Abstract
Cryptomeria japonica var. sinensis (Cupressaceae) is an important plantation species that is native to China. Based on the complete chloroplast genome sequence, we developed 11 novel polymorphic chloroplast microsatellite markers and assessed their genetic characterization among three populations. A total of 30 haplotypes were detected in the three populations, and the number of haplotypes per population ranged from 7 to 15. The number of private haplotypes detected in the three populations ranged from 7 to 12. The effective number of haplotypes ranged from 2.90 to 10.37 with an average of 5.84 and the haplotypic richness (Rh) ranged from 6.00 to 12.54. Genetic diversity (He) ranged from 0.69 to 0.95 with an average of 0.81. The number of alleles per locus and population ranged from 1 to 4, and the effective number of alleles ranged from 1.00 to 3.21. In addition, these novel markers were tested by cross-species amplification in four Cupressaceae species. Except for the Cf_cp_12 locus, all polymorphic loci can successfully amplify in four Cupressaceae species: Glyptostrobus pensilis, Taiwania cryptomerioides, C. japonica, and Cunninghamin lanceolata. The chloroplast microsatellite markers reported here will facilitate the characterization of the population genetic and phylogeographic analyses of C. japonica var. sinensis effectively.
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Data availability
Sequence information for the developed primers has been deposited to NCBI; accession numbers are provided in Table 1.
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Acknowledgments
This project is supported by Key projects of international scientific and technological innovation cooperation between governments of China (2016YFE0127200).
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YW designed the study; YT provided the samples of C. japonica var. japonica; MW, MY, XL, XW, and MC performed the experiments; SU performed the sequence analysis and primer design; MW, MY and, XL analyzed the data and wrote the draft of the manuscript; YW, SU, and YT revised the manuscript. The decision to submit the manuscript for publication was made by all the authors. All authors read and approved the final manuscript.
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Wang, M., Yuan, M., Li, X. et al. Development and characterization of novel chloroplast microsatellite markers for Cryptomeria japonica var. sinensis (Cupressaceae) and their cross-species amplification. Biologia 77, 361–367 (2022). https://doi.org/10.1007/s11756-021-00946-z
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DOI: https://doi.org/10.1007/s11756-021-00946-z