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NDRG2 Promotes Lens Epithelial Cells Senescence via NLRP3/Caspase1-Mediated Pyroptosis

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Abstract

Object

This study aims to investigate the molecular mechanism of NDRG2 (N-myc downstream-regulated gene 2) in the cell senescence of lens endothelial cells.

Methods

Lens endothelial cells (SRA01/04) were irradiated with UVB at different times. Cell viability was measured by CCK-8 kit and cell cycle was detected by flow cytometry. Cell senescence was detected using SA-β-gal staining. Western blot was utilized to detect the expressions of p53, p21 and NDRG2. TUNEL staining and flow cytometry were used to detect apoptosis and pyroptosis.

Results

UVB-irradiation significantly induces cell senescence and the expression of NDRG2, p53 and p21 in SRA01/04 cells was up-regulated. Down-regulation of NDRG2 inhibited UVB-induced cell senescence, significantly reversed pyroptosis and promoted cell proliferation. UVB-induced pyroptosis is closely related to caspase-1/NLRP3 axis.

Conclusion

Our study confirmed downregulation of NDRG2 significantly inhibited UVB radiation-induced cell senescence by regulating caspase-1/NLRP3-mediated pyroptosis.

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Data Availability

All data in the article will be made available by the authors, without undue reservation.

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Acknowledgements

We are grateful to all participants for their contributions to the present study.

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Authors and Affiliations

  1. The Second Department of Ophthalmology, Cangzhou Central Hospital, Building 20, East District, Yunhe New Town, Cangzhou, 061000, Hebei Province, China

    Huilan Zhang

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  1. Huilan Zhang
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H. Z.: Writing, Conceptualization, Methodology, Software, Reviewing.

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Correspondence to Huilan Zhang.

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Zhang, H. NDRG2 Promotes Lens Epithelial Cells Senescence via NLRP3/Caspase1-Mediated Pyroptosis. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04801-6

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