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RETRACTED ARTICLE: A method of multiplex PCR for detection of field released Beauveria bassiana, a fungal entomopathogen applied for pest management in jute (Corchorusolitorius)

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This article was retracted on 28 May 2020

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Abstract

The fungal entomopathogen Beauveria bassiana is a promising biocontrol agent for many pests. Some B. bassiana strains have been found effective against jute pests. To monitor the survival of field released B. bassiana a rapid and efficient detection technique is essential. Conventional methods such as plating method or direct culture method which are based on cultivation on selective media followed by microscopy are time consuming and not so sensitive. PCR based methods are rapid, sensitive and reliable. A single primer PCR may fail to amplify some of the strains. However, multiplex PCR increases the possibility of detection as it uses multiple primers. Therefore, in the present investigation a multiplex PCR protocol was developed by multiplexing three primers SCA 14, SCA 15 and SCB 9 to detect field released B. bassiana strains from soil as well as foliage of jute field. Using our multiplex PCR protocol all the five B. bassiana strains could be detected from soil and three strains viz., ITCC 6063, ITCC 4563 and ITCC 4796 could be detected even from the crop foliage after 45 days of spray.

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Fig. 1
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Change history

  • 28 May 2020

    The Editor-in-Chief has retracted this article [1] due to similarities between lanes 1-4 in Figures 1 and 2. These similarities have raised concerns about the reliability of the data presented. The authors, Chinmay Biswas, Piyali Dey, B. S. Gotyal & Subrata Satpathy have not responded to correspondence about this retraction. [1] Biswas, C., Dey, P., Gotyal, B.S. et al. A method of multiplex PCR for detection of field released Beauveria bassiana, a fungal entomopathogen applied for pest management in jute (Corchorus olitorius). World J Microbiol Biotechnol 31, 675 -679 (2015). https://doi.org/10.1007/s11274-015-1821-6

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Authors and Affiliations

  1. Central Research Institute for Jute and Allied Fibres (CRIJAF), Barrackpore, Kolkata, 700120, West Bengal, India

    Chinmay Biswas, Piyali Dey, B. S. Gotyal & Subrata Satpathy

Authors
  1. Chinmay Biswas
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  2. Piyali Dey
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  3. B. S. Gotyal
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  4. Subrata Satpathy
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Corresponding author

Correspondence to Chinmay Biswas.

Additional information

The Editor-in-Chief has retracted this article due to similarities between lanes 1-4 in Figures 1 and 2. These similarities have raised concerns about the reliability of the data presented. The authors, Chinmay Biswas, Piyali Dey, B. S. Gotyal & Subrata Satpathy have not responded to correspondence about this retraction.

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Biswas, C., Dey, P., Gotyal, B.S. et al. RETRACTED ARTICLE: A method of multiplex PCR for detection of field released Beauveria bassiana, a fungal entomopathogen applied for pest management in jute (Corchorusolitorius). World J Microbiol Biotechnol 31, 675–679 (2015). https://doi.org/10.1007/s11274-015-1821-6

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  • DOI: https://doi.org/10.1007/s11274-015-1821-6

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