Comparison of Metabolic Profiles of Fruits of Arctium lappa, Arctium minus, and Arctium tomentosum

Metabolites of the edible and medicinal plant Arctium have been shown to possess beneficial activities. The phytochemical profile of Arctium lappa is well-explored and its fruits are known to contain mainly lignans, fatty acids, and sterols. But the fruits of other Arctium species have not been thoroughly investigated. Therefore, this study compares the metabolic profiles of the fruits of A. lappa, Arctium tomentosum, and Arctium minus. Targeted metabolomics led to the putative identification of 53 metabolites in the fruit extracts, the majority of these being lignans and fatty acids. Quantification of the major lignans showed that the year of collection had a significant effect on the lignan content. Furthermore, A. lappa fruits contained lesser amounts of arctigenin but greater amounts of arctigenin glycoside than A. minus fruits. Regarding the profile of fatty acids, A. minus fruits differed from the others in the presence of linolelaidic acid. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s11130-024-01175-w.


Introduction
Arctium species, commonly known as burdocks, are widely distributed all over the world.However, only four species grow in the European continent, namely Arctium lappa L., Arctium minus (Hill) Bernh., Arctium tomentosum Mill., and Arctium nemorosum Lej.Burdocks are biennial herbs growing up to 2.5 m that can be distinguished especially by leaf petioles, by the type of inflorescences, and by the presence or absence of cobweb at typical hooked involucres. A. lappa (greeater burdock) has cordiform leaves with solid petioles, corymbiform inflorescences, and glabrous to loosely cobwebby phyllaries. A. minus (common burdock) is typical with its ovate leaves with hollow petioles, racemiform inflorescences, and young phyllaries are densely cobwebby, later on glabrous.As its name suggests, for A. tomentosum (woolly burdock) are typical densely cobwebby phyllaries.Inflorescences are formed in a corymb and leaf petioles are solid, sometimes hollow.A. nemorosum has hollow petioles, racemiform inflorescences, and loosely cobwebby phyllaries.During flowering or bearing fruits, its branches are typically bent down in an arc [1,2].
The edible roots and young leaves of A. lappa are frequently consumed especially in Asian countries as the main ingredients in several traditional dishes.Moreover, roots, leaves, and fruits of A. lappa play an integral part in traditional Chinese medicine.Extracts of different organs of Arctium species or compounds isolated from them are used especially in treating skin problems and possess significant antidiabetic, antioxidant, anti-inflammatory, gastroprotective, antimicrobial, and anticancer effects, as well as many other activities [3].In particular, fruits of A. lappa have been extensively studied in connection with their antidiabetic activity and could therefore serve as a functional food.
More than 300 metabolites of Arctium species have been identified so far (reviewed in [3]).Most of these metabolites are lignans, presented primarily in the fruits and seeds of A. lappa and A. tomentosum.Another large group of metabolites is represented by specific quinic acid derivatives found almost exclusively in the roots of Arctium species [4], but these have also been reported in aerial parts of A. lappa [5] and A. tomentosum [6].In addition, terpenoids and flavonoids can be found especially in burdock leaves, while fatty acids and sterols have been detected in all organs of A. lappa and A. tomentosum except the leaves.On the other hand, acetylenic compounds and saccharides are present in Arctium species almost exclusively in the roots [3,6,7].Most of the volatile metabolites have been identified primarily in the roots of A. lappa [3,8].
A. lappa is the most studied Arctium species, especially because its potential antidiabetic activity has been demonstrated both in vitro and in vivo [9], but only limited information is available for other Arctium species.The total phenolic content has been found to be lower in both the aerial parts and the roots of A. tomentosum than in those of A. lappa [6].However, the highest total phenolic content was detected in extracts of seeds of A. lappa [10].Several metabolites appear to be present in only some Arctium species, e.g., hyperoside has been found only in A. tomentosum [3,6,10].The concentrations of several compounds were found to differ significantly between different organs of A. lappa.The highest amounts of the triterpenoids betulinic acid, oleanolic acid, and lupeol, were found in the roots of plants collected in the vegetative stage [11].The content of bioactive compounds in a burdock depends not only on its species and the part of the plant selected, it is also influenced by environmental factors such as the temperature and precipitation the plant experiences [12].
Although the phytochemical profile of A. lappa has been well-investigated, other main burdocks that are widely distributed throughout Europe have, for some reason, been overlooked, and information about their content compounds is sketchy.A comparative study of the secondary metabolites found in different organs of selected Arctium species is, therefore, surely warranted.Presumably, Arctium fruits contain bioactive compounds and different species contain different contents.Therefore, this study compares the metabolic profiles of fruits of three main species growing in Europe (A.lappa, A. tomentosum, and A. minus) collected in the Czech Republic over two years.Targeted metabolomics based on burdock metabolites reported in the literature was used for this comparison.Furthermore, the two largest groups of metabolites found in the fruits were investigated in great detail: the major lignans in the fruits of these three burdock species were quantified and the profiles of fatty acids were compared.

Materials and Methods
The Materials and Methods section is presented in Online Resource 1.

Results and Discussion
Arctium species fruits were collected in 2021 and 2022, air-dried, and extracted.The amounts of extracts obtained per gram of the dried fruits differed significantly between the years of collection with less extract obtained in 2022.Differences in the composition of the fruit extracts might have been caused by environmental factors such as temperature, precipitation, shading, soil composition and type, soil microbiome, nitrogen content, and others [12].At the time the samples were collected, the mean temperature in the locality was 15.3 °C in 2021 and 13.3 °C in 2022.The difference in total precipitation was much greater with only 20.2 mm in 2021 and 59.8 mm in 2022.The level of precipitation was far below normal for the time of year in 2021 [13].The limited availability of water may have had an effect on the composition of the Arctium fruits, and the impact of this factor and also other factors with possible influence should be examined in detail in future experiments.

Metabolic Profiles
Following the removal of oily compounds by two extractions into n-hexane, targeted-metabolomic analysis using a database of the metabolites previously found in Arctium species [3] led to the putative identification of 53 metabolites in burdock fruit samples (Online Resource 2).23 of these metabolites were lignans, 10 fatty acids, 5 terpenoids, 6 were saccharides, 3 belong to the quinic acid derivatives, 4 were flavonoids, 1 was a sterol, and 1 an amino acid.This result shows that for free fatty acids the extraction into n-hexane was not effective.This is in accordance with Bazina & He who state that at neutral pH, more than 50% of certain free fatty acids are transferred from n-hexane into acetonitrile [14].From the putatively identified metabolites, 10 were identified using MSMS spectra or standards.
Qualitative analysis (Online Resource 2) showed that quinic acid derivatives and most flavonoids were absent in the 2022 samples.The 2022 samples also contained fewer representatives of saccharides, lignans and terpenoids.The metabolic profiles of the three species collected in the two years were visualized for comparison using PCA.Component 1, which accounted for almost 73% of the variability between the samples, separated the two years of collection (Fig. 1A).The compounds with the highest values of coefficients involved in component 1 are mostly lignans.On the other hand, the compounds with lower coefficients are fatty acids.The increase in the concentrations of flavonoids, lignans, and terpenoids could be attributed to stress caused by a lack of water in 2021, such as a droughtinduced increase in carbon-based secondary metabolites that has been reported in the aerial parts of plants [15].Lesser available water might also impact the lipid content of the fruits.A similar effect has been observed in castor beans, where water availability correlated linearly with the oil content [16].
All the species studied have a very similar profile, which is to be expected as plants from the same genus usually have similar contents.However, the A. lappa samples contained fewer terpenoid representatives than A. minus and A. tomentosum.Although the PCA analysis did not fully distinguish the Arctium species, A. minus showed greater values of components 2 and 4 than A. lappa (Fig. 1B).The compounds putatively identified as daucosterol and methyl linolenate had coefficients greater than 0.1 for components 2 and 4 and are therefore probably more abundant in A. minus fruits than in those of A. lappa.On the other hand, the compound putatively identified as arctigenin glucoside had coefficients lower than −0.1 for components 2 and 4 and is therefore probably more abundant in A. lappa fruits than in those of A. minus.

Quantification of Lignans
The major peaks in a typical base peak chromatogram of A. minus fruit extract were examined and those containing primarily lignan ions were identified as arctiin, arctigenin, diarctigenin, lappaol A, lappaol H, matairesinol, and matairesinoside (Fig. 2).Furthermore, the substance putatively identified as arctignan A/lappaol C/E/isolappaol C was isolated using semi-preparative HPLC and identified as lappaol C.
Arctiin was quantified using an LC-DAD method with its concentration expressed in milligrams per gram of the dry weight (DW) of samples of fruits obtained from A. lappa, A. minus, and A. tomentosum that were collected in two different years.The effects of species and year on the concentration of arctiin were assessed (Fig. 3A).The year of collection, the species, and the combination of the year of collection plus the species were found to have statistically significant effects on the concentration of arctiin.The mean concentration of arctiin combined across the three species was 36 ± 15 mg/g DW for 2021 and only 9 ± 4 mg/g DW for 2022 (P value lower than 0.001), with this decrease from 2021 to 2022 greater for A. lappa than for other two Arctium species.When the collection from the two years were combined, a statistically significant difference in the mean concentration of arctiin was found between A. lappa with 29 ± 24 mg/g DW and A. minus with 16 ± 11 mg/g DW (P value 0.038).
An LC-Q-TOF quantification method was validated for lappaol C, lappaol H, arctigenin, and matairesinoside (Online Resource 3).These lignans were quantified in micrograms per gram of the dried fruits of A. lappa, A. minus, and A. tomentosum collected in two different years, and the effects of the species and the year of collection on the concentrations of the lignans were assessed (Fig. 3B, C; Online Resource 4A).The year of collection was found to have a statistically significant effect of on the  A statistically significant effect of the species on the concentration of arctigenin was also found with a mean arctigenin concentration of 310 ± 120 μg/g of DW for A. lappa and a much greater mean arctigenin concentration of 960 ± 480 μg/g of DW (P value 0.008) for A. minus.The effect of combining the year of collection with the species on the concentration of the lignans was not statistically significant.
The greater concentrations of lignans found in the samples collected in 2021 by PCA, were confirmed for arctiin and lappaol C, and also for lappaol H and matairesinoside when they were related to the dry weight of the fruits.Statistically significant differences in the concentrations of arctiin and arctigenin in the species A. lappa and A. minus were also found.For the two collection years, A. lappa fruits contained more of the arctigenin glycoside named arctiin and less aglycone arctigenin than those of A. minus.This is in accordance with the PCA analysis that indicated a greater concentration of arctigenin glucoside in A. lappa than in A. minus.These results show clearly that the fruits of Arctium species are very rich sources of arctiin.Although the concentration of arctiin seems to be too high, our results are otherwise in good agreement with previous reports [17,18].Furthermore, lappaol C and lappaol H were other most abundant compounds which is consistent with the results reported for fruits of A. lappa [19].Among lignans of fruits of A. tomentosum, only arctiin, arctigenin, and lappaols A and F were previously identified [3].Comprehensive studies dealing with quantification of lignans in fruits of A. minus and A. tomentosum are missing.
only the extracts of A. minus contained the trans-fatty acid linolelaidic acid, a metabolite with potentially adverse effects on human health, including an increased risk of coronary heart disease and the promotion of inflammation [20,21].
A. lappa fruits seemed to have a more favorable composition of fatty acids with a lower content of oleic acid than those of A. tomentosum and a greater content of polyunsaturated linolenic acid than those of A. minus.However, the difference in the linolenic acid content may have been caused by incomplete extraction of this acid from acetonitrile into n-hexane, as in the acetonitrile fraction subjected to metabolomic analysis, the amount of linolenate was greater in A. minus extracts than those of A. lappa, which is in contrast to the results obtained by the analysis of fatty acids.Comparison with the literature is problematic as reports dealing with the fatty acid composition of Arctium species fruits are scarce.Previously, the seeds of A. lappa and A. minus have been found to be rich in linoleic acid (C18:2n6c) [22,23], which is in good agreement with our results.Interestingly, although linolelaidic acid has not been observed in A. minus seeds previously, Morris et al. did detect the presence of trans-3,cis-9,cis-12-octadecatrienoic acid [23].Therefore, A. minus is potentially a source of harmful trans fatty acids.Unfortunately, no report of the fatty acid composition of A. tomentosum fruits has been found.Only one study describes the fatty acid composition of the inflorescences of A. tomentosum with palmitic and linoleic acid as dominant compounds [7].However, the composition of fatty acids in these fruits can vary with time.

Conclusions
Extracts of Arctium species fruits contain mainly lignans and fatty acids and the composition is greatly affected by the growing conditions in the year of fruit collection.In the year 2021 with very low total precipitation, more extract per gram of the dried fruit was obtained and it had a greater content of lignans than in 2022, a year with roughly normal total precipitation.There are also differences between Arctium species.A. minus fruits are characterized by a lower content of the major lignan arctiin, a greater content of aglycone arctigenin, and by the presence of linolelaidic acid as compared to the fruits of the most researched species A. lappa.Based on these results, we conclude that Arctium species fruits can serve as sources of bioactive compounds.However, the choice of Arctium species and environmental factors such as the availability of water should be considered when collecting the fruits.

Fig. 1
Fig. 1 PCA analysis of A. lappa (samples obtained in 2021 and 2022, red and orange, respectively), A. minus (samples obtained in 2021 and 2022, blue and turquoise, respectively), and A. tomentosum (samples obtained in 2021 and 2022, dark grey and light grey, respectively).Parts A and B show plotting of components 1 and 2 and components 2 and 4, respectively