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Epigallocatechin Gallate (EGCG) Decorating Soybean Seed Ferritin as a Rutin Nanocarrier with Prolonged Release Property in the Gastrointestinal Tract

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Abstract

The instability and low bioavailability of polyphenols limit their applications in food industries. In this study, epigallocatechin gallate (EGCG) and soybean seed ferritin deprived of iron (apoSSF) were fabricated as a combined double shell material to encapsulate rutin flavonoid molecules. Firstly, due to the reversible assembly characteristics of phytoferritin, rutin was successfully encapsulated within apoSSF to form a ferritin-rutin complex (FR) with an average molar ratio of 28.2: 1 (rutin/ferritin). The encapsulation efficiency and loading capacity of rutin were 18.80 and 2.98 %, respectively. EGCG was then bound to FR to form FR-EGCG composites (FRE), and the binding number of EGCG was 27.30 ± 0.68 with a binding constant K of (2.65 ± 0.11) × 104 M−1. Furthermore, FRE exhibited improved rutin stability, and displayed prolonged release of rutin in simulated gastrointestinal tract fluid, which may be attributed to the external attachment of EGCG to the ferritin cage potentially reducing enzymolysis in GI fluid. In summary, this work demonstrates a novel nanocarrier for stabilization and sustained release of bioactive polyphenols.

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Abbreviations

DLS:

Dynamic light scattering

EGCG:

Epigallocatechin gallate

FR:

Ferritin-rutin complex

FRE:

FR-EGCG composites

GI:

Gastrointestinal tract

SSF:

Soybean seed ferritin

References

  1. Jakobek L (2015) Interactions of polyphenols with carbohydrates, lipids and proteins. Food Chem 175:556–567

    Article  CAS  Google Scholar 

  2. Koda T, Kuroda Y, Imai H (2008) Protective effect of rutin against spatial memory impairment induced by trimethyltin in rats. Nutr Res 28:629–634

    Article  CAS  Google Scholar 

  3. Gené RM, Cartana C, Adzet T, Marin E, Panella T, Canigueral S (1996) Anti-inflammatory and analgesic activity of Baccharis trimera: identification of its active constituents. Planta Med 62:232–235

  4. Mauludin R, Müller RH, Keck C (2009) Kinetic solubility and dissolution velocity of rutin nanocrystals. Eur J Pharm Sci 36:502–510

    Article  CAS  Google Scholar 

  5. Li Z, Jiang H, Xu C, Gu L (2015) A review: using nanoparticles to enhance absorption and bioavailability of phenolic phytochemicals. Food Hydrocoll 43:153–164

    Article  CAS  Google Scholar 

  6. Ha HK, Kim JW, Lee MR, Lee WJ (2013) Formation and characterization of quercetin-loaded chitosan oligosaccharide/β-lactoglobulin nanoparticle. Food Res Int 52:82–90

    Article  CAS  Google Scholar 

  7. Chen L, Remondetto GE, Subirade M (2006) Food protein-based materials as nutraceutical delivery systems. Trends Food Sci Technol 17:272–283

    Article  CAS  Google Scholar 

  8. Theil EC (2003) Ferritin: at the crossroads of iron and oxygen metabolism. J Nutr 133:1549S–1553S

    CAS  Google Scholar 

  9. Arosio P, Levi S (2002) Ferritin, iron homeostasis, and oxidative damage. Free Radic Biol Med 33:457–463

    Article  CAS  Google Scholar 

  10. Theil EC (2004) Iron, ferritin, and nutrition. Annu Rev Nutr 24:327–343

    Article  CAS  Google Scholar 

  11. Kim M, Rho Y, Jin KS, Ahn B, Jung S, Kim H (2011) pH-dependent structures of ferritin and apoferritin in solution: disassembly and reassembly. Biomacromolecules 12:1629–1640

    Article  CAS  Google Scholar 

  12. Yang R, Chen L, Yang S, Lv C, Leng X, Zhao G (2014) 2D square arrays of protein nanocages through channel-directed electrostatic interaction with poly(α, L-lysine). Chem Commun 50:2879–2882

    Article  CAS  Google Scholar 

  13. Lee PS, Yim SG, Choi Y, Ha TVA, Ko S (2012) Physiochemical properties and prolonged release behaviours of chitosan-denatured β-lactoglobulin microcapsules for potential food applications. Food Chem 134:992–998

    Article  CAS  Google Scholar 

  14. Zhao G (2010) Phytoferritin and its implications for human health and nutrition. BBA- GEN SUBJECTS 1800:815–823

    Article  CAS  Google Scholar 

  15. Yang R, Zhou Z, Sun G, Gao Y, Xu J (2015) Ferritin, a novel vehicle for iron supplementation and food nutritional factors encapsulation. Trends Food Sci Technol 44:189–200

    Article  CAS  Google Scholar 

  16. Gómez-Mascaraque LG, Lagarón JM, López-Rubio A (2015) Electrosprayed gelatin submicroparticles as edible carriers for the encapsulation of polyphenols of interest in functional foods. Food Hydrocoll 49:42–52

    Article  Google Scholar 

  17. Wei Z, Yang W, Fan R, Yuan F, Gao Y (2015) Evaluation of structural and functional properties of protein-EGCG complexes and their ability of stabilizing a model b-carotene emulsion. Food Hydrocoll 45:337–350

    Article  CAS  Google Scholar 

  18. Shpigelman A, Israeli G, Livney YD (2010) Thermally-induced protein-polyphenol co-assemblies: beta lactoglobulin-based nanocomplexes as protective nanovehicles for EGCG. Food Hydrocoll 24:735–743

    Article  CAS  Google Scholar 

  19. Petzke KJ, Schuppe S, Rohn S, Rawel HM, Kroll J (2005) Chlorogenic acid moderately decreases the quality of whey proteins in rats. J Agric Food Chem 53:3714–3720

    Article  CAS  Google Scholar 

  20. Wang A, Kai Z, Xin Q, Zhao G (2014) Phytoferritin association induced by EGCG inhibits protein degradation by proteases. Plant Foods Hum Nutr 69:386–391

    Article  CAS  Google Scholar 

  21. Deng J, Li M, Zhang T, Chen B, Leng X, Zhao G (2011) Binding of proanthocyanidins to soybean (Glycine max) seed ferritin inhibiting protein degradation by protease in vitro. Food Res Int 44:33–38

  22. Bauminger ER, Harrison PM, Hechel D, Nowik I, Treffry A (1991) Iron (iii) can be transferred between ferritin molecules. Proc Biol Sci Sci 244:211–217

  23. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  Google Scholar 

  24. Yang R, Zhou Z, Sun G, Gao Y, Xu J, Strappe P, Chris B, Chen Y, Ding X (2015) Synthesis of homogeneous protein-stabilized rutin nanodispersions by reversible assembly of soybean (Glycine max) seed ferritin. RSC Adv 5:31533–31540

  25. Bou-Abdallah F, Zhao G, Biasiotto G, Poli M, Arosio P, Chasteen ND (2008) Facilitated diffusion of iron(II) and dioxygen substrates into human H-chain ferritin. A fluorescence and absorbance study employing the ferroxidase center substitution Y34W. J Am Chem Soc 130:17801–17811

    Article  CAS  Google Scholar 

  26. Lv C, Bai Y, Yang S, Zhao G, Chen B (2013) NADH induces iron release from pea seed ferritin: a model for interaction between coenzyme and protein components in foodstuffs. Food Chem 141:3851–3858

    Article  CAS  Google Scholar 

  27. Işık N, Alteheld B, Kühn S, Schulze-Kaysers N, Kunz B, Wollseifen HR (2014) Polyphenol release from protein and polysaccharide embedded plant extracts during in vitro digestion. Food Res Int 65:109–114

  28. Yang R, Chen L, Zhang T, Yang S, Leng X, Zhao G (2014) Self-assembly of ferritin nanocages into linear chains induced by poly(α, L-lysine). Chem Commun 50:481–483

    Article  CAS  Google Scholar 

  29. Meldrum FC, Wade WJ, Nimmo DL, Heywood BR, Mann S (1991) Synthesis of inorganic nanophase materials in supramolecular protein cages. Nature 349:684–687

    Article  CAS  Google Scholar 

  30. Xue J, Tan C, Zhang X, Feng B, Xia S (2014) Fabrication of epigallocatechin-3-gallate nanocarrier based on glycosylated casein: stability and interaction mechanism. J Agric Food Chem 62:4677–4684

    Article  CAS  Google Scholar 

  31. Cheynier V, Rigaud J, Souquet JM, Duprat F, Moutounet M (1990) Must browning in relation to the behavior of phenolic compounds during oxidation. Am J Enol Vitic 41:346–349

    CAS  Google Scholar 

  32. Liazid A, Palma M, Brigui J, Barroso CG (2007) Investigation on phenolic compounds stability during microwave-assisted extraction. J Chromatogr A 1140:29–34

    Article  CAS  Google Scholar 

  33. Kerdudo A, Dingas A, Fernandez X, Faure C (2014) Encapsulation of rutin and naringenin in multilamellar vesicles for optimum antioxidant activity. Food Chem 159:12–19

    Article  CAS  Google Scholar 

  34. Stefanini S, Cavallo S, Wang CQ, Tataseo P, Vecchini P, Giartosio A, Chiancone E (1996) Thermal stability of horse spleen apoferritin and human recombinant H apoferritin. Arch Biochem Biophys 325:58–64

    Article  CAS  Google Scholar 

  35. Yuksel Z, Avci E, Erdem YK (2010) Characterization of binding interactions between green tea flavonoids and milk proteins. Food Chem 121:450–456

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was financially supported by National Nature Science Foundation of China (No. 31501489), Nature Science Foundation of Tianjin (youth program) (16JCQNJC14500), Nature Science Foundation of China (No. 31471701), and Tianjin Research Program of Application Foundation and Advanced Technology (15JCZDJC34300).

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Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China

    Rui Yang, Guoyu Sun, Min Zhang & Zhongkai Zhou

  2. School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Rui Yang & Zhongkai Zhou

  3. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 10083, China

    Quanhong Li

  4. ARC Industrial Transformation Training Centre for Functional Grains, Wagga Wagga, NSW, 2678, Australia

    Padraig Strappe & Chris Blanchard

  5. School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2678, Australia

    Padraig Strappe & Chris Blanchard

Authors
  1. Rui Yang
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  2. Guoyu Sun
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  3. Min Zhang
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  4. Zhongkai Zhou
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  7. Chris Blanchard
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Correspondence to Rui Yang or Zhongkai Zhou.

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Yang, R., Sun, G., Zhang, M. et al. Epigallocatechin Gallate (EGCG) Decorating Soybean Seed Ferritin as a Rutin Nanocarrier with Prolonged Release Property in the Gastrointestinal Tract. Plant Foods Hum Nutr 71, 277–285 (2016). https://doi.org/10.1007/s11130-016-0557-2

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  • DOI: https://doi.org/10.1007/s11130-016-0557-2

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