Skip to main content
SpringerLink
Log in

GC/MS analysis and anti-inflammatory effect of leaf extracts from Hibiscus syriacus through inhibition of NF-κB and MAPKs signaling in LPS-stimulated RAW264.7 macrophages

  • Original Article
  • Published:
Plant Biotechnology Reports Aims and scope Submit manuscript

A Correction to this article was published on 25 June 2020

This article has been updated

Abstract

Hibiscus syriacus (H. syriacus), which belongs to Malvaceae, is a perennial herb widely distributed in East Asia. In this study, we investigated the anti-inflammatory effects and its potential molecular mechanisms of leaves extracted with 70% ethanol of H. syriacus (HS-L) at the mRNA and protein level when RAW264.7 macrophages were stimulated by LPS. HS-L dose dependently (0, 25, 50, 100 μg/mL) reduced NO production by inhibiting iNOS, COX-2, and IL-β expression in LPS-induced RAW264.7 macrophages. HS-L decreased the LPS-stimulated degradation and phosphorylation of IkB-a. Moreover, treatment with HS-L suppressed LPS-induced DNA binding of NF-kB and nuclear translocation of p65. Furthermore, HS-L suppressed the phosphorylation of ERK1/2 and p38. HS-L was analyzed to contain compounds such as 2-pentadecanone, methyl palmitate, and ethyl palmitate in GC/MS analysis. These results suggested that HS-L could utilize anti-inflammatory activity by inhibiting NF-κB and MAPKs signaling pathways and that HS-L could be used as a natural anti-inflammatory agent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Change history

  • 25 June 2020

    In the original publication of the article, the name of third author was published incorrectly as “Som Da Kim”. The correct name is “Da Som Kim”.

References

  • Ahn SK, Goo YM, Ko KH, Lee SJ, Moon YH, Lee SS, Kim JW, Lee SS (2014) Study on the evaluation of nutritional values and antioxidant activities for herbal medicine by-products. J Agriculture Life Sci 48:101–110

    Article  Google Scholar 

  • Choi DH, Cho UM, Hwang HS (2018) Anti-inflammation effect of rebaudioside A by inhibition of the MAPK and NF-κB signal pathway in RAW264.7 macrophage. J Appl Biol Chem 61:205–211

    Article  Google Scholar 

  • El-Demerdash E (2011) Anti-inflammatory and antifibrotic effects of methyl palmitate. Toxicol Appl Pharmacol 254:238–244

    Article  CAS  Google Scholar 

  • Hsu RJ, Hsu YC, Chen SP, Fu CL, Yu JC, Chang FW, Chen YH, Liu JM, Ho JY, Yu CP (2015) The triterpenoids of Hibiscus syriacus induce apoptosis and inhibit cell migration in breast cancer cells. BMC Complement Altern Med 15:65–74

    Article  Google Scholar 

  • Jang AR (2012) A study on cosmetic physiological and skin cell activity of Hibiscus syriacus extracts. M. S Thesis. Hannam University Daejeon Kor.

  • Kaminska B (2005) MAPK signalling pathways as molecular targets for anti-inflammatory therapy from molecular mechanisms to therapeutic benefits. Biochem Biophys Acta 1754:253–262

    CAS  PubMed  Google Scholar 

  • Kim EK, Choi EJ (2010) Pathological roles of MAPK signaling pathways in human diseases. Biochem Biophys Acta 1802:396–405

    CAS  PubMed  Google Scholar 

  • Kim HN, Park SB, Park GH, Eo HJ, Song JH, Kwon HY, Jeong JB (2018) Anti-inflammatory effect of the root extracts from Hibiscus syriacus in LPS-stimulated RAW264.7 cells. Korean J Plant Res 31:211–217

    Google Scholar 

  • Kim HK (2014) Role of ERK/MAPK signalling pathway in anti-inflammatory effects of Ecklonia cava in activated human mast cell line-1 cells. Asian Pacific J Tropical Med 7:703–708

    Article  CAS  Google Scholar 

  • Kim JB, Han AR, Park EY, Kim JY, Cho W, Lee J, Seo EK, Lee KT (2007) Inhibition of LPS-induced iNOS, COX-2 and cytokines expression by poncirin through the NF-k B inactivation in RAW 264.7 macrophage cells. Biol Pharm Bull 30:2345–2351

    Article  CAS  Google Scholar 

  • Kim KH, Ko KI, Kang EJ, Yang EK, Park SN (2004) A research trend of natural product on well-being industry. J Soc Cosmet Sci Korea 30:239–343

    CAS  Google Scholar 

  • Kim MJ, Kim KBWR, Park SH, Choi JS, Ahn DH (2017) Anti-inflammatory effect of Chondria crassicaulis ethanol extract on MAPKs and NF-κB signaling pathway in LPS-induced RAW 264.7 macrophages. Korean Soc Biotechnol Bioeng J 32:352–360

    Google Scholar 

  • Lee ST, Jeong YR, Ha MH, Kim SH, Byun MW, Jo SK (2000) Induction of nitric oxide and TNF-α by herbal plant extracts in mouse macrophages. J Korean Soc Food Sci Nutr 29:342–348

    Google Scholar 

  • Liu T, Zhang L, Joo D, Sun SC (2017) NF-κB signaling in inflammation. Signal Trans Targeted Therapy 2:e17023

    Article  Google Scholar 

  • Lu J, Fang K, Wan S, Xiong L, Zhang C, Liu Z, Guan X, Zheng R, Wang G, Zheng J, Wang F (2018) Anti-inflammatory effect of columbianetin on lipopolysaccharide-stimulated human peripheral blood mononuclear cells. Mediators Inflamm 2018:1–8

    Google Scholar 

  • Morrison DK, Davis RJ (2003) Regulation of MAP kinase signaling modules by scaffold proteins in mammals. Annu Rev Cell Dev Biol 19:91–118

    Article  CAS  Google Scholar 

  • Park SB, Song HM, Kim HN, Park GH, Son HJ, Um Y, Park JA, Jeong JB (2018) Anti-inflammatory effect of biji (soybean curd residue) on LPS-stimulated RAW264.7 cells. Korean J Plant Res 31:117–123

    Google Scholar 

  • Raduan SZ, Abdul Aziz MWH, Roslida AH, Zakaria ZA, Zuraini A, Hakim MN (2013) Anti- inflammatory effects of Hibiscus rosa-sinensis L. and Hibiscus rosa-sinensis var. alba ethanol extracts. Int J Pharmacy Pharmaceutical Sci 5:754–762

    Google Scholar 

  • Saeed NM, El-Demerdash E, Abdel-Rahman HM, Algandaby MM, Al-Abbasi FA, Abdel-Naim AB (2012) Toxicol Appl Pharmacol 264:84–93

    Article  CAS  Google Scholar 

  • Siyumbwa SN, Ekeuku SO, Amini F, Emerald NM, Sharma D, Okechukwu PN (2019) Wound healing and antibacterial activities of 2-Pentadecanone in streptozotocin-induced Type 2 diabetic rats. Phcog mag 15:71–77

    CAS  Google Scholar 

  • Tak PP, Firestein GS (2001) NF-kB: a key role in inflammatory diseases. J Clin Invest 107:7–11

    Article  CAS  Google Scholar 

  • Whitmarsh AJ (2006) The J.I.P. family of MAPK scaffold proteins. Biochem Soc Trans 34:828–832

    Article  CAS  Google Scholar 

  • Woo HS, Lee SM, Heo JD, Lee MS, Kim YS, Kim DW (2018) Anti-inflammatory activity of extracts of Hovenia dulcis on lipopolysaccharides-stimulated RAW264.7 cells Korean. J Plant Res 31:466–477

    Google Scholar 

  • Youn HS (2012) The anti-inflammatory effects of phytochemicals by the modulation of innate immunity. J Exp Biomed Sci 18:181–192

    Google Scholar 

  • Zhang C, Li C, Jia X, Wang K, Tu Y, Wang R, Liu K, Lu T, He C (2019) In vitro and in vivo anti-inflammatory effects of polyphyllin VII through downregulating MAPK and NF-B pathways. Molecules 24:1–17

    Google Scholar 

Download references

Acknowledgements

This work was supported by a grant from National Institute of Forest Science in 2020 (project number: FG0403-2018-01-2020).

Author information

Authors and Affiliations

  1. Forest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju, 36040, Korea

    Hyun Ji Eo, Som Da Kim, Yeongyeong Kang, Youngki Park & Gwang Hun Park

  2. Forest Tree Improvement Division, Forest Bioresources Department, National Institute of Forest Science, Suwon, 16631, Korea

    Hae Yun Kwon

Authors
  1. Hyun Ji Eo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hae Yun Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Som Da Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yeongyeong Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Youngki Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Gwang Hun Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gwang Hun Park.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Eo, H.J., Kwon, H.Y., Da Kim, S. et al. GC/MS analysis and anti-inflammatory effect of leaf extracts from Hibiscus syriacus through inhibition of NF-κB and MAPKs signaling in LPS-stimulated RAW264.7 macrophages. Plant Biotechnol Rep 14, 539–546 (2020). https://doi.org/10.1007/s11816-020-00628-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11816-020-00628-3

Keywords

Search

Navigation