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A biosurfactant-producing yeast Rhodotorula sp.CC01 utilizing landfill leachate as nitrogen source and its broad degradation spectra of petroleum hydrocarbons

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Abstract

Biosurfactants (BSs) are known for their remarkable properties, however, their commercial applications are hampered partly by the high production cost. To overcome this issue, a biosurfactant producing strain, Rhodotorula sp.CC01 was isolated using landfill leachate as nitrogen source, while olive oil was determined as the best sole carbon source. The BS produced by Rhodotorula sp.CC01 had oil displacement diameter of 19.90 ± 0.10 cm and could reduce the surface tension of water to 34.77 ± 0.63 mN/m. It was characterized as glycolipids by thin layer chromatography, FTIR spectra, and GC–MS analysis, with the critical micelle concentration of 70 mg/L. Meanwhile, the BS showed stability over a wide range of pH (2–12), salinity (0–100 g/L), and temperature (20–100 °C). During the cultivation process, BS was produced with a maximum rate of 163.33 mg L−1 h−1 and a maximum yield of 1360 mg/L at 50 h. In addition, the removal efficiency of NH4+–N reached 84.2% after 75 h cultivation with a maximum NH4+–N removal rate of 3.92 mg L−1 h−1. Moreover, Rhodotorula sp.CC01 has proven to be of great potential in remediating petroleum hydrocarbons, as revealed by chromogenic assays. Furthermore, genes related to nitrogen metabolism and glycolipid metabolism were found in this strain CC01 after annotating the genome data with KEGG database, such as narB, glycoprotein glucosyltransferase, acetyl-CoA C-acetyltransferase, LRA1, LRA3, and LRA4. The findings of this study prove a cost-effective strategy for the production of BS by yeast through the utilization of landfill leachate.

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Fig. 1
Fig. 2

source for BS production by strain CC01. b Kinetics of growth and ST of strain CC01 during 243 h of incubation. The ST decreased to 28.20 ± 0.51 mN/m at 15 h and remained stable and biomass reached a maximum at 147 h. The error bars represent the standard deviations (n = 3)

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Acknowledgements

This study was financially supported by Zhoushan Science and Technology Department Project (Grant No. 2019C81056), the National Natural Science Foundation of China (Grant Nos. 51978189; 52070050), and by the Research Funds of the Guangxi Key Laboratory of Theory and technology for Environmental Pollution Control (Grant No. 2001K004).

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  1. Chunfang Zhang and Qinglin Xie have contributed equally to this article and are joint corresponding authors.

Authors and Affiliations

  1. Institute of Marine Biology, Ocean College, Zhejiang University, Zhoushan, 316021, Zhejiang, China

    Xiaoyun Lin, Hanghai Zhou, Lijia Jiang, Edidiong Okokon Atakpa, Gen Chen & Chunfang Zhang

  2. College of Environmental Science and Engineering, Guilin University of Technology, Guilin, 541006, China

    Feng Zeng & Qinglin Xie

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  1. Xiaoyun Lin
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Contributions

XL: Conceptualization, Methodology, Investigation, Formal analysis, Data curation, Writing—original draft; HZ: Investigation, Formal analysis, Data curation; FZ: Investigation; LJ: Investigation; EOA: Manuscript revision; GC: Data curation; CZ: Validation, Writing—review and editing, Project administration; QX: Review and editing.

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Correspondence to Chunfang Zhang or Qinglin Xie.

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Lin, X., Zhou, H., Zeng, F. et al. A biosurfactant-producing yeast Rhodotorula sp.CC01 utilizing landfill leachate as nitrogen source and its broad degradation spectra of petroleum hydrocarbons. World J Microbiol Biotechnol 38, 68 (2022). https://doi.org/10.1007/s11274-022-03254-z

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