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A Pilot Urinary Proteome Study Reveals Widespread Influences of Circadian Rhythm Disruption by Sleep Deprivation

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Abstract

It is widely accepted that circadian rhythm disruption caused short- or long-term adverse effects on health. Although many previous studies have focused on exploration of the molecular mechanisms, there is no rapid, convenient, and non-invasive method to reveal the influence on health after circadian rhythm disruption. Here, we performed a high-resolution mass spectrometry-based data-independent acquisition (DIA) quantitative urinary proteomic approach in order to explore whether urine could reveal stress changes to those brought about by circadian rhythm disruption after sleep deprivation. After sleep deprivation, the subjects showed a significant increase in both systolic and diastolic blood pressure compared with routine sleep. More than 2000 proteins were quantified and they contained specific proteins for various organs throughout the body. And a total of 177 significantly up-regulated proteins and 68 significantly down-regulated proteins were obtained after sleep deprivation. These differentially expressed proteins (DEPs) were associated with multiple organs and pathways, which reflected widespread influences of sleep deprivation. Besides, machine learning identified a panel of five DEPs (CD300A, SCAMP3, TXN2, EFEMP1, and MYH11) that can effectively discriminate circadian rhythm disruption. Taken together, our results validate the value of urinary proteome in predicting and diagnosing the changes by circadian rhythm disruption.

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Data Availability

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the iProX [69] partner repository with the dataset identifier PXD038035.

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Funding

This study was supported by the National Natural Science Foundation (32171431).

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Author notes

  1. Li Zhou, Xinyu Lu, and Xiaoling Wang contributed equally to this work and they are regarded as co-first authors.  

Authors and Affiliations

  1. Department of Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China

    Li Zhou, Xiaoling Wang, Liyuan Meng, Qin Fu, Li Xia & Shuang Meng

  2. Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China

    Xinyu Lu, Zhixi Huang, Yunzhe Wu & Liyang Zhou

Authors
  1. Li Zhou
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  2. Xinyu Lu
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  3. Xiaoling Wang
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  6. Liyang Zhou
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  7. Liyuan Meng
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Contributions

Conceptualization: LX. Methodology: XL, LM, and QF. Formal analysis: LZ, LX, and SM. Investigation: XL, ZH, YW, and LZ. Data curation: LZ, XW, LX, and SM. Writing—original draft preparation: LZ and SM. Writing—review and editing: LX, XW, and SM. Funding acquisition: LX and XL.

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Correspondence to Li Xia or Shuang Meng.

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This study had been approved by the ethics committee of Shanghai Jiao Tong University School of Medicine with No. 2022-A-05.

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Zhou, L., Lu, X., Wang, X. et al. A Pilot Urinary Proteome Study Reveals Widespread Influences of Circadian Rhythm Disruption by Sleep Deprivation. Appl Biochem Biotechnol 196, 1992–2011 (2024). https://doi.org/10.1007/s12010-023-04666-9

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