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A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine in real water samples and its bioimaging in vivo and in vitro

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Abstract

Hydrazine is extremely toxic and causes severe harm to human body. Herein, a novel fluorescent probe 4-oxo-2-styryl-4H-chromen-3-yl thiophene-2-carboxylate (FHT) was synthesized for detecting hydrazine by using natural cinnamaldehyde as starting material. This probe exhibited significantly enhanced fluorescence response towards hydrazine over various common metal ions, anions, and amine compounds. The detection limit of probe FHT for hydrazine was as low as 0.14 µmol·L−1, significantly lower than that of the threshold value of 0.312 µmol·L−1, imposed by the Environmental Protection Agency. Moreover, the proposed probe was able to detect hydrazine within wide pH (5–10) and linear detection ranges (0–110 µmol·L−1). This probe was employed for determining trace hydrazine in different environmental water samples. The probe FHT-loaded filter paper strips were able to conveniently detect hydrazine of low concentration through distinct naked-eye and fluorescent color changes. Importantly, the probe FHT with low cytotoxicity was successfully applied to visualize hydrazine in living Hela cells and zebrafish.

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Acknowledgments

We thank the financial assistance from the National Natural Science Foundation of China (Grant Nos. 32071707 and 32101466) and the Natural Science Foundation of Jiangsu Province (No. BK20210624).

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Authors and Affiliations

  1. Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China

    Ahui Qin, Yan Zhang, Shuai Gong, Mingxin Li, Yu Gao, Xu Xu, Zhonglong Wang & Shifa Wang

  2. Department of Natural Sciences, University of Michigan-Flint, Flint, MI, 48502, USA

    Jie Song

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  1. Ahui Qin
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  2. Yan Zhang
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  3. Shuai Gong
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  4. Mingxin Li
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  5. Yu Gao
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  6. Xu Xu
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  7. Jie Song
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  8. Zhonglong Wang
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  9. Shifa Wang
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Correspondence to Zhonglong Wang or Shifa Wang.

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11705_2022_2171_MOESM1_ESM.pdf

A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine in real water samples and its bioimaging in vivo and vitro

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Qin, A., Zhang, Y., Gong, S. et al. A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine in real water samples and its bioimaging in vivo and in vitro. Front. Chem. Sci. Eng. 17, 24–33 (2023). https://doi.org/10.1007/s11705-022-2171-1

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  • DOI: https://doi.org/10.1007/s11705-022-2171-1

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