Fronto-striatal alterations correlate with apathy severity in behavioral variant frontotemporal dementia

Structural and functional changes in cortical and subcortical regions have been reported in behavioral variant frontotemporal dementia (bvFTD), however, a multimodal approach may provide deeper insights into the neural correlates of neuropsychiatric symptoms. In this multicenter study, we measured cortical thickness (CTh) and subcortical volumes to identify structural abnormalities in 37 bvFTD patients, and 37 age- and sex-matched healthy controls. For seed regions with significant structural changes, whole-brain functional connectivity (FC) was examined in a sub-cohort of N = 22 bvFTD and N = 22 matched control subjects to detect complementary alterations in brain network organization. To explore the functional significance of the observed structural and functional deviations, correlations with clinical and neuropsychological outcomes were tested where available. Significantly decreased CTh was observed in the bvFTD group in caudal middle frontal gyrus, left pars opercularis, bilateral superior frontal and bilateral middle temporal gyrus along with subcortical volume reductions in bilateral basal ganglia, thalamus, hippocampus, and amygdala. Resting-state functional magnetic resonance imaging showed decreased FC in bvFTD between: dorsal striatum and left caudal middle frontal gyrus; putamen and fronto-parietal regions; pallidum and cerebellum. Conversely, bvFTD showed increased FC between: left middle temporal gyrus and paracingulate gyrus; caudate nucleus and insula; amygdala and parahippocampal gyrus. Additionally, cortical thickness in caudal, lateral and superior frontal regions as well as caudate nucleus volume correlated negatively with apathy severity scores of the Neuropsychiatry Inventory Questionnaire. In conclusion, multimodal structural and functional imaging indicates that fronto-striatal regions have a considerable influence on the severity of apathy in bvFTD. Supplementary Information The online version contains supplementary material available at 10.1007/s11682-023-00812-3.


Introduction
Frontotemporal dementia (FTD) is the second most common form of dementia in early age (< 65) (Onyike & Diehl-Schmid, 2013), clinically characterized by behavioral impairment, executive dysfunction and language deterioration.Phenotypes with predominant behavioral impairments and primary executive dysfunction are classified as behavioral variant FTD (bvFTD), while patients with language impairment are classified as primary progressive aphasia (Neary et al., 1998).50-70% of the FTD patients are diagnosed as bvFTD (Johnson et al., 2005).
Studies have assessed the neuroanatomical underpinnings of apathy and disinhibition in bvFTD (Lansdall et al., 2017;Kumfor et al., 2018;Wei et al., 2020;Sheelakumari et al., 2020).Widespread volumetric changes were reported in frontal, temporal, and limbic areas along with cortical thickness (CTh) reductions in frontal, temporal and insular regions (Möller et al., 2016).Functional changes were studied using resting-state functional magnetic resonance imaging (rsfMRI), providing measures of spontaneous brain activity and functional connectivity (FC) (Azeez & Biswal, 2017).Decreased FC in bvFTD was reported within/between lateral prefrontal, basal ganglia, insular, hippocampal and amygdalar regions as well as salience and default mode networks (Ferreira et al., 2022;Kamalian et al., 2022).Conversely, increased FC was reported within the default mode network (Filippi et al., 2013;Whitwell et al., 2011).A relationship between altered mind-wandering capacity and structural / functional integrity of default and frontoparietal networks was reported in bvFTD using a multimodal approach (O'Callaghan et al., 2019).Another multimodal study reported that cortical thinning in temporal and orbitofrontal regions could predict clinical diagnosis of bvFTD (Canu et al., 2017).However, no study has as yet assessed structural alterations to determine seeds regions for analysis of FC and their relationship with neuropsychiatric symptoms within the same bvFTD cohort.Therefore, this study implemented a multimodal approach that first assessed surface-based CTh and subcortical volume changes and, second, examined "seed-to-whole brain" FC from brain areas showing structural alterations in bvFTD patients compared to matched HCs.We hypothesized structural changes in dorsolateral, middle frontal and temporal regions and associated reduced FC linked with behavioral symptoms in bvFTD.

Methods
Seventy-nine FTD patients were recruited in different clinics affiliated to the multicenter DESCRIBE (DZNE Clinical Register Study of Neurodegenerative Disorders) study at the German Center of Neurodegenerative Diseases (DZNE e.V.).Thirty-seven were diagnosed as bvFTD (Rascovsky et al., 2011) (age (mean ± SD) = 63.81 ± 11.32; sex (M:F) = 21:16) and included in the study.Five patients showed C9orf72 mutations, 1 had VCP and 1 FUS mutations.
Three individuals had no DNA samples and the remaining 27 patients did not exhibit any mutations.Diagnostic criteria for bvFTD were presence of any three of clinically characterizing features like apathy/inertia, loss of sympathy, perseverative/compulsive behaviors, hyperorality, disinhibition and executive dysfunction (Rascovsky et al., 2011).The control group consisted of 37 age-(64.78± 7.63) and sex-(M: F = 22:15) matched healthy controls (HC).
Statements on Ethics and the Declaration of Helsinki are given in the Supplement, as well as inclusion and exclusion criteria and clinical, behavioral, neuropsychological measures.MRI data acquisition, analysis and statistical analysis is also mentioned in the Supplement.
For the demographic data of sub cohort of 22 bvFTD with complementary resting fMRI data and 22 matched controls see the supplementary Table 1.

Cortical thickness
A significant bilateral cortical thinning was observed in bvFTD patient group in the left hemisphere, affecting caudal middle frontal gyrus (CMFG), middle temporal gyrus (MTG), pars opercularis and superior frontal gyrus (SFG).In the right hemisphere, only MTG and SFG showed significant cortical thinning (Fig. 1 part 1.1).

Subcortical volume
A general linear model implementing age, sex, education, total intracranial volume and scanner site as nuisance variables showed significant bilateral volume losses in thalamus, caudate nucleus, putamen, pallidum, amygdala, hippocampus and nucleus accumbens in bvFTD (Supplementary Table 2).

Cortical seeds-to-whole-brain FC
Among all cortically thinned regions only left CMFG and left MTG showed significant FC changes in bvFTD.Furthermore, left caudal middle frontal gyrus showed decreased FC with dorsal striatum, anterior thalamus and cerebellar regions (Fig. 1 part 1.2a).Conversely, left MTG showed increased FC with paracingulate gyrus (Fig. 1 part 1.2b).

Subcortical seeds-to-whole-brain FC
For subcortical regions, decreased and increased FC was observed in bvFTD: Putamen showed decreased FC with cingulate, medial, lateral frontal and parietal cortex (Fig. 1 part 1.3a) and pallidum with medial and lateral cerebellar lobes (Fig. 1 part 1.3b).Conversely, caudate nucleus showed increased FC with right insula and inferior frontal gyrus (Fig. 1 part 1.3c).Amygdala and hippocampus showed increased FC with right parahippocampal gyrus, temporal pole and central opercular cortex (Fig. 1 parts 1.3d and 1.3e, respectively).No FC changes occurred in networks related to thalamus and nucleus accumbens seeds.

Discussion
This multimodal MRI study investigated structural and functional changes and their association with neuropsychiatric symptoms, notably apathy, disinhibition and executive  18) 0.031* *Shows significant differences at p < 0.05, ** shows highly significant, § represent the variables showing the maximum subjects in bracket with available scores for the corresponding test in each group (bvFTD patients as well as healthy controls).MMSE: mini mental screening examination; CDR-SOB: clinical dementia rating scale-sum of boxes; NPI: neuropsychiatric inventory; GDS: Geriatric depression scale; HSCT: Hayling sentence completion test; DS: Digit span; nc: number of correct sequences.M: male; F: female; nc: number of correct responses; sec.: seconds Table 1 Represent the demographic, clinical and behavioral measures of 37 bvFTD and 37 age and-gender matched healthy controls in left CMFG, left pars opercularis, and right SFG to correlate with apathy severity.Middle frontal areas are involved in motivated behaviors (Kouneiher et al., 2009) whereas lateral frontal areas are more involved in cognitive control (Bahlmann et al., 2015).Thus, we speculate that the structural reductions cause amotivated behavior and reduced cognitive control (shown by higher scores for HSCT error of category A), possibly leading to apathetic behavior in bvFTD.
Affection of the basal ganglia also seems to play a distinctive role for apathy (Jenkins et al., 2022).Basal ganglia atrophy, especially of caudate nucleus, putamen and pallidum, has been reported in bvFTD (Bertoux et al., 2015;Macfarlane et al., 2015) and was reported to be relevant for apathy in bvFTD (Jenkins et al., 2022).Our observations of caudate atrophy in relation with increased apathy severity gives further support to the striatal role for apathy in bvFTD (Bertoux et al., 2015;Kumfor et al., 2018).
Beyond the independent role of the frontal cortex and the basal ganglia for apathy outlined above, fronto-striatal circuit impairment has been linked to apathy in elderly individuals (Hamada et al., 2021).The novelty of the current dysfunction in patients with bvFTD.Significant cortical thinning was found in frontal and temporal regions in bvFTD, along with subcortical volumetric reductions in all seven tested regions.Assessment of FC in seed regions obtained from structural analyses helped identifying functional changes associated with underlying structural loss: Decreased FC was found between left CMFG and anterior caudate nucleus and increased FC between left MTG and paracingulate gyrus.Similarly, subcortical seed regions also showed both decreased and increased FC in bvFTD.Putamen and pallidum showed decreased FC with fronto-parietal and cerebellar areas, respectively, while caudate nucleus, amygdala and hippocampus demonstrated increased FC with insula, inferior frontal and parahippocampus gyrus.Additionally, correlation analyses between clinical, behavioral and structural measures suggest that cortical thinning in frontal regions and volume loss in caudate nucleus relate with apathy severity in bvFTD.
Previous studies reported lateral and dorsal frontal areas to be predominantly linked with apathy in bvFTD (Moretti & Signori, 2016;Ducharme et al., 2018;Sheelakumari et al., 2020;Jenkins et al., 2022).We found cortical thinning Fig. 1 Display of structural changes in 37 bvFTD as compared to 37 healthy controls: 1.1) Decreased cortical thickness in 37 bvFTD (blue color) as well as Changes in FC when considered regions exhibiting cortical thinning and sub cortical volume loss as seeds in 22 bvFTD compared to 22 HC; 1.2a) Decreased FC with respect to left caudal middle frontal gyrus and; 1.2b) Increased FC with respect to left middle temporal gyrus; 1.3a) decreased FC with respect to putamen; 1.3b) decreased FC with respect to pallidum; and 1.3c) increased FC with respect to caudate nucleus; 1.3d) increased FC with respect to amygdala; and 1.3e) increased FC with respect to hippocampus.Here, decrease in FC is presented with blue color while increase with redyellow.FC = functional connectivity; L = left; R = right study is the demonstration of fronto-striatal circuit impairment, indexed as altered FC, in bvFTD.Notably, decreased FC between left CMFG and dorsal striatum was a principal finding, suggesting perturbation of fronto-striatal networks in bvFTD.Moreover, NPI-Q sub-scores for apathy severity were inversely correlated with cortical thickness in frontal and volume loss in striatal regions, strengthening the pathophysiological relevance of our multimodal approach in bvFTD.
In conclusion, the presented data indicate that structural alterations in frontal and striatal regions lead to disintegration of fronto-striatal networks in bvFTD and contribute to apathy severity, a core clinical symptom of the disease.Meanwhile, we acknowledge the small sample size and some missing behavioral measures.Future multimodal studies with bigger sample sizes and complete data sets warrant further validation of our observations.