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In Vitro Study on Cytocompatibility of Mg Wire/Poly(Lactic Acid) Composite Rods

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Abstract

In order to ensure the safety of materials used in the field of orthopedic internal fixation, cytocompatibility was evaluated by culturing MC3T3-E1 cells with the extracts of Mg-2Zn rods, poly(lactic acid) (PLA) rods, hot pressed and hot drawn Mg wire/PLA composite rods. The adhesion, spread, and growth states of cells on each group of sample were analyzed according to SEM images. The fluorescent micromorphology was analyzed, and the relative growth ratio was calculated to quantitatively evaluate the influence of these extracts on the cell viability. Results displayed that moderate and severe cytotoxicity appeared after cells incubated with extracts of Mg-2Zn rod for 3 and 5 days, but it returned to normal phenomenon after the extracts diluted by 10 times. Extracts of other three groups of sample displayed good cytocompatibility. The growth and proliferation of cells were closely related to the pH value and concentration of Mg2+. The osmolality changes of extracts had little influence on cytocompatibility of samples at the early stage. Therefore, we can predict the effect of degradation products of Mg wire/PLA composites on their cytocompatibility during the degradation process, on the basis of pH value, concentration of Mg2+, as well as osmolality changes. The good cytocompatibility of Mg wire/PLA composites provided experimental valid and valuable guideline for further material design and scheme selection.

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Acknowledgments

This work was jointly supported by National Natural Science Foundation of China (No. 51771054, No. 51971062), Science and Technology Project of Jiangsu Province (No. BE2019679), Introduction of Talent Research Fund in Nanjing Institute of Technology (YKJ202008, YKJ201704), Natural Science Foundation of Jiangsu Province (BK20181020) and Outstanding Scientific and Technological Innovation Team in Colleges and Universities of Jiangsu Province.

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Authors and Affiliations

  1. School of Materials Science and Engineering, Nanjing Institute of Technology, Nanjing, 211167, Jiangsu, China

    Hong Cai, Xuan Li & Xiaobo Zhang

  2. Jiangsu Key Laboratory of Advanced Structural Materials and Application Technology, Nanjing Institute of Technology, Nanjing, 211167, Jiangsu, China

    Hong Cai, Xuan Li & Xiaobo Zhang

  3. School of Materials Science and Engineering, Southeast University, Jiangning, Nanjing, 211189, Jiangsu, China

    Feng Xue, Chenglin Chu, Chao Guo & Jing Bai

  4. Jiangsu Key Laboratory for Advanced Metallic Materials, Jiangning, Nanjing, 211189, Jiangsu, China

    Feng Xue, Chenglin Chu, Chao Guo & Jing Bai

  5. Institute of Biomedical Devices (Suzhou), Southeast University, Suzhou, 215163, Jiangsu, China

    Jing Bai

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  1. Hong Cai
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Contributions

HC was involved in methodology, investigation, data acquisition and analysis, writing original draft. XL contributed to funding support, writing review, and edit. FX was involved in funding support, supervision. CC contributed to funding support. CG was involved in validation. JB contributed to project administration. XZ was involved in edit.

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Correspondence to Hong Cai or Xuan Li.

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Cai, H., Li, X., Xue, F. et al. In Vitro Study on Cytocompatibility of Mg Wire/Poly(Lactic Acid) Composite Rods. J. of Materi Eng and Perform 30, 7214–7222 (2021). https://doi.org/10.1007/s11665-021-05883-1

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