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Can signal peptide-CUB-EGF domain-containing protein 1 (SCUBE-1) be used as an indicator of endothelial dysfunction in acromegaly patients?

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Abstract

Purpose

Acromegaly is closely related to increased oxidative stress and endothelial dysfunction (ED). This study aimed to evaluate, for the first time in the literature, signal peptide-CUB-EGF domain-containing protein 1 (SCUBE-1) and endothelial nitric oxide synthase e(NOS) levels in the setting of acromegaly.

Method

A total of 56 acromegaly patients and a control group composed of 30 healthy volunteers were included in this study. In the postoperative follow-up, patients were grouped as active or in-remission according to their GH and IGF-1 levels in oral glucose stimulation test (OGST). After detailed physical examination of acromegaly patients and the control subjects, 8-hour fasting blood samples were collected to evaluate biochemical parameters including lipid profile, anterior pituitary hormones, and SCUBE-1 and e(NOS) levels.

Results

Inactive and active acromegaly was noted in 78.6% and 21.4% of patients, respectively. The median (min-max) SCUBE-1 levels were significantly higher in the inactive acromegaly and active acromegaly groups than in the control group (1.6(0.4–2.4) and 1.8(1.1–2.5) vs. 0.4(0.2–1.0) ng/mL, respectively, p < 0.001 for each). The median (min-max) e(NOS) levels were significantly higher in the inactive acromegaly and active acromegaly groups than in the control group (132.7 (26.8–602.9) and 137.3 (69.7–488.7) vs. 83.9 (16.4–218.7) pg/mL, p = 0.018 and p = 0.048, respectively). We have also detected positive correlations of e(NOS) with leukocyte (r = 0.307, p = 0.021) and neutrophil counts (r = 0.309, p = 0.021).

Conclusion

Our study revealed for the first time in literature that SCUBE-1 levels, being a novel marker for ED, were significantly higher in acromegaly patients than in control subjects. When supported with clinical studies, SCUBE-1can be used as an early indicator of endothelial damage in acromegaly patients.

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism, Recep Tayyip Erdogan University Faculty of Medicine, Rize, Turkey

    Damla Tufekci

  2. Department of Endocrinology and Metabolism, Karadeniz Technical University Faculty of Medicine, Trabzon, Turkey

    Irfan Nuhoglu, Hulya Coskun, Muhammet Cuneyt Bilginer, Ozge Ucuncu & Mustafa Kocak

  3. Department of Biochemistry, Karadeniz Technical University Faculty of Medicine, Trabzon, Turkey

    Sumeyye Sura Ayan, Serap Ozer Yaman & Suleyman Caner Karahan

  4. Clinics of Endocrinology and Metabolism, Giresun Training and Research Hospital, Giresun, Turkey

    Yasemin Emur Gunay

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Contributions

CRediT authorship contribution statement. Conceptualization: D.T., I.N., S.C.K. Methodology: D.T., I.N., H.C. Data curation: D.T., I.N., H.C., M.K., O.U., C.B., Y.E.G. Formal analysis: S.S.A., S.C.K., S.O.Y., M.C.B. Investigation: S.C.K., M.K., O.U. Project administration: S.S.A. Writing-original draft preparation: D.T., S.O.Y., M.C.B. Writing- review & editing: D.T., M.K., H.C., M.C.B. Supervision: S.S.A., O.U., M.C.B.

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Correspondence to Damla Tufekci.

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Written informed consent was obtained from each subject following a detailed explanation of the objectives and protocol of the study.

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The study protocol was approved by the Karadeniz Teknik University Ethics Committee (Date of Approval : 30/06/2021, Protocol no:2021/595-24237859).

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Tufekci, D., Nuhoglu, I., Ayan, S.S. et al. Can signal peptide-CUB-EGF domain-containing protein 1 (SCUBE-1) be used as an indicator of endothelial dysfunction in acromegaly patients?. Endocrine 82, 152–160 (2023). https://doi.org/10.1007/s12020-023-03444-1

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