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Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to enhance metabolite production in adventitious roots of Pseudostellaria heterophylla

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Abstract

Adventitious roots of Pseudostellaria heterophylla were cultivated in 3-L balloon-type bubble bioreactors (BTBB). The optimum angle of the base of the bioreactor was 90°. The rate of growth (64.96) and electrical conductivity (2.3 μS cm−1) were highest in the culture with the 90° bioreactor angle, and total saponins (9.61 mg g−1), polysaccharides (128.29 mg g−1) and amino acids (0.25 mg g−1) content were also maximum. We also investigated the effects of a single elicitor (methyl jasmonate; MJ) on accumulation of metabolites in P. heterophylla adventitious root culture in 3-L BTBB. The highest rate of growth (21.4) was achieved in the control group, in which the highest amino acids yield was 1.39 mg L−1. The highest total saponins (20.98 mg L−1) and polysaccharides (1.18 mg L−1) yields were obtained after addition of 10 mg L−1 MJ, and were, respectively, 1.28 and 1.23-fold higher than in the control group.

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Abbreviations

MS:

Murashige and Skoog (1962)

IBA:

Indole-3-butyric acid

MJ:

Methyl jasmonate

BTBB:

Balloon-type bubble bioreactor

EC:

Electrical conductivity

DW:

Dry weight

FW:

Fresh weight

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Acknowledgments

This research was funded by Tianjin University innovation fund, China (2013XQ0046), Ministry of Education New Teacher Fund, China (20130032120084).

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China

    Qin Wang, Hongyan Chai, Jing Li & Shuli Man

  2. Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China

    Juan Wang & Wenyuan Gao

Authors
  1. Qin Wang
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  2. Juan Wang
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  3. Hongyan Chai
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  4. Jing Li
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  5. Shuli Man
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Correspondence to Juan Wang or Wenyuan Gao.

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Wang, Q., Wang, J., Chai, H. et al. Optimization of balloon-type bubble bioreactor angle and methyl jasmonate concentration to enhance metabolite production in adventitious roots of Pseudostellaria heterophylla . Res Chem Intermed 41, 5555–5563 (2015). https://doi.org/10.1007/s11164-014-1681-y

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