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Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium

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Abstract

The enhancer of zeste 2 polycomb repressive complex 2 subunit (Ezh2) is a histone-lysine Nmethyltransferase enzyme that participates in DNA methylation. Ezh2 has also been reported to play crucial roles in stem cell proliferation and differentiation. However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated. Here, we examined the spatiotemporal expression of Ezh2 in the cochlea during embryonic and postnatal development. Ezh2 expression began to be observed in the whole otocyst nuclei at embryonic day 9.5 (E9.5). At E12.5, Ezh2 was expressed in the nuclei of the cochlear prosensory epithelium. At E13.5 and E15.5, Ezh2 was expressed from the apical to the basal turns in the nuclei of the differentiating cochlear epithelium. At postnatal day (P) 0 and 7, the Ezh2 expression was located in the nuclei of the cochlear epithelium in all three turns and could be clearly seen in outer and inner hair cells, supporting cells, the stria vascularis, and spiral ganglion cells. Ezh2 continued to be expressed in the cochlear epithelium of adult mice. Our results provide the basic Ezh2 expression pattern and might be useful for further investigating the detailed role of Ezh2 during cochlear development.

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Authors and Affiliations

  1. Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200031, China

    Yan Chen, Wenyan Li, Wen Li & Huawei Li

  2. Central Laboratory, Affiliated Eye and ENT Hospital, Fudan University, Shanghai, 200031, China

    Yan Chen & Wen Li

  3. Key Laboratory of Hearing Medicine of the National Health and Family Planning Commission, Shanghai, 200031, China

    Yan Chen, Wenyan Li, Wen Li & Huawei Li

  4. MOE Key Laboratory of Developmental Genes and Human Disease, State Key Laboratory of Bioelectronics, Institute of Life Sciences, Southeast University, Nanjing, 210096, China

    Renjie Chai

  5. Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China

    Renjie Chai

  6. Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China

    Huawei Li

Authors
  1. Yan Chen
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  2. Wenyan Li
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  3. Wen Li
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  4. Renjie Chai
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  5. Huawei Li
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Correspondence to Renjie Chai or Huawei Li.

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These authors contributed equally to this work.

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Chen, Y., Li, W., Li, W. et al. Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium. Front. Med. 10, 330–335 (2016). https://doi.org/10.1007/s11684-016-0459-6

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  • DOI: https://doi.org/10.1007/s11684-016-0459-6

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