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Effects of Fluoride on Autophagy in Mouse Sertoli Cells

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Abstract

Fluoride had been reported to damage the structure and function of testicular tissues and reproductive cells; however, the mechanisms underlying its toxicity remained unclear. Autophagy plays a key role in reproductive function. In this study, we aimed to investigate the effect of fluoride on autophagy in Sertoli cells. Sertoli cells were exposed to 0, 0.125, 0.25, and 0.5 mM NaF for 24 h. The results showed that fluoride exposure up-regulated Beclin1 and p62 mRNA and protein expression levels with concomitant down-regulated mRNA and protein expression levels of LC3 and Atg5. In conclusion, exposure to fluoride impaired the autophagy process in Sertoli cells, which could be one of fluoride’s mechanisms in male reproductive toxicity.

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Funding

This work was supported by the National Natural Science Foundation (Grant No. 31741120, 30172380) of China and the Research Project is supported by the Shanxi Scholarship Council of China (Grant No. 2017-071).

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Authors and Affiliations

  1. Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People’s Republic of China

    Zhiyuan Feng, Chen Liang, Ram Kumar Manthari & Jianhai Zhang

  2. Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang, 712046, Shaanxi, People’s Republic of China

    Chong Wang

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  1. Zhiyuan Feng
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  4. Chong Wang
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Correspondence to Jianhai Zhang.

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All regulations of the Institutional Animal Care and Use Committee of Shanxi Agricultural University were strictly followed during the experiment to protect the welfare of the animals.

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The authors declare that they have no conflicts of interest.

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Feng, Z., Liang, C., Manthari, R.K. et al. Effects of Fluoride on Autophagy in Mouse Sertoli Cells. Biol Trace Elem Res 187, 499–505 (2019). https://doi.org/10.1007/s12011-018-1405-z

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