Abstract
The experiment was conducted to investigate the effect of supplementary zinc-threonine (Zn-Thr), zinc-methionine (Zn-Met), and zinc oxide (ZnO) on the laying performance, egg quality, Zn content in egg and excreta. One hundred and sixty hens (Hy-Line W36) were randomly divided into 10 treatments with 4 replications of 4 hens each. During the first 4 weeks, groups were fed a corn-soy basal diet without extra zinc (30.3 mg Zn/kg diet) to depletion storage zinc from the body and it was used as the control diet. During the ensuing 10 weeks from 36 to 45 weeks of age, 3 levels of 30, 60, and 90 mg Zn/kg were added to the diet by ZnO, Zn-Met, and Zn-Thr sources. Results showed that there were no significant differences between the experimental treatments in terms of egg weight, feed intake, eggshell weight, eggshell percentage, and albumen weight. In total experimental period, the supplementation of Zn-Met and Zn-Thr decreased feed conversion ratio and increased egg mass and egg production compared with ZnO 30 and 60 mg Zn/kg and control treatments (P < 0.05). The results showed that treatment effect on Zn content in egg and excreta was significant (P < 0.05). It is concluded that diets supplied with lower levels of organic Zn can maintain egg production performance, improve egg quality, enrich eggs, increase bioavailability, and reduce the negative effects of age on egg quality characteristics in laying hens compared with their oxide form.
Similar content being viewed by others
References
Olgun O, Yildiz AÖ (2017) Effects of dietary supplementation of inorganic, organic or nano zinc forms on performance, eggshell quality, and bone characteristics in laying hens. Ann Anim Sci 17(2):463–476
Hotz C, Brown KH (2004) Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr Bull 25:99–129
Virden WS, Yeatman JB, Barber SJ, Willeford KO, Ward TL, Fakler TM, Kidd MT (2002) Responses of chicks from broiler breeders fed supplemental zinc and manganese: cellular immunity. Poult Sci 81(1):119
Virden WS, Yeatman JB, Barber SJ, Willeford KO, Ward TL, Fakler TM, Wideman RF Jr, Kidd MT (2004) Immune system and cardiac functions of progeny chicks from dams fed diets differing in zinc and manganese level and source. Poult Sci 83(3):344–351
Mabe I, Rapp C, Bain MM, Nys Y (2003) Supplementation of a corn-soybean meal diet with manganese, copper, and zinc from organic or inorganic sources improves eggshell quality in aged laying hens. Poult Sci 82(12):1903–1913
Park SY, Birkhold SG, Kubena LF, Nisbet DJ, Ricke SC (2004) Effects of high zinc diets using zinc propionate on molt induction, organs, and postmolt egg production and quality in laying hens. Poult Sci 83(1):24–33
Brown KH, Wuehler SE (2000) Zinc and human health results of recent trials and implications for program interventions and research. Micronutrient Initiative, Canada. pp68
Wedekind KJ, Hortin AE, Baker DH (1992) Methodology for assessing zinc bioavailability: efficacy estimates for zinc-methionine, zinc sulfate, and zinc oxide. J Anim Sci 70(1):178–187
Lim HS, Paik IK (2003) Effect of supplementary mineral methionine chelates (Zn, Ca and Mn) of performance and eggshell quality of laying hens. Asian-Australas J Anim Sci 16(12):1804–1808
Ao T, Pierce JL, Power R, Dawson KA, Pescatore AJ, Cantor AH, Ford MJ (2006) Evaluation of Bioplex Zn as an organic zinc source for chicks. Int J Poult Sci 5(9):808–811
Yan F, Waldroup PW (2006) Evaluation of Mintrex® manganese as a source of manganese for young broilers. Int J Poult Sci 5(8):708–713
Tronina W, Kinal S, Lubojemska B (2007) Effect of various forms of zinc applied in concentrate mixtures for broiler chickens on its bioavailability as well as meat composition and quality. Pol J Food Nutr Sci 57(4):577–581
Tronina W, Kinal S, Lubojemska B (2007) Effect of various forms of zinc applied in concentrate mixtures for broiler chickens on its bioavailability as well as meat composition and quality. Pol J Food Nutr Sci 57(4):577–581
Swiatkiewicz S, Koreleski J (2008) The effect of zinc and manganese source in the diet for laying hens on eggshell and bones quality. Vet Med – Czech 53(10):555–563
Gheisari AA, Rahimi-Fathkoohi A, Toghyani M, Gheisari MM (2010) Effects of organic chelates of zinc, manganese and copper in comparison to their inorganic sources on performance of broiler chickens. J Anim Plant Sci 6(2):630–636
Britanico EB, Merca FE, Angeles AA, Acda SP, Luis ES (2014) Effects of supplementing diets with amino acid chelates of copper, zinc, manganese and iron on the performance of broilers. Philip J Vet Med 38(1)
Gill C (1997) Organic trace metal assays. Feed international 18(10):18–22
Bahakaim ASA, Magied HAA, Osman SMH, Omar AS, Malak NYA, Ramadan NA (2014) Effect of using different levels and sources of zinc in layer’s diets on egg zinc enrichment. Egypt Poult Sci 34(1):39–56
Yenice E, Mizrak C, Gultekin M, Atik Z, Tunca M (2015) Effects of dietary organic or inorganic manganese, zinc, copper and chrome supplementation on the performance, egg quality and hatching characteristics of laying breeder hens. Vet Fak 62:63–68
Cheng J, Kornegay ET, Schell T (1998) Influence of dietary lysine on the utilization of zinc from zinc sulfate and a zinc-lysine complex by young pigs. J Anim Sci 76(4):1064–1074
Haugh RR (1937) The Haugh unit for measuring egg quality. The U.S Egg & Poult Mag 43:522–555
SAS Institute (2003) SAS/STAT® User’s Guide: Statistics Version 6.12. SAS Institute Inc., Cary, NC
Stefanello C, Santos TC, Murakami AE, Martins EN, Carneiro TC (2013) Productive performance, eggshell quality, and eggshell ultrastructure of laying hens fed diets supplemented with organic trace minerals. Poult Sci 93(1):104–113
Manangi MK, Vazques-Anon M, Richards JD, Carter S, Knight CD (2015) The impact of feeding supplemental chelated trace minerals on shell quality, tibia breaking strength, and immune response in laying hens. J Appl Poult Res 24(3):316–326
Martin KM (2016) The effects of zinc supplementation from two sources on egg quality and bone health in laying hens
Bao YM, Choct M, Iji PA, Bruerton K (2007) Effect of organically complexed copper, iron, manganese, and zinc on broiler performance, mineral excretion, and accumulation in tissues. J App Poult Res 16(3):448–455
Tůmová E, Gous RM (2012) Interaction of hen production type, age, and temperature on laying pattern and egg quality. Poult Sci 91(5):1269–1275
Gheisari AA, Sanei A, Samie A, Gheisari MM, Toghyani M (2011) Effect of diets supplemented with different levels of manganese, zinc, and copper from their organic or inorganic sources on egg production and quality characteristics in laying hens. Biol Trace Elem Res 142(3):557–571
Kidd MT, Anthony NB, Lee SR (1992) Progeny performance when dams and chicks are fed supplemental zinc. Poult Sci 71(7):1201–1206
Tabatabaie MM, Aliarabi H, Saki AA, Ahmadi A, Siyar SA (2007) Effect of different sources and levels of zinc on egg quality and laying hen performance. Pak J Biol Sci 10(19):3476–3478
Kaya Ş, Umucalilar HD, Haliloğlu S, İpek H (2001) Effect of dietary vitamin A and zinc on egg yield and some blood parameters of laying hens. Turk J Vet Anim Sci 25(5):763–769
Durmuş IS, Ataşoğlu C, Mizrak CE, Ertaş S, Kaya MU (2004) Effect of increasing zinc concentration in the diets of Brown parent stock layers on various production and hatchability traits. Arch Anim Breed 47(5):483–489
Amem MH, Al-Daraji HJ (2011) Zinc improves egg quality in Cobb500 broiler breeder females. Int J Poult Sci 10(6):471–476
Silversides FG, Scott AT (2001) Effect of storage and layer age on quality of eggs from two lines of hens. Poult Sci 80(8):1240–1245
Van Den Brand H, Parmentier HK, Kemp AB (2004) Effects of housing system (outdoor vs cages) and age of laying hens on egg characteristics. Br Poult Sci 45(6):745–752
Al-Daraji HJ, Amen MH (2011) Effect of dietary zinc on certain blood traits of broiler breeder chickens. Int J Poult Sci 10(10):807–813
Nakada T, Koja Z, Tanaka K (1994) Effect of progesterone on ovulation in the hypophysectomised hen. Br Poult Sci 35(1):153–156
Skřivan M, Marounek M, Bubancova I, Podsedníček M (2010) Influence of limestone particle size on performance and egg quality in laying hens aged 24–36 weeks and 56–68 weeks. Anim Feed Sci Technol 158(1–2):110–114
Ashmead D (1974) The need for chelated trace minerals. Vet Med Small Anim Clin 69(4):467–468
Nitrayova S, Windisch W, Von Heimendahl E, Müller A, Bartelt J (2012) Bioavailability of zinc from different sources in pigs. J Anim Sci 90(4):185–187
Chan S, Gerson B, Subramaniam S (1998) The role of copper, molybdenum, selenium, and zinc in nutrition and health. Clin Lab Med 18(4):673–685
Ibs KH, Rink L (2003) Zinc-altered immune function. J Nutr 133(5):1452S–1456S
Lima FR, Mabe I, Albuquerque R (2000) Zinc and manganese additional supplementations may benefit egg production and quality. Poult Sci 79(1):46
Abd El-Hack ME, Alagawany M, Amer SA, Arif M, Wahdan KM, El-Kholy MS (2018) Effect of dietary supplementation of organic zinc on laying performance, egg quality and some biochemical parameters of laying hens. J Anim Physiol An N 102(2):e542–e549
Dikmen BY, Sözcü A, İpek A, Şahan Ü (2015) Effects of supplementary mineral amino acid chelate (ZnAA-MnAA) on the laying performance, egg quality and some blood parameters of late laying period layer hens. Vet Fak Derg 21:155–162
Salim HM, Lee HR, Jo C, Lee SK, Lee BD )2011) Supplementation of graded levels of organic zinc in the diets of female broilers: effects on performance and carcass quality. Br Poult Sci 52(5):606–612
Burrell AL, Dozier WA, Davis AJ, Compton MM, Freeman ME, Vendrell PF, Ward TL (2004) Responses of broilers to dietary zinc concentrations and sources in relation to environmental implications. Br Poult Sci 45(2):255–263
Saleh AA, Ragab MM, Ahmed EA, Abudabos AM, Ebeid TA (2018) Effect of dietary zinc-methionine supplementation on growth performance, nutrient utilization, antioxidative properties and immune response in broiler chickens under high ambient temperature. J Appl Anim Res 46(1):820–827
Paik I (2001) Application of chelated minerals in animal production. Asian-Australas J Anim Sci 14(SPI):191–198
Nys Y, Gautron J, McKee MD, Garcia-Ruiz JM, Hincke MT (2001) Biochemical and functional characterisation of eggshell matrix proteins in hens. Worlds Poult Sci J 57(4):401–413
Bai S, Jin G, Li D, Ding X, Wang J, Zhang K, Zeng Q, Ji F, Zhao J (2017) Dietary organic trace minerals level influences eggshell quality and minerals retention in hens. Ann Anim Sci 17(2):503–515
Klecker D, Zeman L, Jelinek P, Bunesova A (2002) Effect of manganese and zinc chelates on the quality of eggs. Acta Univ Agric Silvic Mendel Brun 50:59–68
Stahl JL, Cook ME, Sunde ML (1986) Zinc supplementation: its effect on egg production, feed conversion, fertility, and hatchability. Poult Sci 65(11):2104–2109
Zamani A, Rahmani HR, Pourreza J (2005) Effect of different levels of manganese and zinc on performance traits and breaking eggs in laying hens. Pak J Biol Sci 8(7):1035–1040
Cusack M, Fraser AC, Stachel T (2003) Magnesium and phosphorus distribution in the avian eggshell. Comp Biochem Physiol B Biochem Mol Biol 134(1):63–69
Fernandes JIM, Murakami AE, Sakamoto MI, Souza LMG, Malaguido A, En M (2008) Effects of organic mineral dietary supplementation on production performance and egg quality of white layers. Br Poult Sci 10:59–65
Dunn IC, Joseph NT, Bain M, Edmond A, Wilson PW, Milona P, Nys Y, Gautron J, Schmutz M, Preisinger R, Waddington D (2009) Polymorphisms in eggshell organic matrix genes are associated with eggshell quality measurements in pedigree Rhode Island red hens. Anim Genet 40(1):110–114
Park JA, Sohn SH (2018) The influence of hen aging on eggshell ultrastructure and Shell mineral components. Korean J Food Sci An 38(5):1080
Lee MH, Cho EJ, Choi ES, Sohn SH (2016a) The effect of storage period and temperature on egg quality in commercial eggs. Korean J Poult Sci 43(1):31–38
Wilson PB (2017) Recent advances in avian egg science: a review. Poult Sci 96(10):3747–3754
Lee MH, Cho EJ, Choi ES, Bang MH, Sohn SH (2016b) The effect of hen age on egg quality in commercial layer. Korean J Poult Sci 43(4):253–261
Molnar A, Maertens L, Ampe B, Buyse J, Kempen I, Zoons J, Delezie AE (2016) Changes in egg quality traits during the last phase of production: is there potential for an extended laying cycle? Br Poult Sci 57(6):842–847
Rodríguez-Navarro A, Kalin O, Nys Y, Garcia-Ruiz JM (2002) Influence of the microstructure on the shell strength of eggs laid by hen of different ages. Br Poult Sci 43(3):395–403
Roberts JR, Chousalkar K, Samiullah S (2013) Egg quality and age of laying hens: implications for product safety. Anim Prod Sci 53(12):1291–1297
Kulshreshtha G, Rodriguez-Navarro A, Sanchez-Rodriguez E, Diep T, Hincke MT (2018) Cuticle and pore plug properties in the table egg. Poult Sci 97(4):1382–1390
Hudson BP, Dozier Iii WA, Wilson JL, Sander JE, Ward TL (2004) Reproductive performance and immune status of caged broiler breeder hens provided diets supplemented with either inorganic or organic sources of zinc from hatching to 65 wk of age. J Appl Poult Res 13(2):349–359
Zhang YN, Zhang HJ, Wang J, Yue HY, Qi XL, Wu SG, Qi GH (2017) Effect of dietary supplementation of organic or inorganic zinc on carbonic anhydrase activity in eggshell formation and quality of aged laying hens. Poult Sci 96(7):2176–2183
Saleh AA, Eltantawy MS, Gawish EM, Younis HH, Amber KA, El AE, Ebeid TA (2019) Impact of dietary organic mineral supplementation on reproductive performance, egg quality characteristics, lipid oxidation, ovarian follicular development, and immune response in laying hens under high ambient temperature. Biol Trace Elem Res 16:1–9
Tůmová E, Ledvinka AZ (2009) The effect of time of oviposition and age on egg weight, egg components weight and eggshell quality. Arch Geflügelk 73(2):110–115
Butcher GD, Miles R (2002) Subject: concepts of eggshell quality. Available at: http://edis.ifas.ufl.Edu/ VM013. Accessed Sept 2002
Suk YO, Park C (2001) Effect of breed and age of hens on the yolk to albumen ratio in two different genetic stocks. Poult Sci 80(7):855–858
Akyurek H, Okur AA (2009) Effect of storage time, temperature and hen age on egg quality in free-range layer hens. J Anim Vet Adv 8(10):1953–1958
Sun Q, Guo Y, Li J, Zhang T, Wen J (2010) Effects of methionine hydroxy analog chelated Cu/Mn/Zn on laying performance, egg quality, enzyme activity and mineral retention of laying hens. J Poult Sci 49(1):20–25
Li LL, Gong YJ, Zhan HQ, Zheng YX, Zou XT (2018) Effects of dietary Zn-methionine supplementation on the laying performance, egg quality, antioxidant capacity, and serum parameters of laying hens. Poult Sci 98(2):923–931
Silversides FG, Twizeyimana F, Villeneuve P (1993) Research note: a study relating to the validity of the Haugh unit correction for egg weight in fresh eggs. Poult Sci 72(4):760–764
Cunningham FE, Cotterill OJ, Funk EM (1960) The effect season and age of bird on egg size, quality and yield. Poult Sci 39(2):300–308
Bell DD, Weaver WD (2002) Commercial chicken meat and egg production, 5th edn. Kluwer Academic Publishers, New York
Rodríguez-Navarro AB, Domínguez-Gasca N, Muñoz A, Ortega-Huertas M (2013) Change in the chicken eggshell cuticle with hen age and egg freshness. Poult Sci 92(11):3026–3035
Johnston SA, Gous RM (2007) Modelling the changes in the proportions of the egg components during a laying cycle. Br Poult Sci 48(3):347–353
Skrivan M, Skrivanova V, Marounek M (2005) Effect dietary zinc, iron and copper in layer feed on distribution of these elements in eggs, liver excreta, soil and herbage. Poult Sci 84:1570–1575
Plaimast H, Sirchakwal P, Puwastien P, Kijparkorn S (2008) Effect of supplementary zinc form organic sources on zinc deposition in eggs and laying performance. Thai J Vet Med 38(3):47–53
Sefton AE, Leeson S (2004) Response of broilers fed organic vs. inorganic trace minerals. Int Poult Sci forum in abstracts (p. 1774)
Kim WK, Patterson PH (2005) Effects of dietary zinc supplementation on hen performance, ammonia volatilization, and nitrogen retention in manure. J Environ Sci Health B 40(4):675–686
Ammerman CB, Baker DH, Lewis AJ (1995) Introduction to bioavailability of nutrients for animals. In: Ammerman CB, Baker DH, Lewis AJ (eds) Bioavailability of nutrients for animals: amino acids, minerals, and vitamins, 1st edn. Academic Press, San Diego
Richards JD, Fisher PM, Evans JL, Wedekind KJ (2015) Greater bioavailability of chelated compared with inorganic zinc in broiler chicks in the presence or absence of elevated calcium and phosphorus. Open Access Anim Physiol 7:97
Funding
The authors are grateful to the Iranian National Science Foundation (INSF) for research funding support. This study was funded by grant number (95835756).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Behjatian Esfahani, M., Moravej, H., Ghaffarzadeh, M. et al. Comparison the Zn-Threonine, Zn-Methionine, and Zn Oxide on Performance, Egg Quality, Zn Bioavailability, and Zn Content in Egg and Excreta of Laying Hens. Biol Trace Elem Res 199, 292–304 (2021). https://doi.org/10.1007/s12011-020-02141-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12011-020-02141-8