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Genome-wide identification and analysis of the DREB genes and their expression profiles under abiotic stresses in Chinese jujube (Ziziphus jujuba Mill.)

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Abstract

CBF/DREB proteins play a critical role in abiotic stress-mediated gene expression and represent attractive regulons for plant breeding programs. However, no study has been conducted for CBF/DREB protein-related genes in jujube (Ziziphus jujuba Mill.). In this study, twenty-five ZjDREB genes were identified and annotated from the jujube (Z. jujuba ‘Dongzao’) genome. Detailed analysis, including gene classification, annotation, phylogenetic evaluation, conserved motif determination and expression profiling were performed on all genes. Phylogenetic analysis showed that ZjDREB proteins were divided into five subgroups (A1–A5), but lacking a subgroup A6 corresponding to AtDREBs. The ZjDREB genes were distributed in nine of twelve chromosomes in the genome. Additionally, the expression patterns of the DREB genes under different abiotic stresses were investigated using qRT-PCR. Nineteen ZjDREB genes were down-regulated under low temperature, in contrast six ZjDREB genes (01, 03, 05, 11, 23 and 24) were up-regulated. Under drought, salinity and high temperature conditions, expression of ZjDREB03, 09, 10, 14, 15, 17 and 20 genes were induced and showed similar expression patterns, suggesting that various stress conditions share common elements in the signaling pathway. The results suggest that the family of DREB genes play an important role in abiotic stresses in jujube, and provide a foundation for further functional studies of this important class of transcriptional regulators.

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Authors and Affiliations

  1. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China

    Heying Zhou, Jiping Jia, Zhendong Zhang, Shuang Song, Yingyue Li & Xiaoming Pang

  2. National Foundation for Improved Cultivar of Chinese Jujube, Cangzhou, 061000, Hebei, China

    Decang Kong

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  1. Heying Zhou
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  2. Jiping Jia
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  3. Decang Kong
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  4. Zhendong Zhang
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  5. Shuang Song
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  6. Yingyue Li
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  7. Xiaoming Pang
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Correspondence to Xiaoming Pang.

Additional information

Project funding: This work was funded by the National Natural Science Foundation of China (31372019), Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, P. R. China (kf2017015) and the Beijing Municipal Education Commission (CEFF-PXM2017_014207_000043).

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Corresponding editor: Hu Yanbo.

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Zhou, H., Jia, J., Kong, D. et al. Genome-wide identification and analysis of the DREB genes and their expression profiles under abiotic stresses in Chinese jujube (Ziziphus jujuba Mill.). J. For. Res. 30, 1277–1287 (2019). https://doi.org/10.1007/s11676-018-0718-2

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  • DOI: https://doi.org/10.1007/s11676-018-0718-2

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