Abstract
Intracellular phosphorus (P) accumulation can improve microalgal growth and lipid synthesis. However, large excess of P causes cell poisoning. This study utilized a P-fed-batch strategy to investigate its potential to improve the utilization of the excessive P, while avoiding toxic side effects. This strategy contributed to a more complete utilization of the intracellularly stored P, which enhanced the microalgae biomass by 10−15% by upregulating the brassinosteroid growth hormone gene at a P-fed-batch frequency of 2−8. Furthermore, the lipid content increased by 4−16% via upregulation of lipid synthesis-related genes. As a result, the P-fed-batch strategy significantly increased the lipid production by 13−19%. The content of saturated fatty acid increased by ~ 100%, implying improved combustibility and oxidative stability. This is the first study of this P-fed-batch strategy and provides a new concept for the complete utilization of excessive P.
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Funding
This research was financially supported by the National Natural Science Foundation of China (51708095, 51578117, 51722803), Fundamental Research Funds for the Central Universities (2412017QD027, 2412018ZD013, 2412018ZD042), the Science and Technology Project of Jilin Province (20180520168JH), and China Postdoctoral Science Foundation (2017M611302, 2018T110241).
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Fu, L., Yan, G., Li, Y. et al. Phosphorus supply via a fed-batch strategy improves lipid heterotrophic production of Chlorella regularis. Environ Sci Pollut Res 27, 31677–31685 (2020). https://doi.org/10.1007/s11356-020-09495-5
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DOI: https://doi.org/10.1007/s11356-020-09495-5