Skip to main content
SpringerLink
Log in

A highly sensitive DNA-AIEgen-based “turn-on” fluorescence chemosensor for amplification analysis of Hg2+ ions in real samples and living cells

  • Articles
  • Published:
Science China Chemistry Aims and scope Submit manuscript

Abstract

Functional nucleic acids (FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled (fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids (AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease (DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg2+ are achieved. This robust assay allows for sensitive and selective detection of Hg2+ in real water samples and mapping of intracellular Hg2+, without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Valeur B, Leray I. Coordin Chem Rev, 2000, 205: 3–40

    Article  CAS  Google Scholar 

  2. Kim HN, Ren WX, Kim JS, Yoon J. Chem Soc Rev, 2012, 41: 3210–3244

    Article  CAS  Google Scholar 

  3. Tian Y, Wang Y, Xu Y, Liu Y, Li D, Fan C. Sci China Chem, 2015, 58: 514–518

    Article  CAS  Google Scholar 

  4. Zhao Y, Zhong Z. J Am Chem Soc, 2006, 128: 9988–9989

    Article  CAS  Google Scholar 

  5. Cheng X, Li S, Jia H, Zhong A, Zhong C, Feng J, Qin J, Li Z. Chem Eur J, 2012, 18: 1691–1699

    Article  CAS  Google Scholar 

  6. Huang CC, Chang HT. Anal Chem, 2006, 78: 8332–8338

    Article  CAS  Google Scholar 

  7. Wang H, Wang Y, Jin J, Yang R. Anal Chem, 2008, 80: 9021–9028

    Article  CAS  Google Scholar 

  8. Cheng X, Li Q, Qin J, Li Z. ACS Appl Mater Interfaces, 2010, 2: 1066–1072

    Article  CAS  Google Scholar 

  9. Xu M, Gao Z, Wei Q, Chen G, Tang D. Biosens Bioelectron, 2016, 79: 411–415

    Article  CAS  Google Scholar 

  10. Neupane LN, Oh ET, Park HJ, Lee KH. Anal Chem, 2016, 88: 3333–3340

    Article  CAS  Google Scholar 

  11. Ou S, Lin Z, Duan C, Zhang H, Bai Z. Chem Commun, 2006, 42: 4392–4394

    Article  Google Scholar 

  12. Li D, Wieckowska A, Willner I. Angew Chem Int Ed, 2008, 47: 3927–3931

    Article  CAS  Google Scholar 

  13. Hatai J, Pal S, Jose GP, Bandyopadhyay S. Inorg Chem, 2012, 51: 10129–10135

    Article  CAS  Google Scholar 

  14. Srivastava P, Razi SS, Ali R, Gupta RC, Yadav SS, Narayan G, Misra A. Anal Chem, 2014, 86: 8693–8699

    Article  CAS  Google Scholar 

  15. Shellaiah M, Rajan YC, Balu P, Murugan A. New J Chem, 2015, 39: 2523–2531

    Article  CAS  Google Scholar 

  16. Najafi E, Aboufazeli F, Lotfi Zadeh Zhad HR, Sadeghi O, Amani V. Food Chem, 2013, 141: 4040–4045

    Article  CAS  Google Scholar 

  17. Zhu M, Wang Y, Deng Y, Yao L, B. Adeloju S, Pan D, Xue F, Wu Y, Zheng L, Chen W. Biosens Bioelectron, 2014, 61: 14–20

    Article  CAS  Google Scholar 

  18. Condomitti U, Zuin A, Silveira AT, Toma SH, Araki K, Toma HE. Electroanalysis, 2011, 23: 2569–2573

    Article  CAS  Google Scholar 

  19. Hezard T, Laffont L, Gros P, Behra P, Evrard D. J Electroanal Chem, 2013, 697: 28–31

    Article  CAS  Google Scholar 

  20. Liu J, Cao Z, Lu Y. Chem Rev, 2009, 109: 1948–1998

    Article  CAS  Google Scholar 

  21. Xu L, Xiang J, Peng R, Liu Z. Sci Bull, 2016, 61: 514–523

    Article  CAS  Google Scholar 

  22. Gao W, Wang X, Fan H, Song Z, Lai X, Chen Z, Tan W. Sci Bull, 2015, 60: 1101–1107

    Article  CAS  Google Scholar 

  23. Chao J, Fan C. Sci China Chem, 2015, 58: 834–834

    Article  CAS  Google Scholar 

  24. Zhan S, Wu Y, Wang L, Zhan X, Zhou P. Biosens Bioelectron, 2016, 86: 353–368

    Article  CAS  Google Scholar 

  25. Zhan S, Xu H, Zhang D, Xia B, Zhan X, Wang L, Lv J, Zhou P. Biosens Bioelectron, 2015, 72: 95–99

    Article  CAS  Google Scholar 

  26. Li T, Dong S, Wang E. Anal Chem, 2009, 81: 2144–2149

    Article  CAS  Google Scholar 

  27. Li L, Li B, Qi Y, Jin Y. Anal Bioanal Chem, 2009, 393: 2051–2057

    Article  CAS  Google Scholar 

  28. Li T, Wang E, Dong S. Anal Chem, 2010, 82: 1515–1520

    Article  CAS  Google Scholar 

  29. Wang F, Lu CH, Willner I. Chem Rev, 2014, 114: 2881–2941

    Article  CAS  Google Scholar 

  30. Liu J, Lu Y. J Am Chem Soc, 2003, 125: 6642–6643

    Article  CAS  Google Scholar 

  31. Ren W, Zhang Y, Chen HG, Gao ZF, Li NB, Luo HQ. Anal Chem, 2016, 88: 1385–1390

    Article  CAS  Google Scholar 

  32. Miyake Y, Togashi H, Tashiro M, Yamaguchi H, Oda S, Kudo M, Tanaka Y, Kondo Y, Sawa R, Fujimoto T, Machinami T, Ono A. J Am Chem Soc, 2006, 128: 2172–2173

    Article  CAS  Google Scholar 

  33. Wu Y, Zhan S, Xu L, Shi W, Xi T, Zhan X, Zhou P. Chem Commun, 2011, 47: 6027–6029

    Article  CAS  Google Scholar 

  34. Torigoe H, Ono A, Kozasa T. Chem Eur J, 2010, 16: 13218–13225

    Article  CAS  Google Scholar 

  35. Liu X, Tang Y, Wang L, Zhang J, Song S, Fan C, Wang S. Adv Mater, 2007, 19: 1471–1474

    Article  CAS  Google Scholar 

  36. Yang S, Wu C, Tan H, Wu Y, Liao S, Wu Z, Shen G, Yu R. Anal Chem, 2013, 85: 14–18

    Article  CAS  Google Scholar 

  37. Chen GH, Chen WY, Yen YC, Wang CW, Chang HT, Chen CF. Anal Chem, 2014, 86: 6843–6849

    Article  CAS  Google Scholar 

  38. Zhu S, Zhuo Y, Miao H, Zhong D, Yang X. Luminescence, 2015, 30: 631–636

    Article  CAS  Google Scholar 

  39. Lin L, Liu Y, Zhao X, Li J. Anal Chem, 2011, 83: 8396–8402

    Article  CAS  Google Scholar 

  40. Kwok RTK, Leung CWT, Lam JWY, Tang BZ. Chem Soc Rev, 2015, 44: 4228–4238

    Article  CAS  Google Scholar 

  41. Lou X, Zhao Z, Tang BZ. Small, 2016, 12: 6430–6450

    Article  CAS  Google Scholar 

  42. Mei J, Hong Y, Lam JWY, Qin A, Tang Y, Tang BZ. Adv Mater, 2014, 26: 5429–5479

    Article  CAS  Google Scholar 

  43. Mei J, Leung NLC, Kwok RTK, Lam JWY, Tang BZ. Chem Rev, 2015, 115: 11718–11940

    Article  CAS  Google Scholar 

  44. Lou X, Zhuang Y, Zuo X, Jia Y, Hong Y, Min X, Zhang Z, Xu X, Liu N, Xia F, Tang BZ. Anal Chem, 2015, 87: 6822–6827

    Article  CAS  Google Scholar 

  45. Zhuang Y, Zhang M, Chen B, Duan R, Min X, Zhang Z, Zheng F, Liang H, Zhao Z, Lou X, Xia F. Anal Chem, 2015, 87: 9487–9493

    Article  CAS  Google Scholar 

  46. Zhuang Y, Huang F, Xu Q, Zhang M, Lou X, Xia F. Anal Chem, 2016, 88: 3289–3294

    Article  CAS  Google Scholar 

  47. Cheng Y, Huang F, Min X, Gao P, Zhang T, Li X, Liu B, Hong Y, Lou X, Xia F. Anal Chem, 2016, 88: 8913–8919

    Article  CAS  Google Scholar 

  48. Min X, Zhang M, Huang F, Lou X, Xia F. ACS Appl Mater Interfaces, 2016, 8: 8998–9003

    Article  CAS  Google Scholar 

  49. Min X, Zhuang Y, Zhang Z, Jia Y, Hakeem A, Zheng F, Cheng Y, Tang BZ, Lou X, Xia F. ACS Appl Mater Interfaces, 2015, 7: 16813–16818

    Article  CAS  Google Scholar 

  50. Ou X, Hong F, Zhang Z, Cheng Y, Zhao Z, Gao P, Lou X, Xia F, Wang S. Biosens Bioelectron, 2017, 89: 417–421

    Article  CAS  Google Scholar 

  51. Xu X, Zhao W, Gao P, Li H, Feng G, Zhao Z, Lou X. NPG Asia Mater, 2016, 8: e234

    Google Scholar 

  52. Xu X, Hou R, Gao P, Miao M, Lou X, Liu B, Xia F. Anal Chem, 2016, 88: 2386–2391

    Article  CAS  Google Scholar 

  53. Tang L, Liu Y, Ali MM, Kang DK, Zhao W, Li J. Anal Chem, 2012, 84: 4711–4717

    Article  CAS  Google Scholar 

  54. Mercury update: impact on fish advisories. EPA Fact Sheet EPA-823-F-01-011. Washington DC: EPA Office of Water, 2001. 1–10

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (21525523, 21574048, 21375042, 21405054), the National Basic Research Program of China (2015CB932600, 2013CB933000), the Special Fund for Strategic New Indus-try Development of Shenzhen, China (JCYJ20150616144425376) and 1000 Young Talent (to Fan Xia).

Author information

Authors and Affiliations

  1. Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, China

    Xiaowen Ou, Xiaoding Lou & Fan Xia

  2. Shenzhen Institute of Huazhong University of Science and Technology, Shenzhen, 518000, China

    Fan Xia

Authors
  1. Xiaowen Ou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaoding Lou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fan Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xiaoding Lou or Fan Xia.

Electronic supplementary material

11426_2017_9032_MOESM1_ESM.docx

A Highly Sensitive DNA-AIEgen-Based “Turn-On” Fluorescence Chemosensor for Amplification Analysis of Hg2+ Ions in Real Samples and Living Cells

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ou, X., Lou, X. & Xia, F. A highly sensitive DNA-AIEgen-based “turn-on” fluorescence chemosensor for amplification analysis of Hg2+ ions in real samples and living cells. Sci. China Chem. 60, 663–669 (2017). https://doi.org/10.1007/s11426-017-9032-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-017-9032-x

Keywords

Search

Navigation