Skip to main content
SpringerLink
Log in

Antimicrobial susceptibility and molecular typing of Pasteurella multocida isolated from six provinces in China

  • Short Communications
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Pasteurella multocida (P. multocida) is an important pathogen that causes bovine respiratory disease (BRD) in China and other countries. To investigate the antimicrobial susceptibility of P. multocida isolated from different provinces in China, we analyzed antimicrobial susceptibility phenotypes and pulsed-field gel electrophoresis (PFGE) types of P. multocida; then, we sequenced the complete genome of strain found to be multidrug-resistant. The isolates exhibited resistance to many antimicrobial agents, especially amikacin, sulfamethoxazole, sulfachloropyridazinesodium, macrolides, and fluoroquinolones. Pulsed-field gel electrophoresis analysis showed that a clonal spread of multidrug-resistant isolates occurred in various provinces. All of the isolates carried class I integron.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Clinical and Laboratory Standards Institute, 2013. Performance standards for antimicrobial disk dilution susceptibility tests for bacteria isolated from animals.approved standard. 4th ed, Wayne, PA: CLSI. CLSI Document VET01-A4.

  • Ewers, C., Lübke-Becker, A., Bethe, A., Kiebling, S., Filter, M. and Wieler, L.H., 2006 Virulence genotype of Pasteurella multocida strains isolated from different hosts with various disease status, Veterinary microbiology, 114, 304–317.

    Article  CAS  PubMed  Google Scholar 

  • Ferreira, T.S., Felizardo, M.R., de Gobbi, D.D., Moreno, M. and Moreno, A.M., 2015. Antimicrobial resistance and virulence gene profiles in P. multocidastrains isolated from cats, Brazilian journal of microbiology, 46, 271–277.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hendriksen, R.S., Mevius, D.J., Schroeter, A., Teale, C., Meunier, D., Butaye, P., Franco, A., Utinane, A., Amado, A., Moreno, M., Greko, C., Stärk, K., Berghold, C., Myllyniemi, A.L., Wasyl, D., Sunde, M. and Aarestrup, F.M., 2008. Prevalence of antimicrobial resistance among bacterial pathogens isolated from cattle in different European countries: 2002-2004, Acta veterinaria scandinavica, 50, 28.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jamali, H., Rezagholipour, M., Fallah, S., Dadrasnia, A., Chelliah, S., Velappan, R.D., Wei, K.S., and Ismail, S., 2014. Prevalence, characterization and antibiotic resistance of Pasteurella multocida isolated from bovine respiratory infection, veterinaria Journal, 202, 381–383.

    CAS  Google Scholar 

  • Katsuda, K., Kohmoto, M., Mikami, O., Uchida, I., 2009. Antimicrobial resistance and genetic characterization of fluoroquinolone-resistant Mannheimia haemolytica isolates from cattle with bovine pneumonia, Veterinary microbiology, 139, 74–79.

    Article  CAS  PubMed  Google Scholar 

  • Katsuda, K., Hoshinoo, K., Ueno, Y., Kohmoto, M., and Mikami, O., 2013. Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves. Veterinary microbiology, 167, 737–741.

    Article  CAS  PubMed  Google Scholar 

  • Kehrenberg, C., Schwarz, S., 2005. Plasmid-borne florfenicol resistance in Pasteurella multocida, Journal of antimicrobial chemotherapy, 55, 773–775.

    Article  CAS  PubMed  Google Scholar 

  • Kehrenberg, C., Schwarz, S., 2007. Mutations in 16S rRNA and ribosomal protein S5 associated with high-level spectinomycin resistance in Pasteurella multocida. Antimicrob. Journal of antimicrobial chemotherapy, 51, 2244–2246.

    Article  CAS  Google Scholar 

  • Kong, L.C., Gao, D., Gao, Y.H., Liu, S.M., and Ma, H.X., 2014. Fluoroquinolone resistance mechanism of clinical isolates and selected mutants of Pasteurella multocida from bovine respiratory disease in China, Journal of veterinary medical science, 76, 1655–1657.

    Article  CAS  PubMed  Google Scholar 

  • Magstadt, D.R., Schuler, A.M., Coetzee, J.F., Krull, A.C.1., O’Connor, A.M., Cooper, V.L., and Engelken, T.J., 2018. Treatment history and antimicrobial susceptibility results for Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolates from bovine respiratory disease cases submitted to the Iowa State University Veterinary Diagnostic Laboratory from 2013 to 2015, Journal of veterinary diagnostic investigation, 30, 99–104.

  • Michael, G.B., Kadlec, K., Sweeney, M.T., Brzuszkiewicz, E., Liesegang, H., Daniel, R., Murray, R.W., Watts, J.L. and Schwarz, S., 2012a. ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer, Journal of antimicrobial chemotherapy, 67, 91–100.

    Article  CAS  PubMed  Google Scholar 

  • Michael, G.B., Eidam, C., Kadlec, K., Meyer, K., Sweeney, M.T., Murray, R.W., Watts, J.L. and Schwarz, S., 2012b. Increased MICs of gamithromycin and tildipirosin in the presence of the genes erm (42) and msr (E)-mph (E) for bovine Pasteurella multocida and Mannheimia haemolytica, Journal of antimicrobial chemotherapy, 67, 1555–1557.

    Article  CAS  PubMed  Google Scholar 

  • Rímac, R., Luna, L., Hurtado, R., Rosadio, R. and Maturrano, L., 2017. Detection and genetic characterization of Pasteurella multocida from alpaca (vicugna pacos) pneumonia cases, Tropical Animal Health & Production, 49, 1325–1328.

    Article  Google Scholar 

  • Rose, S., Desmolaize, B., Jaju, P., Wilhelm, C., Warrass, R. and Douthwaite, S., 2012. Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida. Antimicrob Agents Chemother 56, 3664–3669.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sun, N., Liu, J.H., Yang, F., Lin, D.C., Li, G.H., Chen, Z.L. and Zeng, Z.L., 2012. Molecular characterization of the antimicrobial resistance of Riemerella anatipestifer, isolated from ducks, Veterinary microbiology, 158, 376–383.

    Article  CAS  PubMed  Google Scholar 

  • Sweeney, M.T., Papich, M.G., Watts, J.L., 2017. New interpretive criteria for danofloxacin antibacterial susceptibility testing against Mannheimia haemolytica and Pasteurella multocida associated with bovine respiratory disease, Journal of veterinary diagnostic investigation, 29, 224–227.

    Article  CAS  PubMed  Google Scholar 

  • Tang, X., Zhao, Z., Hu, J., Wu, B., Cai, X., He, Q. and Chen, H. 2009. Isolation, Antimicrobial Resistance, and Virulence Genes of Pasteurella multocida Strains from Swine in China, Journal of clinical microbiology, 47, 951–958.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tong, P., Sun, Y., Ji, X., Du, X., Guo, X., Liu, J., Zhu, L., Zhou, B., Zhou, W., Liu, G. and Feng, S., 2015. Characterization of antimicrobial resistance and extended-spectrum β-lactamase genes in Escherichia coli isolated from chickens, Foodborne Pathogens and Disease, 12, 345–52.

    Article  CAS  PubMed  Google Scholar 

  • Townsend, K.M., Frost, A.J., Lee, C.W., Papadimitriou, J.M. and Dawkins, H.J., 1998. Development of PCR assays for species-and type-specific identification of Pasteurella multocida isolates, Journal of clinical microbiology, 36, 1096–1100.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Van Engen, N.K., Stock, M.L., Engelken, T., Vann, R.C., Wulf, L.W., Karriker, L.A., Busby, W.D., Lakritz, J., Carpenter, A.J., Bradford, B.J., Hsu, W.H., Wang, C. and Coetzee, J.F., 2014. Impact of oral meloxicam on circulating physiological biomarkers of stress and inflammation in beef steers after long-distance transportation, Journal of animal science, 92, 498–510.

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Ying Zhang, Lei Liu, and Ren-ge Hu for their laboratory contributions.

Funding

This research was funded by the National Key Research and Development Plan (grant number: 2016YFD0501301), the National Natural Science Foundation of China (grant number: 31702293), the Science and Technology Development Plan of Jilin Province (grant number: 20170520074JH), Jilin Provincial Education Department “the 13th five-year” Science and Technology Project (grant number: JJKH20180696KJ), and the Jilin Agricultural University Research Foundation (grant number: 201702).

Author information

Author notes

  1. Xiu-Yun Jiang and Hong-Xia Ma contributed equally to this paper.

Authors and Affiliations

  1. Department of Basic Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Ling-Cong Kong, Zi Wang, Yi-Ming Wang, Wen-Long Dong, Bo-Yan Jia, Xiu-Yun Jiang & Hong-Xia Ma

  2. The Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Jilin, China

    Ling-Cong Kong & Hong-Xia Ma

  3. Liaoning Testing and Inspection Center for Quality & Safety of Veterinary Drugs, Feed and Livestock Products, Shenyang, China

    Duo Gao

Authors
  1. Ling-Cong Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zi Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi-Ming Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wen-Long Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bo-Yan Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Duo Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiu-Yun Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hong-Xia Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Xiu-Yun Jiang or Hong-Xia Ma.

Ethics declarations

Statement of animal rights

This study was carried out in accordance with the recommendations of ‘Laboratory animal-Guideline for ethical review of animal welfare (GB/T 35892-2018), General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China’. The protocol was approved by the Animal Care and Use Committee of the Jilin Agricultural University.

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kong, LC., Wang, Z., Wang, YM. et al. Antimicrobial susceptibility and molecular typing of Pasteurella multocida isolated from six provinces in China. Trop Anim Health Prod 51, 987–992 (2019). https://doi.org/10.1007/s11250-018-1754-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-018-1754-9

Keywords

Search

Navigation