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Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF- κ B and PI3K/AKT Signaling In Vitro and In Vivo

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Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To explore the influences of andrographolide (Andro) on bladder cancer cell lines and a tumor xenograft mouse model bearing 5637 cells.

Methods

For in vitro experiments, T24 cells were stimulated with Andro (0–40 µmol/L) and 5637 cells were stimulated with Andro (0 to 80 µmol/L). Cell growth, migration, and infiltration were assessed using cell counting kit-8, colony formation, wound healing, and transwell assays. Apoptosis rate was examined using flow cytometry. In in vivo study, the antitumor effect of Andro (10 mg/kg) was evaluated by 5637 tumor-bearing mice, and levels of nuclear factor κ B (NF- κ B) and phosphoinositide 3-kinase/AKT related-proteins were determined by immunoblotting.

Results

Andro suppressed growth, migration, and infiltraion of bladder cancer cells (P⩽0.05 or P⩽0.01). Additionally, Andro induced intrinsic mitochondria-dependent apoptosis in bladder cancer cell lines. Furthermore, Andro inhibited bladder cancer growth in mice (P⩽0.01). The expression of p65, p-AKT were suppressed by Andro treatment in vitro and in vivo (P⩽0.05 or P⩽0.01).

Conclusions

Andrographolide inhibits proliferation and promotes apoptosis in bladder cancer cells by interfering with NF- κ B and PI3K/AKT signaling in vitro and in vivo.

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Authors and Affiliations

  1. Department of Urology, the First Hospital of Jilin University, Changchun, 130021, China

    Lei Xuan, Jing-hai Hu, Ran Bi, Si-qi Liu &  Chun-xi Wang

Authors
  1. Lei Xuan
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  2. Jing-hai Hu
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  3. Ran Bi
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  4. Si-qi Liu
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  5. Chun-xi Wang
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Contributions

Wang CX designed the experiments and reviewed the final manuscript. Xuan L performed the experiments and wrote the first draft of the manuscript. Hu JH and Bi R contributed to analyzed the data. Liu SQ was responsible for some animal studies. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Chun-xi Wang.

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The authors declare no conflict of interest.

Additional information

Supported by Science and Technology Department of Jilin Province (No. 20190905001SF)

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Xuan, L., Hu, Jh., Bi, R. et al. Andrographolide Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer Cells by Interfering with NF- κ B and PI3K/AKT Signaling In Vitro and In Vivo. Chin. J. Integr. Med. 28, 349–356 (2022). https://doi.org/10.1007/s11655-022-3464-4

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  • DOI: https://doi.org/10.1007/s11655-022-3464-4

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