Abstract
Colon cancer is the most prevalent cancer and causes the highest cancer-associated mortality in both men and women globally. It has a high incidence and fatality rate, which places a significant burden on the healthcare system. The current work was performed to understand the beneficial roles of nerolidol on the viability and cytotoxic mechanisms in the colon cancer HCT-116 cells. The MTT cytotoxicity assay was done to investigate the effect of nerolidol at different doses (5–100 µM) on the HCT-116 cell viability. The impacts of nerolidol on ROS accumulation and apoptosis were investigated using DCFH-DA, DAPI, and dual staining assays, respectively. The flow cytometry analysis was performed to study the influence of nerolidol on the cell cycle arrest in the HCT-116 cells. The outcomes of the MTT assay demonstrated that nerolidol at different doses (5–100 µM) substantially inhibited the HCT-116 cell viability with an IC50 level of 25 µM. The treatment with nerolidol appreciably boosted the ROS level in the HCT-116 cells. The findings of DAPI and dual staining revealed higher apoptotic incidences in the nerolidol-exposed HCT-116 cells, which supports its ability to stimulate apoptosis. The flow cytometry analysis demonstrated the considerable inhibition in cell cycle at the G0/G1 phase in the nerolidol-exposed HCT-116 cells. Our research showed that nerolidol can inhibit the cell cycle, increase ROS accumulation, and activate apoptosis in HCT-116 cells. In light of this, it may prove to be a potent and salutary candidate to treat colon cancer.
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Abbreviations
- DMOS:
-
Dimethyl sulfoxide
- FBS:
-
Fetal bovine serum
- ROS:
-
Reactive oxygen species
- DAPI:
-
4′,6-Diamidino-2-phenylindole
- DCFH-DA:
-
2′-7′-Dichlorodihydrofluorescein diacetate
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This project was supported by Researchers Supporting Project number (RSP2023R383), King Saud University, Riyadh, Saudi Arabia.
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Zhao, X., Chinnathambi, A., Alharbi, S.A. et al. Nerolidol, Bioactive Compound Suppress Growth of HCT-116 Colorectal Cancer Cells Through Cell Cycle Arrest and Induction of Apoptosis. Appl Biochem Biotechnol 196, 1365–1375 (2024). https://doi.org/10.1007/s12010-023-04612-9
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DOI: https://doi.org/10.1007/s12010-023-04612-9