Abstract
The ATP-binding cassette (ABC) transporter family is one of the largest protein families in plants and plays an essential role in addressing biotic and abiotic stresses. Wheat, a vital global grain crop, faces multifaceted safety challenges, primarily from fungal diseases like stripe rust and powdery mildew. In the present study, we identified the whole genome of the wheat ABC family, and 463 nonredundant ABC genes were identified. The ABC family can be divided into nine evolutionary branches and eight subfamilies based on phylogenetic tree analysis. This paper delved deeper into characterizing the gene structure, promoter region, and gene expression within the TaABC family. Segmental duplication was the main reason for the expansion of the TaABC genes. Ka/Ks analysis suggested that most TaABC genes were intensely purified and selected. The collinear analysis of TaABC and other species showed that the ABC genes were conserved in evolution. RNA-seq data and qPCR data from wheat infected with powdery mildew or stripe rust showed that most TaABC genes were induced to change expression. The candidate genes TaABCB15-3B and TaABCG38 exhibited responsiveness to powdery mildew in resistant/susceptible wheat, while remaining unresponsive to stripe rust. Our findings serve as a valuable reference for gaining a deeper understanding of the function and evolution of TaABCs, aiding in the identification of enduring disease resistance genes within the TaABCs of wheat.
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Data availability
NCBI publicly available datasets were analyzed in this study. These data can be found here PRJNA243835, PRJNA387101, PRJNA472848, PRJNA613349, PRJNA637808, and PRJNA401295. All data generated or analyzed during this study are included in this published article and the additional files.
Abbreviations
- Pst :
-
Puccinia striiformis F. sp. tritici Eriks
- Bgt :
-
Blumeria graminis F. sp. tritici
- ABC:
-
ATP-binding cassette
- MeJA:
-
Methyl jasmonate
- ABA:
-
Abscisic acid
- MYB:
-
Myeloblastosis
- TMDs:
-
Transmembrane domains
- NBDs:
-
Nucleotide-binding domains
- Ka:
-
The non-synonymous substitution rate
- Ks:
-
The synonymous substitution rate
- Ta:
-
Triticum aestivum L.
- DON:
-
Deoxynivalenol
- JA:
-
Jasmonic acid
- PEN:
-
PENETRATION
- MYA:
-
Millions years ago
- FPKM:
-
Fragments per kilobase of exon model per million mapped fragments
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Acknowledgements
The authors would like to thank Guorong Wei, Senior laboratory Technician of Plant Protection College of Northwest A&F University, for providing us with stripe rust fungus.
Funding
This research was funded by the Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement Program of China (No. Z100021811), National Natural Science Foundation of China (31971741) and the Shaanxi Innovation Team Project (2018TD-004), Germplasm Innovation and Breakthrough Cultivar Breeding of Wheat.
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Conceptualization: WJ and HZ; methodology: GW; software: GW and DL; validation: WJ, CC and HZ; formal analysis: GW and XZ; investigation: GW, XZ and CZ; resources: CC; data curation: GW; writing—original draft preparation: GW; writing—review and editing: JG; visualization: GW and JG; supervision: HZ; project administration: CC; funding acquisition: WJ and CC. All authors have read and agreed to the published version of the manuscript.
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Wang, G., Gu, J., Long, D. et al. Genome-wide identification of wheat ABC gene family and expression in response to fungal stress treatment. Plant Biotechnol Rep (2023). https://doi.org/10.1007/s11816-023-00881-2
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DOI: https://doi.org/10.1007/s11816-023-00881-2