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Effects of Zinc Adaptation on Histological Morphology, Antioxidant Responses, and Expression of Immune-Related Genes of Grass Carp (Ctenopharyngodon idella)

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Abstract

The study was conducted to evaluate the effect of zinc adaptation on histological morphology and antioxidant and immune responses of grass carp(Ctenopharyngodon idella). A total of 180 young grass carp (20.0 ± 2.0 g) was equally distributed into 9 groups, and triplicate groups were subjected to 0 μg/L Zn2+ (control group), 200 μg/L Zn2+, and 300 μg/L Zn2+ solution for 42 days, respectively. The results indicated that the liver and gill have obvious pathological changes after long-term adaptation to zinc except the intestine; the zinc adaptation can positively influence intestinal morphology. The activities of GPX (glutathione peroxidase activity), SOD (superoxide dismutase), and CAT (Catalase) were significantly increased in zinc treatment groups (P < 0.05). The genes expression levels of CuZnSOD (copper zinc superoxide dismutase), CAT, Hsp70 (heat shock protein-70), IL-1b (interleukin-1-b), and TGF-β1 (transforming growth factor-β1) were upregulated in the gill and intestine of grass carp following waterborne adaptation to zinc solution for 42 days (P < 0.05). In conclusion, zinc adaptation has different effects on organs of grass carp and may reduce the inflammatory response of the body’s gills and intestines by improving the body’s antioxidant and anti-stress defense capabilities.

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Data availability

The data that support the findings of this study are available from the corresponding author Hongtao Ren upon reasonable request at Animal Science and Technology College, Henan University of Science and Technology, People’s Republic of China.

Code availability

Not applicable.

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Acknowledgements

We would like to thank the postgraduate students of Animal Science and Technology College, Henan University of Science and Technology, People’s Republic of China, for their help throughout the research period.

Funding

This work was supported by the Henan Natural Science Foundation (182300410032).

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Authors and Affiliations

  1. Animal Science and Technology College, Henan University of Science and Technology, Luoyang, 471003, China

    Hong tao Ren, Hui ying An, Ming xing Du & Jian Zhou

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  1. Hong tao Ren
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  2. Hui ying An
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  4. Jian Zhou
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Contributions

Hongtao Ren designed the project, analyzed the data, and wrote the manuscript. Hui ying An, Ming xing Du, and Jian Zhou performed the experiments and collected the data.

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Correspondence to Hong tao Ren.

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All experimental protocols were approved by the Bioethical Committee of Henan University of Science and Technology.

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Ren, H.t., An, H.y., Du, M.x. et al. Effects of Zinc Adaptation on Histological Morphology, Antioxidant Responses, and Expression of Immune-Related Genes of Grass Carp (Ctenopharyngodon idella). Biol Trace Elem Res 200, 5251–5259 (2022). https://doi.org/10.1007/s12011-022-03112-x

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