Italobdella bieleckii sp. n. (Hirudinea: Piscicolidae), a new leech species from the Danube in Slovakia

Notwithstanding the genus Italobdella has been hypothesized to have doubtful taxonomic value, we decided to update its diagnosis by describing the new species – Italobdella bieleckii sp. n. The leeches were collected from the stony bottom along the shore of the Danube River in Slovakia. In the study, we implemented the model of leech body form and the set of external and internal morphological characters to present the similarity of the new species to the other fish leeches. As a result, we were able to construct the key for determining leeches belonging to the genus Italobdella hoping that the species could be found and determined by other researchers. This would enrich our knowledge about the actual distribution of these leeches and strengthen their systematic position in the genus.

Leeches of the genus Italobdella have a reproductive system organized similarly as in marine leeches of the monotype genus Galatheabdella Richardson et Meyer, 1973(Sawyer 1986Bielecki 1993Bielecki , 1997Bielecki et al. 2012Bielecki et al. , 2013. They possess a vector tissue located before the opening of the oviduct, but conducting strands of vector tissue connect the tissue with ovisacs from one side and the posterior part of the copulatory bursa of atrium from the other side. The connection between conducting strands of vector tissue with the posterior part of atrium is lacking in the reproductive system of Italobdella spp. (Bielecki 1993;Bielecki et al. 2008).
The study includes a diligent description of the body form, external and internal morphology and the key for the identification of leeches belonging to the genus Italobdella.
The aim of the study was a diligent morphological analysis of the body form and other external and internal morphological characters of the piscicolid leech found in Slovakia. Based on the results, the new leech species was described and classified within the genus Italobdella Bielecki, 1993 and the diagnosis of the genus was updated.

Materials and methods
Material examined during this study was collected in Slovakia: the Danube, Kravany (Čenkov) (river kilometer 1733), 1.10.1992, one specimen collected by V. Košel; the Danube, Štúrovo, (river kilometer 1720), 17.10.1992, three specimens including one with a spermatophore collected by V. Košel; the Danube, mixed sample, six leeches: one specimen collected by E. Elexová.
The holotype and the paratype, as well as the other three collected specimens were preserved in 4% formalin (Košel leg., 1.10. 1992(Košel leg., 1.10. , 17.10. 1992. The holotype (no. MIZ 1/2021/1) and paratype (no. MIZ 1/2021/2) of the new Italobdella species have been deposited at the Museum of Zoology of the Polish Academy of Sciences in Warsaw. The other material is in the first author's collection.
All specimens were measured according to the model of the leech body by Bielecki (1997) (Table 1). Detailed dissections were performed on all specimens according to the procedure by Bielecki (1997). Leeches were dissected on their dorsal side under the stereomicroscope OLYMPUS SZ-ST. Using a scalpel, several incisions of the integument were performed along the body midline, starting from the anterior sucker to the caudal sucker. Portions of the integument were spread aside using dissection needles to immobilize the specimen and reveal the alimentary tract. It is usually covered with various tissues (like muscles, coelomic structures and fat cell groups), which should be removed to interpret the tract structure better. In the next step, the structures of the alimentary tract should be resected to uncover the reproductive system. Bearing in mind that the area is very small, and the reproductive organs are very fragile, one should be extremely careful while taking out portions of the alimentary tract. After this is accomplished, it is possible to observe the positions of particular reproductive system structures and orient them in reference to ganglia.

Construction, description and application of the model
The model (Bielecki and Epshtein 1994;Cichocka and Bielecki 2015) presents the leech body on a plane, as two ellipses (suckers) and trapeziums situated between them (anterior body part -trachelosome -two trapeziums; posterior body part -urosome -four trapeziums) (Fig. 1a). Besides, transverse sections through the trachelosome and urosome are considered as two ellipses (Fig. 1b, c). An abundant material provides evidence that the model permits a sufficiently exact description of the body form of various leech species (Bielecki et al. 2012(Bielecki et al. , 2013Cichocka and Bielecki 2015).
The model is constructed according to the following parameters: (1-4) Parameters describing the form of the anterior sucker: C 1 = vertical diameter; C 1 1 = horizontal diameter; R 1 = length of anterior part of sucker; M 1 = length of posterior part of sucker.
(26-29) Parameters describing the form of posterior sucker: C 1 2 = horizontal diameter; C 2 = vertical diameter; M 2 = length of anterior part of sucker; R 2 = length of posterior part of sucker. The body shape and size are illustrated in Fig. 2 and Tables 1 and 2. The 19 body proportion indices (invariants) are: 1. Index describing L/D 2 = relative body length. Indices describing anterior sucker: 2. C 1 1 /d 1 = ratio of horizontal diameter of sucker to trachelosome width at sucker junction; 3. C 1 1 /D 1 = ratio of horizontal diameter of sucker to greatest width of trachelosome; 4. R 1 / M 1 = length ratio of the anterior part of sucker to its posterior part; 5. C 1 1 /C 1 = ratio of horizontal diameter of sucker to its vertical diameter. Indices describing trachelosome: 6. L 1 /D 1 = ratio of trachelosome length to its greatest width; 7. D 1 /N 1 = ratio of greatest trachelosome width to its greatest height; 8. S 1 /S 2 = ratio of the height of first trapezium to the height of second trapezium. Indices describing urosome: 9. L 2 /D 2 = ratio of urosome length to its greatest breadth; 10. D 2 /N 2 = ratio of greatest urosome width to its greatest height; 11. K 1 / K 2 = ratio of the distance from d 3 to D 2 to the distance from D 2 to d 7 . Indices describing posterior sucker: 12. C 1 2 /d 7 = ratio of horizontal diameter of sucker to urosome width at sucker junction; 13. C 1 2 /D 2 = ratio of horizontal diameter of sucker to greatest body height; 14. R 2 /M 2 = length ratio of the posterior part of sucker to its anterior part; 15. C 1 2 /C 2 = ratio of horizontal diameter of sucker to its vertical diameter. Indices describing relations between urosome and trachelosome: 16. L 2 /L 1 = ratio of urosome length to trachelosome length; 17. D 2 /D 1 = ratio of greatest width of urosome to greatest width of trachelosome; 18. N 2 / N 1 = ratio of greatest height of urosome to greatest height of trachelosome. Index describing proportions of suckers: 19. C 1 2 / C 1 1 = ratio of horizontal diameter of posterior sucker to horizontal diameter of anterior sucker.
The number of specimens examined and the parameters describing their body form are presented in Table 1, and mean values of the body proportion indices are presented in Table 2.
List and description of non-metric 113 character, states are presented in works by Bielecki (1997Bielecki ( , 2001 and Thorp et al. (2019).
The joining or tree clustering algorithm was chosen to interpret the similarity of body form of the 24 piscicolid species. When selecting distance measures, city-block (Manhattan) distance was chosen, which minimizes the effect of more distant objects (morphotypes). Of amalgamation or linkage rules, Ward's method was used since it tends to yield small clusters and with such a low number of species gives a high resolution. The procedure allowed dividing the species morphotypes into two polytypic clusters (Figs. 3 and 4). Statistical analyses and figures were made using STATISTICA 12 (StatSoft, Inc.).

Genus Italobdella Bielecki, 1993
Italobdella Bielecki, 1993: 67-78, Figs. 1-20. Type species: Italobdella ciosi Bielecki, 1993. Diagnosis. Considering the leech diversity in Palearctic, species of the genus Italobdella are most similar to the genus Pawlowskiella Bielecki, 1997, Caspiobdella Epshtein, 1966and Acipenserodbella Epshtein, 1969. In all these taxa spermatheca opening is located anterior to the first pair of respiratory vesicles. The female and male gonopores are well visible on clitellum. Italobdella differs from Pawlowskiella in mid-body somite 3-annulate and 4-annulate (in   Caspiobdella fadejewi (Epshtein, 1961)  Pawlowskiella and Caspiobdella 14-annulate, Acipenesrobdella 6-annulate), spermatheca opening is located between gonopores (Plate I: 4-6) (in Pawlowskiella posterior to the female gonopore), ovaries enter female gonopore posterior to the vector tissue (in Pawlowskiella, Caspiobdella and Acipenserobdella they enter through vector tissue); vector tissue is as a narrow plate transverse to the long body axis, and is located anterior to the oviduct outlet (in Pawlowskiella, Caspiobdella and Acipenserobdella as a circular plate situated on female gonopore). Oviducts of Italobdella do not enter the vector tissue like they do in Pawlowskiella, Caspiobdella and Acipenserobdella. Description. Size very small (5.9 mm) or medium (15.7 mm) and large (21.1 mm). Body short. Division into trachelosome and urosome very well visible, trachelosome flattened and urosome cylindrical. Body smooth, with no papillae. Anterior sucker small or medium-sized, posterior also small and connected somewhat eccentrically. Respiratory vesicles (11 pairs) white, rather small but well visible, often white. Eyes on anterior sucker and eye-like spots on posterior sucker present. Mid-body somite three-annulate with secondary shallower grooves, up to six, or four-annulate with secondary shallower grooves, up to 12. Oesophagus with glands, crop and posterior crop caecum of two equal diverticula, both sides are equal in size or the first is much more developed than the other and it resemble ram horns. Posterior crop caecum incompletely fused; five fenestrae remain at the level of ganglia. Intestine well developed.
Plate I Italobdella bieleckii sp. n. (holotype 1-4): 1 dorsal side (scale bar = 1.73 mm); 2 ventral side (scale bar = 1.73 mm); 3 lateral side (scale bar = 1.73 mm); 4 part with gonopores and spermatheca,male gonopore, SR -opening of spermatheca, -female gonopore, (scale bar = 0.26 mm); 5-6 part with gonopores and spermatheca in Italobdella ciosi (scale bar = 0.43), SEM Spermatheca opening visible as a round white field with an opening in the middle (two annuli posterior to male gonopore). Copulatory area on clitellum small, surrounding spermatheca opening. Prostatic glands on atrium well developed. Ejaculatory ducts small, between fourth and fifth ganglia, or rather slightly protrude beyond fifth ganglion, bent once, with characteristic bulbs. Seminal vesicles reach the second pair of testisacs or are situated between the first and second pairs of testisacs. Vector tissue as a narrow, rectangular plate transverse to body long axis, anterior to oviduct outlet. Oviducts open to female gonopore posterior to vector tissue.

Italobdella bieleckii Cichocka, Košel et Świątek, sp. n.
Plates I (1-4), II-III, Figs. 2a, b, c, 3, 4, Tables 1, 2 Diagnosis. Piscicolinae; freshwater; basically three-annulate; opening of spermatheca (receptaculum seminis) visible as transverse structure to the long body axis located between male and female gonopores (Plate I: 4-6). Fissure of spermatheca is bigger than male gonopore and located two annuli behind it. Female gonopore poorly visible, situated one annulus behind the opening of spermatheca. Copulatory area (CA) on clitellum absent (Plate I: 4). Vector tissue (VT) in the form of narrow plate arranged transverse to the long body axis, anteriorly to the outlet of oviducts. Conducting strands of vector tissue (CS) in the form of quite broad bands connecting ovisacs with VT (Plate III: 2, 6). Oviducts do not enter the VT but they go outside the clitellum below VT (Plate I: 4-6; III: 2, 6).
Italobdella bieleckii sp. n. differs from I. ciosi and I. epshteini in the following characteristics: mid-body somite of three (six) unequal annuli (in I. ciosi and I. epshteini four (12) unequal annuli), nephridiopores clearly visible in middle annuli of somite (in I. ciosi and I. epshteini nephridiopores are not visible), gonopores separated by 4.3 annuli (in I. ciosi and I. epshteini four annuli), two-piece parts of crop and posterior crop caecum unequal, first is significantly bigger than other in form of ram horns (in I. ciosi and I. epshteini two-piece parts of crop and posterior crop caecum are equal), seminal vesicles (VS) twisted many times, before the second pair of testes (T 2 ), (in I. ciosi and I. epshteini classically U shaped, before the first pair of testes (T 1 ) beginning of the ovary).
Description. Size and body form (Figs. 1, 2 and 3; Plate I: 1-3; III: 3-4; Tables 1 and 2). Medium-sized leeches. Of the five specimens, the largest was 15.70 mm long with the greatest width of 3.95 mm, and the smallest was 9.92 mm long with the greatest width of 1.50 mm. Average values were adopted for further analysis of the parameters. The length of the body exceeds the greatest width by 5.2 times. Ratio of the greatest width to the greatest height (thickness) of the urosome is 1.0. Ratio of the greatest width of the urosome to the greatest width of the trachelosome is 1.7. The diameter of the anterior sucker to the greatest width of the trachelosome is 0.9. The ratio of the diameter of the posterior sucker to the largest width of the urosome is 0.7. The diameter of the posterior sucker is slightly more than the diameter of the anterior sucker (1.3 times).
Separation into trachelosome and urosome is visible even in preserved and poorly fed individuals. Body is short, slightly flattened or only trachelosome is flattened and urosome is cylindrical. The anterior sucker is small or medium size, the posterior sucker is also small and connected to the urosome slightly eccentrically (Table 1).
The algorithm of combining or grouping the body forms of 24 Piscicolidae species allowed to divide the morphotypes of the species into two polytypic clusters. Italobdella bieleckii sp. n. was included in cluster I and subcluster I 2 together with I. ciosi as species with the most similar body forms. Italobdella epshteini and Acipenserobdella volgensis (Zykoff, 1903) were in the same subcluster (I 2 ), being external, less similar to these two species (Fig. 3).
Somites. Full somites consist of 3 annulli. Further, shallower dividing furrows are visible on these 3 annuli. The first annulus, the shortest, is divided into two unequal parts, the second (with vesicle), slightly longer than the first, also divided into two unequal parts, and the third, longest, divided into two unequal parts. In total, the somite consists of three annuli separated by deep grooves, and shallower grooves divide annuli further giving finally six annuli in somite (Plate II. 1-2; III. 5).
Body colouration (Plate I: 1-3; III: 3-5). It results from the different arrangement of the two types of melanophores: brown and white. Their composition makes the color of the leeches quite monotonous. A characteristic feature is that both brown and white melanophores do not have protrusions and marked bodies. Even preserved specimens can hardly be confused with the other two species of the genus I. ciosi and I. epshteini. White melanophores on the trachelosome form 4 or 5 spots in the medial plane. To the left and right of the white medial spots, there are also white spots at the same height -paramedical (4 or 5) and on the sides of the body also 4 or 5 white spots. On the urosome, white spots maintain a similar order. There are 14 spots in the medial plane. On the left and right side of the medial spots are two rows of smaller white paramedical spots, with 13 on each side. The coloration, and especially the distribution of white spots on the trachelosome and urosome, resembles that of I. ciosi, but also of Piscicola siddalli Bielecki, Cios, Cichocka and Pakulnicka, 2012. The anterior sucker is uniformly brown. The posterior sucker has very narrow white and wide brown radial streaks. There are 14 of both. Eyes. On the anterior sucker, there are two pairs of eyesthe first one is slightly larger, obliquely to the sagittal plane, the second one is perpendicular and smaller. On the posterior sucker, there are 10 eye-shaped spots located on the edge of faintly defined dark radial streaks (Plate III: 3).
Digestive system (Plate II: 3-6; III: 1). The oral opening is eccentrically located at the posterior part of the anterior sucker and leads back to the proboscis. The position of the proboscis is between the first and third ganglia, starting with the cephalic ganglion mass (ganglia 1-6), what means, the proboscis reaches the ganglion of segment IX. There are salivary glands on the right and left sides of the proboscis. The thin-walled esophagus extends from IX to XII/XIII segment, and it enter the crop (C). There is a diverticulum (pair of pouches) that connects to the esophagus. A characteristic feature of the crop is presence of seven pouches located between the segments, they are paired along the right and left edges, each of them is divided into two parts (crop diverticula -CD). The first pair of diverticula is well developed and shaped like ram's horns (Plate II: 3-6; III: 1). In the XIX/XX somite below the seventh crop diverticulum, the intestine divides into a thin-walled caecum (posterior post caecum PCC) located ventrally, and a thick-walled intestine (I) located dorsally in relation to the previous one (i.e., on the posterior crop). The thin-walled posterior crop (PCC) consists of five diverticula which are not completely merged. There are five openings in its dorsal and ventral parts (at the level of the ganglia). Each diverticulum consists of two parts similar to the crop diverticula (CD) (Plate II: 6). The thickwalled intestine is very elongated with folded edges, so tight that it completely covers the PCC. It has five paired diverticula (ID), of which the first three pairs are well developed, Plate III Italobdella bieleckii sp. n.: 1 alimentary tract of holotype: C -crop, seventh pouches; PCC -posteriori crop (3 pouches, each divided into two diverticula, similarly to the crop (retouched) (scale bar = 0.30 mm); 2 reproductive system of holotype (scale bar = 0.20 mm); 3 dorsal side of paratype (scale bar = 1.77 mm); 4 ventral side of paratype (scale bar = 1.77 mm); 5 part of ventral part with nephridiopores visible (scale bar = 0.22 mm); 6 reproductive system of paratype (scale bar = 0.23 mm); EDejaculatory duct, PG -prostate glands, VT -vector tissue (above the ovisacs), CS -conducting strands of vector tissue, O -ovisac, OV -oviduct, VS -seminal vesicle, T1-T2 -first and second pair of testisacs; 5. S -3-annulate somite, RV -respiratory vesicle, N -nephridiopore directed to the sides and anteriorly. The fourth pair is not much smaller, and its branches are shifted to the sagittal plane. The fifth is very small and hardly visible. Behind it, the intestine forms a twisted tube, which continues into the rectum, ending with the anus (Plate II: 5).
Reproductive system (Plate I: 4; III: 2, 6). There are three openings on the clitellum. The first is a large, clearly visible male gonopore lying exactly in the center of the annulus. Three and a half annuli behind it, there is a second opening -spermatheca, very large, relatively narrow, in the form of a slit transversely arranged to the main axis of the body. The third opening is the female gonopore, small, hardly visible, lying ¾ of the annulus behind the spermatheca. The exact distance between the male and female gonopores is 4.25 annuli. The copulatory area (CA) on the clitellum is usually absent, however, if present, it is very small within the opening of the spermatheca. In the male reproductive system, there are six pairs of testes (T 1 -T 6 ) located between the segments along the section from XIII/XIV segment to the XVIII/XIX segment. Seminal tubules (vasa eferentia) depart from the testicles and connect to the longitudinal vasa deferentia. At the front, the vasa deferentia pass into the seminal vesicles (VS) at the level of the testicles of the second pair (T 2 ). They form the ejaculatory ducts (ED) in the form of thick half loops that do not extend to the fifth ganglion and connect to the general part of the atrium and the sac of the copulatory capsule. In their natural position, the ejaculatory ducts bend in the sagittal plane towards the dorsal side. During dissection the ejaculatory ducts were bent to the sides (by 90°) to better visualise their structure (Plate III: 6). Prostate glands (PG) in the atrium are present. In the female reproductive system, the ovisacs are flattened, half-moon shaped, their posterior ends reach the testisacs of first pair (T 1 ). The anterior ends of the ovisacs (before passing into the oviducts) cover the vector tissue (VT), making the conductive strands of vector tissue (CS) invisible. The oviducts enter the female gonopore posterior to the vector tissue (VT) mass. Italobdella bieleckii sp. n. is characterized by a special structure and location of the vector tissue. It is in the form of a narrow plate arranged transversely to the longitudinal axis of the body and located above the outlet of the oviducts. Due to its location, paired conducting strands of vector tissue (CS) appear in the form of very short, relatively wide strands of fibers connecting each ovisac with the vector tissue. Thus, the space between the converging oviducts and the testisacs of the first pair (6th and 7th ganglia) is free. The vector tissue (VT) and paired conducting strands of VT (CS) are best seen when the ovisacs are exposed posteriorly or to the side by 180° (Plate III: 6).
The qualitative traits phenogram separated 24 Piscicolidae species into two polytypic clusters. Italobdella bieleckii sp. n. is included in cluster II and subcluster II 2 together with Italobdella ciosi and Italobdella epshteini, with I. epshteini being external, less similar to the other two species. Moreover, in the same cluster II but in the subcluster II 1 there were species from the genera Acipenserobdella, Caspiobdella and Pawlowskiella, similar mainly in terms of the reproductive and digestive systems (Fig. 4).
Etymology. Dedicated to Professor Aleksander Bielecki, Polish scientist, with broad scientific interests, with very good methodological skills, which is a unique exampledeeply entering the area of the methodology of natural sciences. The main area of his scientific competence concerns comprehensive research (description, classification, and phylogenetic reconstruction) on leeches. He initiated topics, formulated and tested hypotheses in the field of developmental biology of Trematoda, but most of all he was involved in morphological, faunistic, ethological, hydrobiological, ecological, parasitological, taxonomic, molecular, and systemic research on leeches (Hirudinea), and in leeches parasitizing on fish (Piscicolidae). In his monograph, he described leeches parasitizing on Palearctic fish and solved the hypothesis about the positive phototactic mechanism of finding a host by some of these leeches, formulating the theory of positive phototaxis.

Discussion
According to the systematics based on internal and external morphology, there are 15 genera of fish leeches within the subfamily Piscicolinae Johnston, 1865 distinguished on the basis of the structure of the reproductive system (Caballero 1956;Sawyer 1986;Epshtein 1987Epshtein , 1989Bielecki 1993Bielecki , 1997. However, the status of piscicolid subfamilies has been questioned due to the results of molecular studies in which they appeared to be non-monophyletic (Utevsky and Trontelj 2004). Epshtein (2004) described the leeches from lakes of ancient origin in Asia, Europe and Africa with a particular emphasis on the leeches of the family Piscicolidae. Based on the analyzed distribution of leeches in these lakes, he made a hypothesis about the development and formation of two types of Palearctic leeches from their sea roots: the Eastern type, including the Far East, Baikal, Central Asia, the Eastern Trans-Caucasus and the Western (Caspian) type reaching from the Caspian Sea to the Apennine Peninsula. According to this division, Italobdella bieleckii sp. n. belongs to the Western (Caspian) type. The Caspian Sea (probably around 3 million years old), similarly to Lake Baikal, has an endemic fauna of leeches and is a refuge for the unique leeches of the family Piscicolidae. Some of them, although originally freshwater, are also tolerant of brackish water. From the Caspian Sea and the Danube basin to the Apennines, there is a region where the Caspian fauna occurs, which includes species of genera that differ from the Holarctic and Baikal Piscicolidae in terms of the structure of the female reproductive organs. These genera are as follows: Caspiobdella Epshtein, 1966, Italobdella Bielecki, 1993and Pawlowskiella Bielecki, 1997, with a total of six species: Caspiobdella caspica (Selensky, 1915), C. fadejewi (Epshtein, 1961), C. tuberculata Epshtein, 1966, Italobdella ciosi Bielecki, 1993, I. epshteini Bielecki, 1997 and Pawlowskiella stenosa Bielecki, 1997. Such a result was also obtained in the phenogram concerning the qualitative traits, mainly in terms of the similarity of the characteristics of the reproductive and digestive systems of Piscicolidae. Italobdella bieleckii sp. n. was grouped in cluster II and subcluster II 2 together with I. ciosi and I. epshteini. However, in the same cluster II and subcluster II 1 species from the genera Acipenserobdella, Caspiobdella and Pawlowskiella were assembled (Fig. 4). In the coastal waters of the Caspian Sea, there is Piscicola geometra (Linnaeus, 1761), while in much of the northern part of the basin only Caspiobdella caspica (Selensky, 1915) is present, and the presence of P. geometra was not reported. In the central part of the Caspian Sea, only one species of Piscicolidae has been described, Caspiobdella tuberculata Epshtein, 1966(Epshtein 2013. The occurrence of I. ciosi in Northern Italy is essential for understanding the origin of the Caspian Piscicolidae. This species is believed to be a relic of the waters flowing from the Tethys Sea and inhabits rivers flowing into the Mediterranean, Black and Azov Seas (Epshtein 2004(Epshtein , 2013. Italobdella ciosi is most likely found in the River Pad and its basin, which proves the western border of the Caspian fauna. Species of the genus Piscicola and species of the Caspian type genera, which never had a connection with the sea, after Martinson (1958), we name paleolimnic.
A disputable opinion was presented by Minelli (2006), who believed that I. ciosi was a species of low taxonomic value and was brought to Italy with fish. Unfortunately, he did not present any alternative hypothesis about the origin of this species, not to mention that he did not interpret the reproductive system of the leech. For these reasons, we believe that the approach by Minelli (2006) is within methodological anarchism (Feyerabend 1975;Bielecki et al. 2014). It must be clearly stated that the species Italobdella ciosi, I. epshteini and I. bieleckii sp. n. occur in their natural zone and have a very characteristic reproductive system (Bielecki 1993(Bielecki , 1997Bielecki et al. 2008;Epshtein 2004Epshtein , 2013. Interestingly, Epshtein (2004Epshtein ( , 2013 presented the types of the reproductive system in leeches from the Piscicolidae family that correlate with their distribution. Within these types there is "Caspiobdella" type (subtribe Caspiobdellina), in which Italobdella is included.
Assuming that the distribution of Italobdella species is natural, one can hypothesize that, most likely, they are relics of the waters originated from the Tethys Sea, which inhabited (and still inhabit) the basins of rivers flowing into the Mediterranean Sea, the Black Sea and the Azov Sea. The study by Soes (2010) is significant, as he clearly presented that the collected specimens of C. respirans from Italy (at his disposal) were mistakenly identified, and most likely, they were specimens of I. ciosi, I. epshteini or I. bieleckii sp. n. or another new species of the genus Italobdella. To complete the picture of the Caspian Piscicolidae, the presence of two species on the other side (eastern) of the Adriatic Sea is also important. The first is Caspiobdella hadzi (Sket 1968) which was described as Piscicola hadzi Sket, 1968 after interpreting the reproductive system of this species, moved it to the genus Caspiobdella. The same happened with Cystobranchus pawlowskii Sket, 1968, which was transferred to the genus Piscicola (Sket 1968).
Concluding, in Eurasia we distinguish between two neolimic groups of the Piscicolidae family: the eastern one, which is represented by Limnotrachelobdella and genera related to Baikal leeches, and the western one, which contains the leeches classified in the Caspian type.
2. Relatively big, body length up to 21.1 mm, on anterior sucker large white "spectacles", coloration of dorsal side of trachelosome and urosome distinct -a characteristic pattern formed by brown, black, yellow and white melanophores whose arrangement results in white transverse segmental spots; somite consisting of 4 annuli (with further divisions into 12

Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/.