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Effect of Allium flavum L. and Allium melanantherum Panč. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase

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Abstract

Allium flavum L. and Allium melanantherum Panč. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.

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Abbreviations

CAT:

catalase

ROS:

reactive oxygen species

SOD:

superoxide dismutase

t-BOOH:

t-butyl hydroperoxide

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Acknowledgments

This work was supported by the Ministry of Science of Republic of Serbia, Project No. 172058.

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Authors and Affiliations

  1. Faculty of Biology, University of Belgrade, Studentski trg 16, Belgrade, 11000, Serbia

    Dragana Mitić-Ćulafić, Biljana Nikolić, Nebojša Jasnić & Jelena Knežević-Vukčević

  2. Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad, Trg Dositeja Obradovica 3, Novi Sad, 21000, Serbia

    Nataša Simin

  3. Faculty of Medicine, Oncology Institute of Vojvodina, University of Novi Sad, Dr Goldmana 4, Sremska Kamenica, 21204, Serbia

    Dragana Četojević-Simin

  4. Faculty of Chemistry, University of Belgrade, Studentski trg 16, Belgrade, 11000, Serbia

    Maja Krstić

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  1. Dragana Mitić-Ćulafić
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  2. Biljana Nikolić
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Correspondence to Dragana Mitić-Ćulafić.

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Mitić-Ćulafić, D., Nikolić, B., Simin, N. et al. Effect of Allium flavum L. and Allium melanantherum Panč. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase. Plant Foods Hum Nutr 71, 28–34 (2016). https://doi.org/10.1007/s11130-015-0519-0

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