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Potentials of single nucleotide polymorphisms and genetic diversity studies at HSP90AB1 gene in Nigerian White Fulani, Muturu, and N’Dama cattle breeds

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Abstract

The study was aimed at genetic characterization of Nigerian breeds of Muturu, N’Dama, and White Fulani cattle breeds at heat shock protein 90AB1 locus. Also, the goal of the study was to detect the presence of single nucleotide polymorphisms (SNPs) at HSP90AB1 locus and consequently recommend them as bio-markers for thermo-tolerance potentials in Nigerian cattle breeds when exposed to assaults of thermal conditions/heat shock of tropical environment. Based on the previously published potentials of this candidate gene to lower assaults of thermal conditions/heat shock such as heat stress, the detected SNPs of HSP90AB1 within the population of the Nigerian cattle in this study will be recommended for population-based screening with a view to genetically improving those zebu cattle breeds that are more vulnerable to heat shock and assaults of thermal conditions. Total number of 200 blood samples were randomly collected from White Fulani (84 samples), Muturu (73 samples), and N’Dama (43 samples) breeds of cattle. Out of these, 20 DNA samples were randomly selected from each of the three cattle breeds and were used for DNA extraction and downstream analyses to further confirm findings of previous study, hence the goal of our study. DNA was extracted from the blood samples using the Zymo-bead DNA extraction kit and DNA sequencing of our samples was performed. A total number of 9 SNPs (within exons 5–6 coding regions) and 11 SNPs (within exons 12–13 coding regions) were detected at HSP90AB1 locus using the codon code aligner software. ARLEQUIN 2.0001 software was used to estimate the basic population genetic statistics while the DnaSP version 5.10.01 was used to estimate the genetic diversity indices. This study detected new SNPs (polymorphic sites) at HSP90AB1 locus within the DNAs of Nigerian White Fulani (WF), Muturu (MU), and N’Dama (ND) breeds of cattle. Within exons 5–6 coding regions, the N’Dama (ND) cattle breed had the highest for number of SNPs (5) and genetic diversity indices while White Fulani (WF) and Muturu (MU) had the least (2) number of SNPs each. Within exons 12–13 coding regions, WF had the highest numbers of SNPs (7) and genetic diversity indices while MU had the least number of SNPs (1) and genetic diversity indices. Some of the detected SNPs at HSP90AB1 locus were shared among the three breeds, suggesting that these three Nigerian cattle breeds showed shared ancestral alleles and lineage. Our study further revealed that HSP90AB1 is highly polymorphic/variable and diverse among the three Nigerian cattle breeds examined. Based on the previously documented thermo-tolerance potentials of members of HSP90 sub-family including the findings of our study, we hypothesize therefore that the presence of SNPs of HSP90AB1 within the DNAs of these three breeds of Nigerian cattle (WF, ND, and MU) may confer them thermo-tolerance potentials for thermal assault conditions and heat shock of the tropics at HSP90AB1 locus. Therefore, the detected SNPs can be recommended as bio-markers to improve the thermo-tolerance potentials of Nigerian breeds of zebu cattle raised under the challenges of heat shock for better adaptation and survival.

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Acknowledgements

We thank the Cattle Production Venture (CPV) and Institute of Food Security Environmental Resources and Agricultural Research (IFSERAR) all located at the Federal University of Agriculture, Abeokuta, Ogun State, Nigeria, for allowing us to take blood samples from their available Cattle.

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Authors and Affiliations

  1. Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria

    John S. De Campos, Akpan Ubong, Adeyemi S. Adenaike & Christian O. Ikeobi

  2. Department of Animal Science, Federal University Dutse, Dutse, Jigawa State, Nigeria

    Gbolabo O. Onasanya

  3. Department of Animal Production, University of Ilorin, Ilorin, Kwara State, Nigeria

    Afolabi T.Yusuff

  4. Department of Agricultural Education, Federal College of Education, (Special), Oyo, Oyo State, Nigeria

    Akinfolarin A. Mohammed

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  1. John S. De Campos
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Contributions

All authors (John S. De Campos, Gbolabo O. Onasanya, Akpan, Ubong, Afolabi T.Yusuff, Adeyemi S. Adenaike, Akinfolarin A. Mohammed, Christian O. Ikeobi) contributed to the study conception and design. Material preparation, data collection, and analysis were performed by John Sunday De Campos, Onasanya Gbolabo, and Adeyemi Adenaike. The first draft of the manuscript was written by John De Campos and all authors commented on previous versions of the manuscript. Mohammed Akinfolarin Abubakar, Akpan Ubong, and Afolabi Taoheed Yusuff provided the scientific structured contents and all authors (John S. De Campos, Gbolabo O. Onasanya, Akpan, Ubong, Afolabi T.Yusuff, Adeyemi S. Adenaike, Akinfolarin A. Mohammed, Christian O. Ikeobi) read and approved the final manuscript.

Corresponding author

Correspondence to John S. De Campos.

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Ethics approval

This study was approved by the University Ethics Research Committee with approval project number of FUNAAB/AEWC/2022/0021. Animal welfare was not compromised anytime during this study and all our research protocols were cleared by the institution prior to embarking on the activities reported here.

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The authors declare no competing interests.

We declare to the editor of this journal that this is our original work and has not been submitted for publication elsewhere.

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De Campos, J.S., Onasanya, G.O., Ubong, A. et al. Potentials of single nucleotide polymorphisms and genetic diversity studies at HSP90AB1 gene in Nigerian White Fulani, Muturu, and N’Dama cattle breeds. Trop Anim Health Prod 56, 58 (2024). https://doi.org/10.1007/s11250-024-03909-z

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