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Novel Competitive Chemiluminescence DNA Assay Based on Fe3O4@SiO2@Au-Functionalized Magnetic Nanoparticles for Sensitive Detection of p53 Tumor Suppressor Gene

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Abstract

A simple, rapid response time and ultrahigh sensitive chemiluminescence (CL) DNA assay based on Fe3O4@SiO2@Au-functionalized magnetic nanoparticles (Au-MNPs) was developed for detection of p53 tumor suppressor gene. In this study, 2′,6′-dimethylcarbonylphenyl-10-sulfopropyl acridinium-9-carboxylate 4′-NHS ester (NSP-DMAE-NHS), as a new kind of highly efficient luminescence reagent, was immobilized on the complementary sequence of the wild-type p53 (ssDNA) to improve the detection sensitivity. The optimal concentration of ssDNA-(NSP-DMAE-NHS) conjugates mixed with the wild-type p53 (wtp53) samples respectively. Then, the wtp53-Au-MNPs conjugates were added to continue the competitive reaction in the above solution. Subsequently, the Au-MNPs separated under magnetic field, measured by a homemade luminescent measurement system. Under optimal conditions, the method exhibited ultrasensitive sensitivity with a detection limit of 0.001 ng mL−1 (0.16 pM), a wide range of liner response from 0.001 ng mL−1~6.6 μg mL−1. Therefore, the immunomagnetic nanocomposites-based detection strategy was rapid, low-cost, and highly sensitive that can be easily extended to the early diagnosis of cancer development and monitoring of patient therapy.

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Funding

This work was supported by the National Natural Science Foundation of China (NSFC, Grant No. 81371642) and the 111 Project (B14040).

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Authors and Affiliations

  1. Electronic Materials Research Laboratory, Key Laboratory of the Ministry of Education & International Center for Dielectric Researh, Xi’an Jiaotong University, Xi’an, 710049, People’s Republic of China

    Linyu Wang & Xi Yao

  2. Tongji University, Shanghai, People’s Republic of China

    Manwen Yao

  3. School of Science, Xi’an Jiaotong University, Xi’an, People’s Republic of China

    Xiangyi Fang

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  1. Linyu Wang
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  2. Manwen Yao
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  3. Xiangyi Fang
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  4. Xi Yao
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Correspondence to Linyu Wang.

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Wang, L., Yao, M., Fang, X. et al. Novel Competitive Chemiluminescence DNA Assay Based on Fe3O4@SiO2@Au-Functionalized Magnetic Nanoparticles for Sensitive Detection of p53 Tumor Suppressor Gene. Appl Biochem Biotechnol 187, 152–162 (2019). https://doi.org/10.1007/s12010-018-2808-1

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