Screening of promising chemotherapeutic candidates from plants extracts

Over the course of our studies investigating anti-proliferative properties of compounds originating from plants against human gastric adenocarcinoma (MK-1), human uterine carcinoma (HeLa), murine melanoma (B16F10), and two human T cell lymphotropic virus type 1 (HTLV-1)-infected T-cell lines (MT-1 and MT-2), we have screened 582 extracted samples obtained from a variety of parts from 370 plants. A few extracts showed anti-proliferative activity against all cell lines, but upon further investigation, toxicity toward selected cell lines was recognized. After activity-guided fractionation, isolation of the active principles was achieved. Structure–activity relationship studies identified the components and functionalities responsible for the specific selectivity against each cancer cell line. The effect of polyacetylenes against MK-1 cells was more potent than against HeLa and B16F10 cells. The compound having a 3,4-dihydroxyphenethyl group also showed an anti-proliferative effect against B16F10 cells. Some 6-methoxyflavone derivatives and 8-hydroxy furanocoumarins were good inhibitors of HeLa cell growth. The 17 compounds whose EC50 values were less than 1 nM did not show specific cellular selectivity. Because the cytotoxic effect of 24, 25-dihydrowithanolide D toward control cells was observed at a concentration about 100 times higher than those for the cancer cell lines, withanolide was identified as the most promising chemotherapeutic candidate in our experiments.


Introduction
Development of anti-neoplastic drugs is the focus of numerous research programs around the world. Plants are the richest source of novel chemical compounds and in fact, many natural product-derived compounds have been identified as chemotherapeutic candidates [1]. For instance, vinca alkaloids, podophyllotoxins, taxanes, and camptothecins are four main classes of compounds that are wellknown anti-neoplastic drugs originating from plants [2]. It is significant that over 60 % of the currently used antineoplastic drugs are derived from natural sources including plants [3].
Over the course of our studies investigating the antiproliferative characteristics of compounds originating from plants against human gastric adenocarcinoma (MK-1), human uterine carcinoma (HeLa), murine melanoma (B16F10), and two human T-cell lymphotropic virus type 1 (HTLV-1)-infected T-cell lines (MT-1 and MT-2), we have already reported many compounds active against cancer cell lines [4]. Herein, we report not only the screening results against the above cell lines but also the active principles and analysis of their structure-activity relationships.

Screening results
The 582 samples obtained from a variety of plant parts from 370 plants (302 genera, 104 families) were extracted with MeOH under reflux. The anti-proliferative effects of the extracts against the MK-1, HeLa, B16F10, MT-1, and MT-2 cell lines were evaluated ( Table 1). The extracts listed in Table 1 are classified in the Angiosperm Phylogeny Group III system. The extracts of the leaves of Annona squamosa (Annonaceae), the aerial parts and roots of Tylophora tanakae (Asclepiadaceae), and the leaves of Thuja occidentalis (Cupressaceae) showed the most potent anti-proliferative activities against all cell lines. The extracts of the leaves of Annona cherimola (Annonaceae), the fresh leaves of Tylophora ovata and T. ovata var. brownii (Asclepiadaceae), twigs of T. ovata, the roots of Saussurea lappa (Asteraceae), the seeds of Luffa acutangula (Cucurbitaceae), the leaves of Juniperus rigida (Cupressaceae), the woods of Haematoxylum brasileto (Fabaceae), the rhizomes of Coptis japonica (Ranunculaceae), the roots of Ruta graveolens (Rutaceae), and the leaves of Cephalotaxus harringtonia (Taxaceae) showed decreasing levels of potency in the order listed. Homoharringtonine (Omacetaxine), a protein translation inhibitor for the treatment of chronic myelogenous leukemia, is found in the leaves of C. harringtonia [5]; therefore, the extract might show potent activity. There were a few extracts that had anti-proliferative activity against all cell lines and upon further investigation, toxicity toward selected cell lines was identified. Table 2 shows a summary of the sensitivity of the plant extracts toward MK-1, HeLa, B16F10, MT-1, and MT-2 cells. The percentage of extracts that were active at concentrations of less than 100 lg/mL against the various cell lines were as follows: B16F10 (70 %), MK-1 (55 %), HeLa (39 %), MT-1 (23 %), and MT-2 (28 %). Adult T-cell leukemia/lymphoma (ATL) is a malignancy of mature peripheral T lymphocytes caused by HTLV-1. Although conventional chemotherapeutic regimens used against other malignant lymphomas have been administered to ATL patients, the therapeutic outcomes remain very poor. Therefore, these results suggest that a few plant extracts were sensitive to the T-cell lymphotropic virus type 1 (HTLV-1)-infected T cells (MT-1 and MT-2).
Active principles Polyacetylenes ( Fig. 1) After activity-guided fractionation against MK-1 cells, two active polyacetylenes, falcarindiol (1) and panaxynol (2), were isolated from the roots of Heracleum moellendorffii (Apiaceae) [6]. Six other polyacetylenes were isolated from the roots of Angelica japonica (Apiaceae) [7] together with 1 and 2 after activity-guided fractionation against MK-1 cells. Among them, four compounds were furanocoumarin ethers of 1. It was evident that the effects of these compounds except for compound 3 against MK-1 cells were more potent than their effects against HeLa and B16F10 cells (Table 3). Because compound 2 showed 16 times greater activity when compared with its 8-hydroxy derivative (1), the presence of a hydroxy group at C-8 was presumed to reduce activity. The most potent compound was panaxynol (2), with an EC 50 value of 1.2 lM against MK-1 cells. Bioactive panaxynol-type polyacetylenes in plant-derived foods have attracted attention as health-promoting compounds [8].

Lignans (Fig. 2)
After activity-guided fractionation against MK-1, HeLa, and B16F10 cells, seven lignans including deoxypodophyllotoxin (9), (-)-deoxypodorhizone (10), and related compounds were isolated from the roots of Anthriscus sylvestris (Apiaceae) [9]. From the fruits of the same plant, two other lignans (14 and 15) were isolated together with 9 and 10 after activity-guided fractionation against MK-1, HeLa, and B16F10 cells [10]. Deoxypodophyllotoxin (9) showed higher activity than polyacetylenes against these cell lines. Etoposide, a clinically used chemotherapeutic agent against small-cell lung cancer, malignant lymphoma, and acute leukemia is a derivative of a podophyllotoxin isolated from Podophyllum peltatum (Berberidaceae) [11]. Of note is that the EC 50 value of deoxypodophyllotoxin (9) was in the nanomolar range across all cell lines tested including MT-1 and MT-2 cells (Table 3). Topoisomerase II-inhibited DNA breakage was recognized as the mechanism of action of Etoposide. The structural features that are crucial for the anti-topoisomerase II activity of podophyllotoxin derivatives have been roughly identified as: bulky 7b-bulky substituent, trans-lactone in ring D, dioxolane ring in ring A, quasi-axial configuration of ring E, and 4 0hydroxy group [12].

Tagetes patula
Aerial parts

Paederia scandens
Fruits  Because the effects of compound 23 were extremely weak, this suggested the 3,4-dihydroxyphenethyl group is essential for the observed strong anti-proliferative activity. Furthermore, 3,4-dihydroxyphenethyl alcohol itself showed potent activity [13]. It is also known that treatment of phenylethanoids resulted in apoptotic cell death [16].

Polyphenols (Fig. 4)
Epidemiological studies have suggested that the consumption of green tea [Camellia sinensis (Theaceae)] provides protection against stomach cancer. In a rural area of northern Kyushu, Japan, a decreased risk of stomach cancer was also noted among people reporting a high consumption of green tea [17]. Fractionation of green tea extract, guided by the anti-proliferative activity against MK-1 cells, resulted in the isolation of six flavan-3-ols (24)(25)(26)(27)(28)(29) together with the inactive glycosides of kaempferol and quercetin [18]. A study of their structure-activity relationships suggested that the presence of the three adjacent hydroxyl groups (pyrogallol or galloyl group) in the molecule is a key factor for enhancing the compound's activity. Six active polyphenols (30)(31)(32)(33)(34)(35) were isolated from the seeds of Rhynchosia volubilis (Fabaceae) after activityguided fractionation against MK-1, HeLa, and B16F10 cells [19]. These compounds all showed much stronger inhibition against B16F10 cell growth than against HeLa and MK-1 cell growth. Gallic acid (31) with a free carboxyl group showed higher activity than its methyl ester (32). A hydrolysable tannin (36) and two condensed tannins (37,38) isolated from Phyllanthus emblica (Phyllanthaceae) also showed potent activity [20] against three cell lines. It was proposed that the anti-cancer properties of polyphenols may be related to their ability to participate in a copper-dependent prooxidant mechanism [21].

Curcuma zedoaria
Sesquiterpenes (Fig. 6) After activity-guided fractionation against MK-1, HeLa, and B16F10 cells, five active sesquiterpenes (60-64) were isolated from the roots of Inula helenium (Asteraceae) together with an inactive sesquiterpene (65) and a weak one (66) [24]. A structure-activity study suggested that the presence of an a-methylene-c-lactone group is a key component required for the anti-proliferative activity. The thiol reactivity of the a-methylene-c-lactone group may be responsible for the observed anti-proliferative activity [25]. Two norsesquiterpene glycosides from the roots of Phyllanthus emblica (Phyllanthaceae) exhibited potent activity (data not shown) although their aglycone and monoglucoside showed no inhibitory activity [20].
Triterpene glycosides (Fig. 7) and triterpenes (Fig. 8) From the bioactive fraction of the fruits of Bupleurum rotundifolium (Apiaceae), ten ursane-type triterpene glycosides were isolated and their anti-proliferative activities against MK-1, HeLa, and B16F10 cells were estimated [26]. All active glycosides (67-71) have a 13b, 28-epoxy The percentages for each cell type represent the percent of the extracts with EC 50 s within each category of activity     oleanane-type triterpene glycosides were also isolated and their anti-proliferative activities were evaluated [27]. Similar to the ursane-type triterpene glycosides, all active glycosides (74-78) have a 13b, 28-epoxy ring system in the molecule. In contrast to the ursane-type triterpene glycosides, compounds 77 and 78, which have a 21a-hydroxy group, had potent anti-proliferative activities. The configuration at the C-21 hydroxy group might influence the antiproliferative activity. After activity-guided fractionation against MK-1, HeLa and B16F10 cells, ten triterpenes were isolated from the aerial parts of Centella asiatica (Apiaceae) [28]. Some (79-84) of these triterpenes showed potent anti-proliferative activities. Similar to the results of the polyphenols, ursolic acid (80) with a free carboxyl group showed higher activity than its lactone (79). Ursolic acid (80) was previously reported to induce apoptoic cell death [29].
Coumarins and acridone alkaloids ( Fig. 9) After activity-guided fractionation against MK-1, HeLa, and B16F10 cells, 16 compounds were isolated from the aerial parts and roots of Boenninghausenia japonica (Rutaceae) [30]. Among them, an acridone alkaloid (91) showed very strong anti-proliferative activity against these three cell lines. The EC 50 value of 91 was in the nanomolar range except for against B16F10 cells. Therefore, a 3 0 , 4 0epoxy group might be important for enhancing the antiproliferative activity of acridone alkaloids. Furthermore, some furanocoumarins (85-90) showed potent anti-proliferative activities against HeLa cells. The furanocoumarins (87, 88) having an 8-hydroxy group showed more potent activity than those without the substituent (85, 86) and those with an 8-methoxy group (89, 90) against HeLa cells. Therefore, an 8-hydroxy group may be important for enhancing the anti-proliferative activity of these compounds against HeLa cells. Some furanocoumarins (85, 86,  (Table 3). A recent review reports that natural and synthetic coumarins have anticancer activity toward various cell lines [31].

Withanolides (Fig. 10)
After activity-guided fractionation against MT-1 and MT-2 cells, five active withanolides (93-97) were isolated from the aerial parts of Physalis pruinosa (Solanaceae) [32]. Structure-activity relationships suggested that the presence of a 5b, 6b epoxy group in the B-ring and a 4b-hydroxy group in the A-ring were important for the observed activities. The aliphatic ether side chain at C-5 also seems to increase the activity because as the side chain is lengthened, the activity increases. Because the EC 50 value for 4b-hydroxywithanolide E (93) was in the nanomolar range against both MT-1 and MT-2 cells, 31 other withanolides were also evaluated [33]. Except for compound 98, none of the glycosides showed any activity against the ATL cell lines. Because compound 98 has a 5b, 6b-epoxy group as well as a 4b-hydroxy group, we predicted it might show potent activity. However, the activity of the corresponding deglycosylated compound (99) was approximately four times greater than that of its glucoside (98). These results indicated that the presence of a sugar moiety should reduce the anti-proliferative effects. The importance of the 5b, 6b-epoxy group, and 4b-hydroxy group was further supported by the analysis of compound 100 because it showed the second strongest anti-proliferative activity. The activities of 99, having a double bond between C-2 and C-3, were weaker than those for 100, suggesting the double bond between C-2 and C-3 might reduce the activity.
Because compound 102 showed greater activity when compared with compound 103, the importance of a 4bhydroxy group was further supported. Compared with the compounds having the 5b, 6b-epoxy group, the activities of the compounds (104-106) having other types of epoxy groups (6a, 7a-epoxy group in the B-ring and/or 24a, 25aepoxy group in the E-ring) were significantly lower. The position of the epoxy group and/or the configuration of the epoxy group seem to be important for the activity. Compound 106 containing a 15b-hydroxy group showed moderate activity while compound 105 containing a 15a- Fig. 4 Structures of polyphenols identified from a screen of plant extracts hydroxy group did not show any activity. This indicated that the configuration of the hydroxy group at C-15 may influence a compound's activity. Finally, the EC 50 value of 24, 25-dihydrowithanolide D (101), the most potent withanolide-type inhibitor, was 8 nM against both cells. In contrast, the cytotoxic effect (860 nM) of 101 toward normal cells was observed at a concentration about 100 times higher than those observed for the ATL cell lines. Furthermore, compound 101 was confirmed to induce dose-dependent apoptosis against MT-1, MT-2, and fresh ATL cells [33]. Therefore, 24, 25-dihydrowithanolide D (101) may be a promising chemotherapeutic candidate.
Recently inhibition of the growth of human lung cancer cells through DNA damage, apoptosis and G2/M arrest by 4b-hydroxywithanolide E (93) have been reported [34]. Further, induction of apoptosis in leukemia cells by targeting the activation of a neutral sphingomyelinase-ceramide cascade mediated by synergistic activation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase by withanolide D have been also reported [35].    Wang et al. suggested that Hsp90 inhibition by the withanolides is correlated with their ability to induce cancer cell death [36].
Aporphine and phenanthroindolizidine alkaloids (Fig. 11) After activity-guided fractionation against MT-1 and MT-2 cells, three active aporphine alkaloids (107-109) were isolated from the leaves of Annona reticulata and A. squamosa (Annonaceae) [37]. Liriodenine (107) showed accumulation of Sub-G1 stage cells in the MT-1 and MT-2 cell population, suggesting induction of apoptosis. A structure-activity relationship analysis suggested that the presence of a 1, 2-methylenedioxy group seemed to enhance activity. A similar conclusion on the structure-activity relationship was also obtained by Liu et al. [38]. Six phenanthroindolizidine alkaloids (110-115) were isolated from the aerial parts of Tylophora tanakae (Asclepiadaceae) by activity-guided fractionation [39]. In addition to 110-115, three phenanthroindolizidine alkaloids (116-118) obtained from other plants were examined for their anti-proliferative activity against MT-1 and MT-2 cells. The EC 50 values of all alkaloids except for compound 113 were in the low nanomolar range. The results suggested that the presence of a 2-methoxy functionality, the methyl group of a 7-methoxy functionality, and an N-oxide moiety appear to reduce the potency of the anti-proliferative activity [39]. Phenanthroindolizidine alkaloids are cytotoxic to multidrug-resistant cells [40], inhibiting the enzyme dihydrofolate reductase [41]. The in vivo efficacy of a new phenanthroindolizidine alkaloid derivative (YPC-10157) was recently evaluated [42].
The compounds whose EC 50 values were less than one nanomolar (\1 nM) were not selective for specific cell types. This group included two lignans (9,15), one acridone alkaloid (91), six withanolides (93, 98-102), and eight phenanthroindolizidine alkaloids (110-112, 114-118). Because the cytotoxic effect of 24, 25-dihydrowithanolide D (101) toward normal cells was observed at a concentration about 100 times higher than against the ATL cell lines, withanolide was concluded to be the most promising chemotherapeutic candidate from our experiments.