Identifying Efficient Transport Pathways in Early‐Wood Timber: Insights from 3D X‐ray CT Imaging of Softwood in the Presence of Flow

Wider use of timber has the potential to greatly reduce the embodied carbon of construction. Improved chemical treatment could help overcome some of the barriers to wider application of timber, by furthering the durability and/or mechanical properties of this natural material. Improving timber treatment by treating the whole volume of a piece of timber, or tailored sections thereof, requires sound understanding and validated modelling of the natural paths for fluid flow through wood. In this study we carry out a robust analysis of three-dimensional X-ray CT measurements on kiln-dried softwood in the presence of flow and identify small portions of early-wood which are uniquely capable of transporting fluids—herein ‘efficient transport pathways’. We successfully model the effects of these pathways on the liquid uptake by timber by introducing a spatial variability in the amount of aspiration of the bordered pits following kiln drying. The model demonstrates that fluid advances along these efficient transport paths between 10 and 30 times faster than in the remainder of the timber. Identifying these efficient transport pathways offers scope to improve and extend the degree to which timber properties are enhanced at an industrial scale through processes to impregnate timber. * R. Pini r.pini@imperial.ac.uk 1 Department of Civil and Environmental Engineering, Imperial College London, London SW7 2AZ, UK 2 Department of Chemical Engineering, Imperial College London, London SW7 2AZ, UK 3 Present Address: UCL Mechanical Engineering, Research Complex at Harwell, RAL, Didcot, Oxfordshire OX11 0FA, UK 4 Institute for Infrastructure and Environment, School of Engineering, University of Edinburgh, Edinburgh EH9 3FG, UK 5 Melville Laboratory for Polymer Synthesis, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK 6 Department of Architecture, University of Cambridge, Cambridge CB2 1PX, UK 7 Department of Applied Mathematics and Theoretical Physics, Centre for Mathematical Sciences, University of Cambridge, Wilberforce Road, Cambridge CB3 0WA, UK H. C. Burridge et al. 1 3


Experimental
We conducted two experiments on a specimen of Sitka spruce (Picea sitchensis (Bong.) Carrière), as reported in Table 1 together with the conditions of the tests. Two spontaneous imbibition tests were also carried out on a separate sample of the same species, and these are described in Sect. 2.4.

Materials
A 200-mm-long specimen was machined into a cylindrical shape (50 mm in diameter) from flat-sawn, kiln-dried timber supplied by BSW Timber Ltd (UK). From a first visual inspection (see also Fig. 1), the specimen accommodates six growth rings, which are approximately parallel to one another ( ∼ 1 cm apart) and aligned with the longitudinal axis, z, which also corresponds to the direction of flow imposed in the experiments. These growth rings cover the full length of the specimen, but are distorted by the presence of a knot which crosses the entire specimen at a slight angle to the vertical direction, y. The specimen bulk density, estimated from mass/geometrical volume, takes a value b = 0.38 g/cm 3 . An average specimen porosity of = 72.4% is obtained by assuming an average specimen skeletal density of s = 1.37 g/cm 3 . The latter has been obtained by Wu et al. (2017) from helium pycnometry on consolidated sister specimens and is lower than the so-called dry Table 1 List of experiments carried out in this study. These have been conducted by injecting liquid (water, W, or ethyl acetate, EA) at constant pressure ( P inj ). During each experiment, the specimen was kept under a constant external pressure ( P c ) and the volume of injected liquid ( V inj ) was continuously recorded by the pump. The specimen bulk density ( b ) is estimated from mass/geometrical volume, and its porosity is obtained by assuming an average specimen skeletal density of s = 1.37 g/cm 3 cell wall density ( m ≈ 1.5 g/cm 3 ) due to the presence of inaccessible pores and/or closed lumens (see Wu et al. (2017) for a full discussion). Two liquids were used for the solvent injection experiments, namely de-ionized water and ethyl acetate (anhydrous, 99.8%, Sigma-Aldrich), as these solvents produce a different degree of tangential swelling of Sitka spruce, namely 8.4% (water) and 2.6% (ethyl acetate), respectively (Mantanis et al. 1994). The relevant physical properties of the two liquids are provided in Table 2.

Apparatus
The solvent injection experiments were performed using the experimental setup depicted in Fig. 2. A custom-made aluminium specimen holder has been used that can accommodate a cylindrical specimen of 5 cm in diameter and of variable length. The specimen is positioned between two aluminium end plates with embedded circular grooves for enhanced fluid distribution (see inset in the figure). Due to timber's fairly large permeability, two layers of qualitative filter paper (Whatman, Grade 1, 11 μm pore size) are additionally placed between the inlet face of the specimen and the aluminium end plate, so as to minimise boundary effects caused by the detail of the end cap. The whole arrangement is jacketed with two layers of polyolefin heat shrink tubing (RS Pro, UK) to achieve a tight seal around the specimen. To further minimise bypass flow during the experiments, the annulus between the jacketed specimen and the aluminium specimen holder is filled with water that is kept at a constant pressure P c using a syringe pump (ISCO Teledyne, Model 1000D). The specimen holder is placed horizontally on the bed of the scanning instrument (Universal Systems HD-350 X-ray CT scanner) and is connected to an injection pump (ISCO Teledyne, Model 1000D) and an effluent collector by

Experimental Procedure and X-ray Imaging
Water and ethyl acetate were used as injection fluids (in this chronological order), and both experiments have been carried out at room temperature and ambient pressure conditions. Prior to each experiment, the timber specimen was dried for 24 hours at 60 °C and subsequently stored in the laboratory in air (r.H. ≈ 30%). The similarity of the dry 3D density profiles obtained prior to the experiment with water and ethyl acetate (see Results section) indicates that the microstructure of the specimen has not been altered by the drying process. After assembling the aluminium specimen holder and placing it on the bed of the scanner, the annular confining pressure on the specimen was increased and set to a value of 0.8 MPa. A full X-ray scan was taken of the dry specimen, which was subsequently no longer moved. The injection lines were then purged with the solvent, connected to the inlet end cap and injection started. For the experiments reported here, the latter was achieved by maintaining a constant injection pressure of 0.4 MPa, while keeping the effluent line open to atmospheric pressure (i.e. a pressure drop, ΔP = 0.3 MPa). Stable injection pressure was achieved within 1 minute. Throughout the experiment, X-ray scans of the specimen were taken at regular time intervals for 2-mm-thick slices at a resolution of (250 × 250) μm 2 and an acquisition time of approximately 10 min for one full tomogram. Additional imaging parameters are: field of view, 120 mm; energy level of radiation, 120 eV; tube current, 200 mA. The X-ray tomograms reconstructed by the scanner are provided in terms of 2D maps of spatially distributed CT numbers in Hounsfield units, HU. For X-ray energies above 100 keV, i.e. where medical CT scanners normally operate, the CT number can safely be assumed to be linearly proportional to the bulk density of the scanned object, i.e. b = mCT + p , where m and p are constant parameters that can be obtained upon calibrating the instrument with appropriate phantoms . Ideally, the latter are chosen, so as to cover the expected range of dry and wet bulk density values, thus avoiding extrapolation. This linear relationship between density and Hounsfield numbers has been previously validated for wood samples representing a large range of densities ( 130 − 1300 kg ⋅ m −3 ) and for the current voltage applied in our study (Freyburger et al. 2009). The CT number for any given voxel i in the system can be expressed as the summation of the volumetric fractions of each component in the mixture (Vinegar and Wellington 1987), where CT s , CT l and CT air are the CT numbers of the pure components (solid material, liquid and air, respectively), while and S are the porosity and the liquid saturations, which may vary from voxel to voxel. In this study, because of the fairly low density of timber ( b ≈ 0.4 − 1.1 g cm −3 in the dry and fully water-wet state), air and water were used as calibration standards and the following parameters were obtained: m = 1.04 × 10 −3 g cm −3 HU −1 and p = 0.935 g cm −3 . Hence, direct estimation of bulk density values from the CT numbers is possible and, accordingly, of the mass of liquid uptake per unit (bulk) volume, i.e. (1) where the subscripts "dry" and "wet" refer to scans acquired prior to ( S = 0 ) and after start of liquid injection. Note that (2) is applied on the voxel scale, whereas slice-(or specimen-) averaged properties are calculated using slice-(or specimen-) averaged CT numbers. The corresponding porosity ( = 1 − b,dry ∕ s ) and liquid saturation values ( S = m l ∕ l ) can be estimated by further assuming in (1) that the CT number (and, therefore, the density) of the solid component does not vary spatially; this assumption is justified in view of the rather uniform chemical composition of timber in terms of its three major constituents (cellulose, hemicellulose and lignin) (Dinwoodie 1981).
3D reconstructions of the timber specimen are obtained using (0.5 × 0.5 × 2) mm 3 voxels, thereby enabling the estimation of the properties above at the voxel scale with a precision of b ± 4.5 × 10 −3 g cm −3 , m l ± 7 × 10 −3 g cm −3 , ± 0.35 % and S ±1%. Highresolution imaging was attained for a selected slice located 100 mm from the inlet face of the specimen by acquiring (and averaging) 15 repeated scans following the acquisition of the full tomogram; this enables achieving a precision of ±1×10 −3 g cm −3 on the estimated density values at the original scanner resolution of (250 × 250) μm 2 . Images reconstruction and data analysis were carried out using in-house MATLAB routines.

Spontaneous Imbibition Tests
For the spontaneous imbibition experiments, a new 50-mm-long, 200-mm-diameter cylindrical specimen of Sitka spruce was used. Prior to this experiment, the specimen was dried for 24 hours at 60 °C and subsequently stored in the laboratory in air (r.H. ≈ 30%). The specimen was wrapped in a FEP heat-shrink tube (Polyflon Technology Ltd.) and placed vertically in a plastic beaker. The heat-shrink tube was cut longer than the timber specimen, so as to leave a gap of about 5 mm between the bottom face of the wood specimen and the base of beaker. The whole arrangement was positioned on the bed of the scanning instrument. To initiate the imbibition experiment, water was carefully poured into the beaker to reach a level 15 mm above the bottom face of the timber specimen. The specimen was imaged at ambient conditions (20 °C) using the X-ray CT scanner. X-ray scans of a 1-mmthick central section of the specimen were taken prior to and during the imbibition process at regular time intervals at a resolution of (250 × 250) μm 2 (additional imaging parameters: field of view, 120 mm; energy level of radiation, 120 eV; tube current, 200 mA). The scan analyzed in this study was taken 70 hours after the start of the experiment.
An additional imbibition test was carried out on a smaller specimen to be imaged at a higher spatial resolution in a Zeiss Xradia 500 Versa 3D X-ray microscope. The small wood plug was drilled from the larger specimen to yield a 7-mm-long, 4-mm-diameter cylindrical specimen. The specimen was wrapped in a heat-shrink tube, and subsequently, its base attached to a specimen holder that fits to the rotation stage of the imaging instrument using a two-component glue. The glue was applied carefully just before its hardening point to avoid its penetration into the wood specimen. To initiate the imbibition experiment, the gap (of a few mm) created by the heat-shrink on the top end of the specimen was filled with water, which was doped with potassium iodide (25 wt%) to enhance image contrast. The specimen was imaged at ambient conditions (20 °C) with 80 kV and 7 W settings prior and during imbibition (approximately 5 min after start of the experiment). The images were binned during scanning to obtain a size of 1000 3 voxels. For each tomogram, 1000 projections were taken, with a resulting computed pixel dimension of 4 μm/pixel. The tomograms were reconstructed using proprietary software provided by Zeiss. The reconstructed tomograms were processed by using Avizo-9 software.

Three-Dimensional Imaging of Liquid Transport
X-ray CT scanning allows for precise three-dimensional imaging of the bulk density of large timber specimens at a resolution of 0.5 mm 3 . Figure 3 shows a reconstruction sliced through its centre both vertically and horizontally for the dry timber specimen. The denser wood constituting the knot and the late-wood growth rings are clearly visible features in this specimen, varying in density between approximately 0.2 g ⋅ cm −3 and 0.6 g ⋅ cm −3 .
X-ray CT scans of the same specimens were also obtained in the presence of liquid flow, enabling the three-dimensional mapping of the liquid saturation in the timber specimen upon combination with data from the dry scan. Figure 4 shows the same timber specimen, with the top left-hand image presenting the reconstruction in terms of local (voxel) porosity. The remaining five images in the figure show these saturation maps at various time intervals throughout the experiment (in this case lasting over 22 hours) injecting water. As one would expect, the highest levels of saturation are evident at the injection end. However, we observe that in all images the locations with high levels of liquid saturation closely follow the pattern of the late-wood growth rings. It is evident (for example the image labelled 183 min) that water travelling along these paths reaches the far end much quicker than water being injected along other paths. Indeed it was observed that water broke through the end of the specimen at these certain locations as early as 150 minutes into the experiment.
This pattern of pathways of relatively high saturation, resembling the form of the latewood growth rings, was even more quickly evident when the specimen was injected with ethyl acetate, a solvent which causes less swelling in wood than water (Mantanis et al. 1994) ( Figure 5). Again, the regions of high saturation also portray the outline of the knot within the specimen, highlighting that cells around the knot saturate more easily and enable relatively efficient liquid transport around and past the knot.
For a qualitative examination of the liquid uptake and saturation we selected data from the location z = 15 mm. A location near the inlet was chosen so that in both experiments The map on the top left corner represents the porosity, , of the specimen (acquired prior to starting injection), while the remaining maps represent liquid saturations, S, and refer to different times throughout the experiment. Injection is carried out at a constant inlet and outlet pressures of 0.4 and 0.1 MPa, respectively. One full tomogram is acquired in about 10 min, and the time shown on top of the map represents the mean between first and last scan. Voxel dimensions are (0.5 × 0.5 × 2)mm 3 , and direction of flow is from left to right similar, with the sixth (right-hand images) showing the high saturation levels reached in this plane for the (longer running) water experiment. The first five saturation maps highlight similar patterns in saturation and further show that ethyl acetate more quickly saturates the specimen, indicating faster uptake.

Localisation of Efficient Transport Pathways
Quantitative differences in the uptake are better evidenced by examining the central plane ( z = 100 mm) of the specimen.   readily obtained by subtracting the two sets of data (wet and dry profiles) and are shown in Fig. 7c (water) and Fig. 7f (ethyl acetate), respectively. The faster uptake of ethyl acetate is clearly evidenced by higher mass contents being attained at 375 min than were attained for water at 1350 min. Within the six plots of Fig. 7, dashed horizontal lines mark the location of the dry density peaks and aid careful observation of the relative position of the peaks in liquid mass content. It can be observed that for both water and ethyl acetate experiments the two sets of peaks are not precisely aligned-the peaks in liquid mass content occur at locations systematically below the peaks in dry bulk density. This indicates that while the saturation patterns in Figs. 4, 5 and 6 mimic the pattern of the late-wood growth rings, the patterns are not co-located and are, in fact, offset slightly from one another. Note that the liquid transport along these paths is relatively fast as the offset is already apparent at early times (6-7 min after injection).
To better evidence this offset between peaks in density and liquid mass content, data were collated from ∼ 100 vertical transects at the plane z = 100 mm and the origin of a local coordinate was established at each density maximum within a single transect (corresponding to a late-wood growth ring). The resulting 459 (dry) bulk density profiles in these local coordinates are plotted in the lower two panes of Fig. 8. The curvature of the growth rings (for example, see the inset 2D density maps) highlights that the growth of the tree occurred from the top to the bottom of the specimen in our particular alignment. To the left of the origin (density maximum) the spatial gradient in density is more gradual, while to the right the density drops rapidly (corresponding to the tree growth ceasing over winter and then restarting, laying down much lower-density wood, with the onset of the growing season). The strong similarity between the two independent sets of dry data indicates that the drying procedure prior to each experiment did not alter the wood's microstructure. The upper two panes show the corresponding liquid mass content measured in our local coordinate system (the left-hand pane shows the data for water and the right-hand ethyl acetate).
In both of the lower and upper panes of Fig. 8 the regions of relatively high-density latewood (with the threshold set to 0.43 g/cm 3 , see Reynolds et al. (2018)) are shown by darker colours. What is clear from the upper two panes is that these high-density dark regions do not perfectly align with the peaks in liquid mass content for either fluid. More specifically, these peak liquid mass contents contribute about 45% (for ethyl acetate) and 31% (for water) of the total liquid content. In fact, it is evident that the peaks in density and the peaks in liquid mass content are offset by approximately +0.7 mm ( ±0.25 mm), indicating that these efficient paths of liquid transport are evident both for the transport of water and for ethyl acetate. We note that the observed offset is not created by image resolution, because the two sets of scans (dry vs. water as well as dry vs. ethyl acetate) have been reconstructed at the same image resolution. Notably, these paths lie just on the new growth side of the peak densities, i.e. they lie just outside the late-wood growth rings. Such a finding is novel and contrary to the assumptions made in other studies that the preferential pathways lie within the late-wood cells (Sedighi-Gilani et al. 2012;Javed et al. 2015;Desmarais et al. 2016). The conclusion that preferential transport occurs in the smaller late-wood cells led previous researchers (Desmarais et al. 2016) to logically surmise that capillary effects must be dominant and therefore that capillary effects give rise to the fastest transport. However, we showed that, even when driving pressures of just one atmosphere were applied, capillary effects accounted for less than 10% of the liquid transport (Burridge et al. 2019). Our findings resolve this apparent contradiction. By identifying that the efficient transport pathways occur away from local peaks in density we can conclude that capillary effects do not give rise to relatively fast transport under these modest driving pressures. Indeed from our CT measurements and the work of Reynolds et al. (2018) we can imply that the pore space within the efficient transport paths are of intermediate size-see the data in Figs. 7 and 8, which highlights that the peaks in the transport occur at locations where the density is low, i.e. within some of the largest early-wood cells. Therefore, the structure and number of bordered pits within the efficient transport paths must vary to some extent-we exploit this knowledge in our modelling (Sect. 4.1). It is worth noting that such a finding warrants reexamination of industrial approaches to the liquid treatment of timber. These may also encompass the energy-intensive timber drying process, particularly those practices that exploit chemical treatment to minimise dimensional changes in wood and the associated damage (Kumar 2007). As we have studied kiln-dried timbers, which have modified microstructures (e.g. aspirated pits) in comparison with greenwood, our work could also offer insights into the reversibility of the drying process.   Fig. 8 Density variations in timber and associated distribution of liquid content following injection. The bottom plots represent bulk density profiles for each growth ring and for ∼ 100 vertical transects (459 profiles in total) within the slice of the timber specimen located 100 mm from the injection port (shown in the inset of the plot, voxel dimensions: (250 × 250) μm 2 ). For each profile, location "zero" is set as the point where the largest bulk density is measured and the separation between late (grey symbols) and earlywood (yellow symbols) is arbitrarily set at the mid-point between minimum and maximum densities, b = 0.43 g/cm 3 . The plot for water injection is on the left and for ethyl acetate is on the right. On the top plots, the same profiles are shown, but in terms of liquid content measured at 260 min (water) and 275 min (ethyl acetate) after start of injection. The darker and lighter symbols denote locations identified in the bulk density profiles as late-and early-wood, respectively. Note that the late-wood density peak and the peak saturation levels are not aligned

Discussion
Timber is the only widely used construction material we can grow , and architects and engineers are ever more demanding of its use (Cornwall 2016). Extending the use of wood in construction offers significant scope for mitigating the effect of CO 2 emissions on our climate (Tollefson 2017). Timber use can be increased by better modifying its mechanical properties or improving its resilience with liquids. At a laboratory scale, previous work that involves exposing the timber microstructure to aqueous chemical solutions which delignify the specimens prior to compression, demonstrates that timber can be processed into materials with unprecedented mechanical properties (Song et al. 2018;Frey et al. 2018). In order to industrialise this process and exploit further natural benefits of timber, accurately predicting the transport of these liquids within timber is crucial. Here, we have examined the liquid transport in kiln-dried, moderately sized ( l = 200 mm) timber specimens using a medical-grade CT scanner and identify previously unreported efficient transport paths within the timber. Identifying these pathways provides a step change in our understanding of the transport of liquids within timber, which we expect to lead to significant improvements in industrial treatment processes.
We further discuss our findings below by presenting a model to describe liquid transport through the timber specimen by using a geometrical parameterisation of the pore space informed by high-resolution imaging. Furthermore, we reconcile our findings with those of previous radiographic studies of timber (Sedighi-Gilani et al. 2012;Javed et al. 2015;Desmarais et al. 2016) by discussing results of experiments of spontaneous imbibition using both medical-grade CT scans and a high-precision micro-CT scanner.

Prediction of Liquid Transport in Timber
Following (Burridge et al. 2019), we utilise a model based on the Lucas-Washburn equation (Lucas 1918;Washburn 1921) modified to reflect the statistical distribution of the timber pore space. We update the model (Burridge et al. 2019) to reflect the experiments presented herein; namely, that the flow was forced, by imposing a constant driving pressure, to enter the timber specimens from one end and was free to leave (at atmospheric pressure) from the other. In so doing, the model predicts both the liquid uptake by the timber and the flow rate through the specimen-these predictions can be directly compared to estimates made from our experimental CT measurements and the injection pump logs, respectively. Moreover, whereas (Burridge et al. 2019) looked to model pore space within early-and late-wood phase of wood (and the statistical variation within each), we herein look to examine the effect of efficient transport paths within the timber. As such, the spatially resolved time-lapse data presented in this study not only uncover details of the displacement process, but also provide a unique opportunity to inform the parameterisation of the model itself.
We parameterise the model using standard values for the fluid properties (see Mantanis and Young (1997); Speight (2005) and Table 2). In our experiments we utilise timber specimens taken from the same batch of Sitka spruce as those examined by Reynolds et al. (2018). We base our geometrical parameterisation of the pore space on the values reported from their CT and micro-CT measurements (Reynolds et al. 2018). Namely, we take the values: L T = 1.25 ± 0.25 mm for the tracheid lengths (where the tolerances reflect the standard deviation); r T = 16 ± 3 m for the tracheid radii; and = 0.73 for the porosity.
The only remaining parameters, not recorded by their micro-CT measurements, remain the number of bordered pits per tracheid for which we take the values reported by Burridge et al. (2019) and the characteristic diameter thereof, 2a p -which remains the only fitting parameter herein. From the data shown in Fig. 8 we estimate that the efficient transport paths account for about 7% of the timber volume and we therefore take = 0.93 in the model. Figure 9 presents the experimental data from our CT measurements and the injection pump logs and compares these to model predictions. In order to fit the data, we take 2a p = 0.078 m for the characteristic diameter of the bordered pits within the bulk of the specimen, for those within the efficient transport paths we take 2a p = 0.14 m for the experiments using water and 2a p = 0.31 m for the ethyl acetate experiments-all of which lie well within the range of measured values reported by Siau (1984). Figure 9a and b shows good agreement between the model predictions and the experimental data over a range of timescales from minutes right up to a day in the experiment using water. The model quantitatively captures the decreasing flow rate as the experiments evolve and correctly predicts the time at which liquid breaks through the specimen (reflected by the separation of the dashed and solid line for the model predictions, and the separation of the circular and triangular symbols for the experimental data). Furthermore, as the liquid break through signifies the time at which the efficient transport paths are (predominantly) saturated, thereafter the model predicts that the rate of uptake decreases dramatically (by a  Fig. 9 Liquid injection and liquid uptake history for the experiments with water (blue) and ethyl acetate (red). In all plots, lines mark predictions from the model ( §4.1), symbols mark experimental data; the latter include two sets of independent measurements, namely injection pump logs (filled circles) and X-ray CT (hollow triangles). a Injection and uptake rate for water; b injection and uptake rate for ethyl acetate; c cumulative injection and uptake; d liquid uptake shown in terms of saturation factor of approximately 3 for the water experiments and 10 for the ethyl acetate)-this dramatic decrease in uptake is precisely mirrored by the experimental data. Crucial to application, the total injected volume (Figure 9c) is predicted typically to an accuracy of within 20%, and the liquid saturation (predictions of which would be vital to improve the industrial treatment of timber) is always accurately predicted to within 10% (Figure 9d). Moreover, the model enables us to examine the rate at which fluid advances along the efficient transport paths. We find that, on average, liquid advances along the efficient transport paths at rates between 10 and 30 times faster than in the bulk of the specimen. This highlights the significant role that these pathways must play in the transport within industrial sized pieces of timber. Two comments are worth making with respect to the above analysis. First, our experiments used two fluids which are known to interact very differently with the cellular structure of timber (Section 2.1 and Table 2). It was necessary to characterise the bordered pits in the model differently for the two fluids, and this may indicate some effect of swelling on the flow passing through the pits-this remains a challenge for future research to investigate. Second, kiln drying causes aspiration of the bordered pits in most softwoods and its extent varies depending on the type and harshness of drying (Phillips 1933). In our approach to the modelling, we have considered distinct values of the diameter of the bordered pits in the bulk of the specimen and in the efficient transport pathways. It follows that the flow behaviour observed in this study for both water and ethyl acetate may be the result of a characteristic spatial variability in the amount of aspiration following kiln drying. Future experiments where the same experimental protocol is applied to samples that have never been kiln-dried will provide more insight into this phenomenon.

Flow Driven by Spontaneous Imbibition
Due to restrictions on the specimen size and the scan times we were unable to make reliable observations of the pressure-driven transient flow via micro-CT. Thus, in order to validate our findings and compare to those of existing studies we carried out two additional sets of experiments in which the flow was driven solely by capillary action, herein spontaneous imbibition. These enabled measurements taken with a) a micro-CT scanner (resolution (4 × 4 × 4) μm 2 on specimens of length 7 mm) to be compared to those taken with b) our medical grade CT scanner (resolution (0.25 × 0.25 × 1)mm 3 on specimens of length 200 mm). The results of these experiments are shown in Fig. 10 and Fig. 11, respectively, enabling the visualisation and quantification of water uptake at different depths within the specimen (corresponding to different levels of liquid saturation). The results are illuminating, showing that, in agreement with previous studies of spontaneous imbibition (Sedighi-Gilani et al. 2012;Javed et al. 2015;Desmarais et al. 2016), the dominant transport did occur in the smallest late-wood cells-as would be expected under capillary action. In fact, the 2D tomograms shown in Fig. 10 provide direct evidence of significant accumulation of water just inside the late-wood growth rings; similarly, the profiles depicted in Fig. 11 indicate that-when liquid uptake is significant (bottom plot)-the local maxima in the bulk density align by and large with those in the dry state. However, careful examination of the data of our CT scans, micro-CT data and the images presented in other studies, e.g. Sedighi-Gilani et al. (2012), shows that there is also significant transport in certain of the largest early-wood cells-contrary to existing expectations, and, moreover, in agreement with the observations from our independent pressure-driven flow experiments. We note that, while the terminal rise of the water will (for these experiments) be greatest in the smallest cells, identifying efficient transport paths in early-wood cells (for which the viscous retardation is least) provides sound reasoning as to why significant volumes of water rise more quickly through some of the largest early wood cells.

Conclusions and Implications
We have exploited medical grade CT imaging techniques with high-precision injection equipment to examine a widely used kiln-dried softwood timber in the presence of pressure-driven flow. Knowledge of the timber micro-structure informs our analysis and enables the identification of efficient transport pathways within early-wood cells. We  Fig. 10 2D tomograms acquired during a spontaneous imbibition experiment with water on the small 4-mm-diameter, 7-mm-long timber specimen. The first three tomograms on the left (voxel dimensions: (4 × 4) μm 2 ) refer to different depths within the specimen, from left (nearest the water source (0 mm, 0.6 mm and 1.2 mm), with the origin of the vertical coordinate set arbitrarily to the height of the first image). Water (highlighted in green) can be seen to have travelled farthest in the late-wood cells; crucially, significant transport can also be observed in a band of early-wood cells ∼1 mm from the early-to late-wood transition. The last two tomograms on the right provide a comparison with images obtained on the medical CT scanner (voxel dimensions: (250×250) μm 2 ) on the large specimen (images are not registered)  Fig. 11 2D vertical cross section of the large timber specimen acquired prior to (image on the left) and during spontaneous water imbibition (image on the right, 70 hours after exposure to the water). The three plots on the right-hand side of the figure are bulk density profiles at three different depths (filled symbols refer to the dry specimen, while empty symbols refer to the wet specimen) successfully model the flow within these pathways and within the bulk of the specimens and demonstrate that liquids are transported between 10 and 30 times faster along these efficient transport paths than within the bulk of the specimen. We validated these findings and successfully resolved the apparent inconsistency within the literature regarding the role of capillary effects and liquid transport in late-wood cells, by carrying out experiments of spontaneous imbibition, taking measurements using both our medical CT scanner and a micro-CT scanner.
The findings from our study are largely based on observations made on two specimens; this is undoubtedly a small number for a variable material, such as wood. Yet, a key aspect of our approach is the analysis of spatially resolved time-lapse fluid saturation data within the sample under study. To identify the efficient transport pathways, we have evaluated approx. 100 vertical transects across the wood specimen (resulting in approx. 500 density profiles and 20,000 saturation points) and we have done that for two independent experiments (water injection and ethyl acetate injection)-see Fig. 8. We believe that this approach provides for a solid statistical basis in the evaluation of the fluid displacement process. In the approach to modelling, we have combined these data with a density-microstructure relationship derived for the same species of timber (Reynolds et al. 2018) and hypothesised that the first early-wood pits resist aspiration more strongly than the pits in the bulk of the specimen. That we are able to predict the macroscopic uptake and injection time histories based on these physical measurements demonstrates that we have captured the specific physics of this process in our kiln-dried specimens.
Combining knowledge of these pathways with our predictive model we expect to be able to significantly improve the industrial treatment of timber, thereby enabling its mechanical properties and/or its resilience to be more effectively modified and better tailored to specific needs. Our findings therefore offer scope to extend the use of timber in construction-the only sustainable construction material that can be used at scale )-and so aid the positive changes that would be provided to our atmosphere and climate (Tollefson 2017). We propose that these techniques should be applied to hardwood timbers, green-wood timber, and be carried out also at the scale of micro-CT measurements. In so doing our findings offer genuine scope to increase the pallet of timber products available to architects and engineers and to better understand the energy-intensive timber drying process. are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/.