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Long non-coding RNA Plasmacytoma variant 1 in acute ischemic stroke: association with disease risk, severity, and recurrence-free survival and relation with inflammatory cytokines

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Abstract

Objective

Long non-coding RNA plasmacytoma variant 1 (Lnc-PVT1) is implied with neuron apoptosis, inflammatory cytokines recruitment, endothelial cell proliferation, and angiogenesis; the latter are closely implicated in acute ischemic stroke (AIS) pathology. However, clinical significance of Lnc-PVT1 in AIS management remains unexplored. Thus, this study aimed to investigate this topic.

Methods

In total, 110 patients AIS and 110 controls were enrolled. Peripheral blood mononuclear cells (PBMCs) and serum were extracted from AIS patients and controls. Then, RT-qPCR was performed to determine the PBMC Lnc-PVT1 expression in AIS patients and controls. Besides, we used ELISA to evaluate serum interferon gamma (INF-γ), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and IL-17A in AIS patients. Additionally, in terms of recurrence-free survival (RFS) analysis, the Lnc-PVT1 expression was classified from quantile 1 to quantile 4 regarding the Lnc-PVT1 expression in AIS patients.

Results

AIS patients had higher Lnc-PVT1 expression compared with that in controls (P < 0.001) with a good supplementary diagnostic value for AIS (area under the curve (AUC) = 0.916 (95%CI 0.881–0.951). Furthermore, Lnc-PVT1 expression was positively linked with NIHSS score (r = 0.380, P < 0.001), IFN-γ (r = 0.217, P = 0.023), TNF-α (r = 0.311, P = 0.001), IL-6 (r = 0.235, P = 0.014), and IL-17A (r = 0.253, P = 0.008), separately. RFS of AIS patients with Lnc-PVT1 quantile 1–2 was higher than that of patients with Lnc-PVT1 quantile 3–4 somehow (P = 0.050).

Conclusion

Lnc-PVT1 not only correlates with AIS risk, inflammation, and disease severity, but also reveals a dependable value for AIS prognostication, which still needs further studies for validation.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This study was supported by the National Key R&D Program of China (2017YFE0103700) and Natural Science Foundation of China (82071300).

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Author notes

  1. Liqiang Yu and Weihua Ling contributed equally to this work.

Authors and Affiliations

  1. Department of Neurology, The First Affiliated Hospital of Soochow University, Suzhou, China

    Liqiang Yu & Qi Fang

  2. Department of Emergency, The First Affiliated Hospital of Soochow University, Suzhou, China

    Weihua Ling

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  1. Liqiang Yu
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  2. Weihua Ling
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Correspondence to Qi Fang.

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This study was conducted with the approval from the Ethics Committee of The First Affiliated Hospital of Soochow University.

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Yu, L., Ling, W. & Fang, Q. Long non-coding RNA Plasmacytoma variant 1 in acute ischemic stroke: association with disease risk, severity, and recurrence-free survival and relation with inflammatory cytokines. Ir J Med Sci 191, 1863–1869 (2022). https://doi.org/10.1007/s11845-021-02724-x

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  • DOI: https://doi.org/10.1007/s11845-021-02724-x

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