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miR-106b enhances human mesenchymal stem cell differentiation to spermatogonial stem cells under germ cell profile genes involved in TGF-b signaling pathways

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Abstract

Mesenchymal stem cells can be differentiated into tissue-specific cells. MicroRNAs (miRNAs) regulate the translation of mRNAs involved in the growth and development of a variety of cells, including primordial germ cells (PGCs). This study evaluated male germ cell differentiation from human MSCs by miR-106b. The MSCs were obtained from human adipose tissue. The differentiation of MSCs into PGCs was accomplished by transfection of a lentiviral vector expressing miR-106b. MSCs were treated with bone morphogenic factor 4 as a control and also as a putative inducer of PGC differentiation. PGC was differentiated into spermatogonial-like cells by retinoic acid. Moreover, Dazl, Plzf, Stra8, Gfra, and Thy1 gene expressions were investigated using real-time PCR. Our results showed that Dazl, Plzf, and Stra8 genes that were treated with BMP4 and miR-106b did not show any significant difference, meaning that miR-106b, like BMP4, is able to differentiate PGC cells from MSCs. In spermatogonial-like cells, Thy1 was significantly unregulated in both the miR-106b and BMP4 groups. Our findings showed that miR-106b regulates the differentiation of MSCs into PGCs. miR-106b influences on the expression of Dazl, Plzf, and Stra8 genes in PGC and Gfra, Stra8, and Thy1 genes.

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Acknowledgements

We are thankful to the Histogenotech Company, Tehran, Iran, for providing necessary laboratory facilities.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Sadaf Mahboudi, Kazem Parivar & Shiva Irani

  2. Basic Medical Sciences Research center, Histogenotech Company, Tehran, 1441633665, Iran

    Zohreh Mazaheri

Authors
  1. Sadaf Mahboudi
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  2. Kazem Parivar
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  3. Zohreh Mazaheri
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  4. Shiva Irani
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Contributions

Sadaf Mahboudi carried out the experiments and wrote the manuscript draft; Kazem Parivar contributed to the research design and data validation, and supervised the project; Zohreh Mazaheri contributed to the research design, interpretation of data, and revision of manuscript; and Shiva Irani contributed to statistical analysis and helped to draft the manuscript. All authors read and approved the final manuscript.

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Correspondence to Zohreh Mazaheri.

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Mahboudi, S., Parivar, K., Mazaheri, Z. et al. miR-106b enhances human mesenchymal stem cell differentiation to spermatogonial stem cells under germ cell profile genes involved in TGF-b signaling pathways. In Vitro Cell.Dev.Biol.-Animal 58, 539–548 (2022). https://doi.org/10.1007/s11626-022-00688-5

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  • DOI: https://doi.org/10.1007/s11626-022-00688-5

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