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Cu/Zn superoxide dismutase (SOD) and catalase (CAT) response to crude oil exposure in the polychaete Perinereis aibuhitensis

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Abstract

Cu/Zn superoxide dismutase (SOD) and catalase (CAT) cDNAs from the polychaete Perinereis aibuhitensis were cloned and characterized in order to investigate the relationship between crude oil exposure and stress response in this worm. The full length of PaSOD was 870 bp and PaCAT was 1967 bp encoding 150 and 506 amino acids, respectively. Gene expression and enzyme activity of Cu/Zn SOD and CAT in response to crude oil contaminated soil (500, 1500, and 3000 mg/kg) were measured. The results showed that expression of the CAT gene and enzyme activity in P. aibuhitensis was positively correlated to the concentration of crude oil and reached a maximum at 15 days of exposure to 3000 mg/kg crude oil. The expression of the SOD gene and enzyme activity of SOD in P. aibuhitensis also increased during exposure to crude oil and reached a maximum at 10 days of exposure to 3000 mg/kg crude oil. These results indicated that SOD and CAT are important for maintaining the balance of cellular metabolism and protecting P. aibuhitensis from crude oil toxicity.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 41306138), the National Marine Public Welfare Research Project (Nos. 201305002, 201305043), and the Project of Marine Ecological Restoration Technology Research to the Penglai 19-3 Oil Spill Accident (No. 19-3YJ09).

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Authors and Affiliations

  1. Key Laboratory of Marine Bio-Resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, No. 52, HeiShiJiao Street, Dalian, 116023, Liaoning, People’s Republic of China

    Huan Zhao, Wanjuan Li, Xinda Zhao, Dazuo Yang, Hongwei Ren & Yibing Zhou

  2. Dalian Productivity Promotion Center, Dalian Ocean University, Dalian, 116025, People’s Republic of China

    Xu Li

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  1. Huan Zhao
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  2. Wanjuan Li
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  4. Xu Li
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Correspondence to Yibing Zhou.

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Responsible editor: Cinta Porte

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Zhao, H., Li, W., Zhao, X. et al. Cu/Zn superoxide dismutase (SOD) and catalase (CAT) response to crude oil exposure in the polychaete Perinereis aibuhitensis . Environ Sci Pollut Res 24, 616–627 (2017). https://doi.org/10.1007/s11356-016-7594-0

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