Hui Feng
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  3. Xinlong Wang
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  4. Fangyu Chen
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Contributions

HF, YQ, XW, FC, and XL did the experiments, collected and analyzed the data, and wrote the manuscript. HF and XL conceived and coordinated the study.

Corresponding author

Correspondence to Hui Feng.

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Conflict of interest

Hui Feng, Yinliang Qi, Xinlong Wang, Fangyu Chen, and Xueping Li declare that they have no competing interest.

Ethical Approval

The study was approved by the Ethics Committee of The Affiliated Jiangning Hospital of Nanjing Medical University.

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Feng, H., Qi, Y., Wang, X. et al. Treadmill Exercise Decreases Inflammation Via Modulating IL-6 Expression in the Rat Model of Middle Cerebral Artery Occlusion. Neurocrit Care 38, 279–287 (2023). https://doi.org/10.1007/s12028-022-01575-3

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  • DOI: https://doi.org/10.1007/s12028-022-01575-3

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