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PbO nanoparticles increase the expression of ICAM-1 and VCAM-1 by increasing reactive oxygen species production in choroid plexus

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Abstract

PbO nanoparticles (nano-PbO) are widely used in the production of electrode materials, but exposure to them can cause brain damage. The first barrier preventing nano-PbO from entering the brain is the choroid plexus. However, the effect of nano-PbO on the choroid plexus remains unclear. Thus, the purpose of this study was to investigate the effect of nano-PbO exposure on lymphocyte cells infiltration, the adhesion protein of the choroid plexus as well as the role of reactive oxygen species (ROS) during the process. Results showed that nano-PbO exposure increased the percentage of lymphocyte cells in the brain and upregulated the expression of surface adhesion proteins, including intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in choroid plexus. Meanwhile, nano-PbO treatment also resulted in the increase of intercellular ROS production, and significantly decrease glutathione (GSH) content, glutathione peroxidase (GSH-PX) activity, and superoxide dismutase (SOD) activity in Z310 cells beside the increase of ICAM and VCAM-1 expression. Treatment with ROS inhibitor N-acetylcysteine (NAC) significantly downregulated the expression of ICAM-1 and VCAM-1expression. In conclusion, exposure to nano-PbO increases the expression of ICAM-1 and VCAM-1 through oxidative stress, which may contribute to peripheral lymphocyte cells infiltration into the brain.

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Funding

This work was supported by the Science and Technology Project of Hebei Education Department (ZD2020113), National Natural Science Foundation of China (82073598), and Natural Science Foundation of Hebei Province (H2020209250).

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Authors and Affiliations

  1. School of Public Health, North China University of Science and Technology, Tangshan, China

    Weixuan Wang, Shuang Li, Xi Wang, Jianbo Wang & Yanshu Zhang

  2. The Experiment Animal Center, North China University of Science and Technology, Tangshan, China

    Shuang Li & Yanshu Zhang

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  1. Weixuan Wang
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Contributions

YS Zhang and S Li designed this study; WX Wang and S Li interpreted the experimental results; X Wang, WX Wang, and JB Wang performed the experiments; X Wang, WX Wang, and S Li wrote the manuscript; and YS Zhang and WX Wang approved the final version of this paper.

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Correspondence to Yanshu Zhang.

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Wang, W., Li, S., Wang, X. et al. PbO nanoparticles increase the expression of ICAM-1 and VCAM-1 by increasing reactive oxygen species production in choroid plexus. Environ Sci Pollut Res 30, 40162–40173 (2023). https://doi.org/10.1007/s11356-022-25109-8

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