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RGS16 regulated by let-7c-5p promotes glioma progression by activating PI3K-AKT pathway

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Abstract

Gliomas are the most common central nervous system tumours; they are highly aggressive and have a poor prognosis. RGS16 belongs to the regulator of G-protein signalling (RGS) protein family, which plays an important role in promoting various cancers, such as breast cancer, pancreatic cancer, and colorectal cancer. Moreover, previous studies confirmed that let-7c-5p, a well-known microRNA, can act as a tumour suppressor to regulate the progression of various tumours by inhibiting the expression of its target genes. However, whether RGS16 can promote the progression of glioma and whether it is regulated by miR let-7c-5p are still unknown. Here, we confirmed that RGS16 is upregulated in glioma tissues and that high expression of RGS16 is associated with poor survival. Ectopic deletion of RGS16 significantly suppressed glioma cell proliferation and migration both in vitro and in vivo. Moreover, RGS16 was validated as a direct target gene of miR let-7c-5p. The overexpression of miR let-7c-5p obviously downregulated the expression of RGS16, and knocking down miR let-7c-5p had the opposite effect. Thus, we suggest that the suppression of RGS16 by miR let-7c-5p can promote glioma progression and may serve as a potential prognostic biomarker and therapeutic target in glioma.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (Nos. 81874083, 82072776, 82072775, 81702468, 81802966, 81902540, 81874082, and 81472353), Natural Science Foundation of Shandong Province of China (Nos. ZR2019BH057, ZR2020QH174, and ZR2021LSW025), the Jinan Science and Technology Bureau of Shandong Province (No. 2021GXRC029), Key Clinical Research Project of Clinical Research Center of Shandong University (No. 2020SDUCRCA011) and Taishan Scholars of Shandong Province of China (No. tspd20210322), and Scientific Research Foundation of Qilu Hospital (Qingdao) (No. QDKY2019QN03).

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Authors and Affiliations

  1. Department of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan, 250012, China

    Chaochao Wang, Hao Xue, Rongrong Zhao, Xiao Gao, Yanhua Qi, Huizhi Wang, Jianye Xu, Lin Deng & Gang Li

  2. Shandong Key Laboratory of Brain Function Remodeling, Jinan, 250012, China

    Chaochao Wang, Hao Xue, Rongrong Zhao, Zhongzheng Sun, Xiao Gao, Yanhua Qi, Huizhi Wang, Jianye Xu, Lin Deng & Gang Li

  3. The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250033, China

    Zhongzheng Sun

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  1. Chaochao Wang
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  2. Hao Xue
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  9. Lin Deng
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  10. Gang Li
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Correspondence to Lin Deng or Gang Li.

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Chaochao Wang, Hao Xue, Rongrong Zhao, Zhongzheng Sun, Xiao Gao, Yanhua Qi, Huizhi Wang, Jianye Xu, Lin Deng, and Gang Li declare that they have no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.

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Wang, C., Xue, H., Zhao, R. et al. RGS16 regulated by let-7c-5p promotes glioma progression by activating PI3K-AKT pathway. Front. Med. 17, 143–155 (2023). https://doi.org/10.1007/s11684-022-0929-y

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  • DOI: https://doi.org/10.1007/s11684-022-0929-y

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