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Laboratory and field studies on the combined application of Beauveria bassiana and fipronil against four major stored-product coleopteran insect pests

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Abstract

In the current study we have tested the application of Beauveria bassiana (Hypocreales: Cordycipitaceae) alone and in combination with fipronil at two doses against Tribolium castaneum (Coleoptera: Tenebrionidae), Rhyzopertha dominica (Coleoptera: Bostrychidae), Sitophilus granarius (Coleoptera: Curculionidae), and Trogoderma granarium (Coleoptera: Dermestidae) under laboratory and field conditions. At laboratory conditions, the combination of B. bassiana with the highest dose of fipronil produced the highest mortality. At different temperatures, mortality was increased with the increase in temperature. Maximum mortality was observed at 30 °C, followed by 25 °C and 20 °C for all tested species. Different treatments significantly reduced the progeny number in comparison to control groups for all tested species at all temperatures. In the persistence trial, all treatments that included the combinations of B. bassiana with fipronil produced significantly higher mortalities than the single treatments for all tested species over a period of 6 months. Furthermore, all treatments significantly reduced the number of progenies of all insect species in comparison with the control groups over the same storage period. In field trials, mortalities of all tested insect species were significantly higher on wheat treated with B. bassiana, fipronil, or their combinations than on controls for an entire storage period of 180 days. Overall, R. dominica was found the most susceptible species followed by S. granarius, T. castaneum, and T. granarium. The findings of the current study suggest that the use of B. bassiana and fipronil as grain protectants may provide elevated control against major stored-grain insect species during a prolonged period of storage.

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Data availability

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was funded by the Agricultural Linkages Program CS-097, Pakistan Agricultural Research Council (ALP-PARC), Islamabad, Pakistan.

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Authors and Affiliations

  1. Department of Entomology, University of Agriculture, Faisalabad, 38040, Pakistan

    Waqas Wakil & Muhammad Usman

  2. Senckenberg German Entomological Institute, Eberswalder str. 90, 15374, Müncheberg, Germany

    Waqas Wakil

  3. Laboratory of Agricultural Zoology and Entomology, Department of Crop Science, Agricultural University of Athens, 75 Iera Odos str, 11855, Athens, Attica, Greece

    Nickolas G. Kavallieratos

  4. Department of Plant Pathology, College of Agriculture, University of Sargodha, Sargodha, 40100, Pakistan

    Muhammad Usman Ghazanfar

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  1. Waqas Wakil
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  2. Nickolas G. Kavallieratos
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  3. Muhammad Usman Ghazanfar
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  4. Muhammad Usman
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Correspondence to Waqas Wakil or Nickolas G. Kavallieratos.

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Wakil, W., Kavallieratos, N.G., Ghazanfar, M.U. et al. Laboratory and field studies on the combined application of Beauveria bassiana and fipronil against four major stored-product coleopteran insect pests. Environ Sci Pollut Res 29, 34912–34929 (2022). https://doi.org/10.1007/s11356-021-17527-x

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  • DOI: https://doi.org/10.1007/s11356-021-17527-x

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