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LncRNA MAFG-AS1-induced acute myeloid leukemia development via modulating miR-147b/HOXA9

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Abstract

Recent references discovered that lncRNAs acted roles in malignant cancer development. However, the role of MAFG-AS1 in acute myeloid leukemia (AML) development remains unknown. MAFG-AS1 and miR-147b were determined in AML cells and specimens using qRT-PCR assay. Cell proliferation was detected by CCK-8 analysis and flow cytometry was carried out to measure cell cycle. Luciferase reporter analysis was done to determine the mechanism of MAFG-AS1 and miR-147b. We noted that MAFG-AS1 was overexpressed in AML cells and in serum and bone narrow from AML compared with normal controls specimen. Elevated expression of MAFG-AS1 increased cell growth, cycle and EMT in AML cell HL-60 cell. MAFG-AS1 sponged miR-147b expression in HL-60 cell. Moreover, miR-147b was downregulated in AML cells and in serum and bone narrow from AML compared with normal control specimen. miR-147b was negatively correlated with MAFG-AS1 in the serum and bone narrow of AML cases. We illustrated that HOXA9 was one target of miR-147b and ectopic expression of MAFG-AS1 enhanced HOXA9 expression HL-60 cell. Forced expression of MAFG-AS1 induced cell growth, cycle, and EMT via promoting HOXA9. These data illustrated that MAFG-AS1 acted as one oncogenic gene and accelerated AML progression via modulating miR-147b/HOXA9 axis.

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Authors and Affiliations

  1. College of Basic Medical Sciences, Dalian Medical University, Dalian, 116027, Liaoning, China

    Qiying Yao

  2. Department of Pediatrics, The Second Hospital of Dalian Medical University, Dalian, 116023, Liaoning, China

    Li Zhang, Zhengjuan Liu, Yuchuan Wang, Junli Liu & Yingjie Wang

  3. Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, 150001, Heilongjiang, China

    Lei Yu

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  1. Qiying Yao
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  2. Li Zhang
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  3. Zhengjuan Liu
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  4. Lei Yu
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  5. Yuchuan Wang
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  6. Junli Liu
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  7. Yingjie Wang
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Contributions

Qiying Yao, Li Zhang, Zhengjuan Liu, Lei Yu, Yuchuan Wang, Junli Liu, and Yingjie Wang designed and conceived the experiments, performed the data analysis, drafted the original manuscript, and revised manuscript.

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Correspondence to Yingjie Wang.

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Responsible editor: Lotfi Aleya

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Yao, Q., Zhang, L., Liu, Z. et al. LncRNA MAFG-AS1-induced acute myeloid leukemia development via modulating miR-147b/HOXA9. Environ Sci Pollut Res 30, 19250–19258 (2023). https://doi.org/10.1007/s11356-022-23537-0

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