Abstract
We measured the intracellular zinc ion concentration of murine fetal neural stem/progenitor cells (NSPCs) and that in the differentiated cells. The NSPCs cultured with 1.5 μM Zn2+ proliferated slightly faster than that in the zinc-deficient medium and the intracellular zinc concentration of the NSPCs and that of their differentiated cells (DCs) cultured with 1.5 μM Zn2+ was 1.34-fold and 2.00-fold higher than those in the zinc-deficient medium, respectively. The zinc transporter genes upregulated over the 3.5-fold change were Zip1, Zip4, Zip12, Zip13, ZnT1, ZnT8, and ZnT10 whereas the only downregulated one was Zip8 during the differentiation of NSPCs to DCs. The cell morphologies of both NSPCs and DCs in the low oxygen culture condition consisting of 2%O2 and 5%CO2, the high carbon dioxide condition consisting of 21%O2 and 10%CO2, and the normal condition consisting of 21%O2 and 5%CO2 were essentially the same each other. The expression of Zip4, Zip8, Zip12, and Zip14 was not drastically changed depending on the O2 and CO2 concentrations.
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20 January 2024
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Acknowledgements
We express our gratitude to Youtaro Ono for his contribution to the experiment on the low O2 culture and high CO2 culture, and also to Mayu Nishikawa for her contribution to the discussion about ZIPs and ZnTs expression. This work was partially supported by JSPS KAKENHI Grant Numbers JP26420799, and JP20K05236.
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The author’s contribution to this manuscript was described as follows. Dr. H. Mori planned the experiment. Ms. A. Goji took the experimental data. Dr. M. Hara wrote the manuscript.
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Mori, H., Goji, A. & Hara, M. Upregulation of Intracellular Zinc Ion Level after Differentiation of the Neural Stem/Progenitor Cells In Vitro with the Changes in Gene Expression of Zinc Transporters. Biol Trace Elem Res (2024). https://doi.org/10.1007/s12011-023-04033-z
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DOI: https://doi.org/10.1007/s12011-023-04033-z