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Integrated analysis of gene expression and methylation profiles of novel pancreatic cancer cell lines with highly metastatic activity

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Abstract

Pancreatic cancer is one of the most lethal human malignancies, partly because of its propensity for metastasis. However, highly metastatic human pancreatic cancer cell lines suitable for studies of metastasis are currently lacking. Here we established two highly metastatic human pancreatic cancer cell lines, MIA PaCa-2 In8 and Panc-1 In8, by Matrigel induction assay. The cell lines were further characterized both in vitro and in vivo. MIA PaCa-2 In8 and Panc-1 In8 cells demonstrated increased migration and invasion compared with their respective parental cells. Following injection into nude mice, MIA PaCa-2 In8 and Panc-1 In8 cells resulted in more pulmonary metastases compared with the parental cells. Furthermore, analyses of mRNA, long non-coding RNA, micro RNA, and methylation profiling revealed that these factors were aberrantly regulated in the highly metastatic cells, indicating that they probably affected metastasis. We thus established and characterized two highly metastatic human pancreatic cell lines that could be used as valuable tools for future investigations into the pathogenesis, metastasis, and potential treatment of human pancreatic cancer.

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Acknowledgements

This work was supported by the CAMS Innovation Fund for Medical Sciences (CIFMS) (2016-12M-3-005 and 2016-I2M-1-001), PUMC Youth Fund and the Fundamental Research Funds for the Central Universities (2017320027), the National Natural Science Foundation of China (81772639), and the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences (2018PT32014).

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  1. Contributed equally to this work

Authors and Affiliations

  1. Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China

    Gang Yang, Huanyu Wang, Mengyu Feng, Lei You, Taiping Zhang, Lin Cong & Yupei Zhao

  2. Department of Nuclear Medicine, Peking Union Medical Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China

    Lianfang Zheng

  3. Clinical Immunology Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China

    Taiping Zhang

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  1. Gang Yang
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Correspondence to Lin Cong or Yupei Zhao.

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Yang, G., Wang, H., Feng, M. et al. Integrated analysis of gene expression and methylation profiles of novel pancreatic cancer cell lines with highly metastatic activity. Sci. China Life Sci. 62, 791–806 (2019). https://doi.org/10.1007/s11427-018-9495-2

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  • DOI: https://doi.org/10.1007/s11427-018-9495-2

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