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Conversion of squid pens to chitosanases and dye adsorbents via Bacillus cereus

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Abstract

The purpose of this study was to reclaim squid pens for the production of chitosanases and biosorbents of food colorants and industrial dyes via Bacillus cereus fermentation. B. cereus TKU044 produced chitosanases at a high productivity of 2.66 U/mL, when squid pens were used as the sole carbon and nitrogen source. An extracellular chitosanase, with a molecular weight of 43 kDa, was purified 286-fold with a 16% activity yield. TKU044 and five other chitosanase-producing strains of B. cereus have been used as biosorbents to investigate their adsorption capacity on 0.025% (w/v) of colorants and dyes. For colorants, only Red 7 was adsorbed by the five B. cereus cells, whereas all tested B. cereus strains showed adsorption effects with the dyes. The adsorption of Yellow 4 by squid pens before and after 1 day of fermentation by B. cereus was also studied.

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Acknowledgements

This work was supported in part by a grant from the Ministry of Science and Technology, Taiwan (MOST 105-2313-B-032-001).

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Authors and Affiliations

  1. Department of Chemistry, Tamkang University, New Taipei City, 25137, Taiwan

    San-Lang Wang, Hao-Ting Yu, Min-Hsiung Tsai, Chien Thang Doan & Van Bon Nguyen

  2. Life Science Development Center, Tamkang University, New Taipei City, 25137, Taiwan

    San-Lang Wang

  3. Department of Experimental Biology, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Chien Thang Doan & Van Bon Nguyen

  4. Division of Agro-Forestry System, Western Highlands Agriculture and Forestry Scientific Institute, Buon Ma Thuot, 630000, Vietnam

    Van Chung Do

  5. Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Anh Dzung Nguyen

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  1. San-Lang Wang
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  2. Hao-Ting Yu
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  4. Chien Thang Doan
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  5. Van Bon Nguyen
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Correspondence to San-Lang Wang or Anh Dzung Nguyen.

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Wang, SL., Yu, HT., Tsai, MH. et al. Conversion of squid pens to chitosanases and dye adsorbents via Bacillus cereus. Res Chem Intermed 44, 4903–4911 (2018). https://doi.org/10.1007/s11164-018-3343-y

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  • DOI: https://doi.org/10.1007/s11164-018-3343-y

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