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A Non-digestible Fraction of the Common Bean (Phaseolus vulgaris L.) Induces Cell Cycle Arrest and Apoptosis During Early Carcinogenesis

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Abstract

We have previously demonstrated that the non-digestible fraction (NDF) from common cooked beans (P. vulgaris L., cv Negro 8025) inhibits azoxymethane (AOM)-induced colon cancer and influences the expression of genes involved in the induction of apoptosis and cell cycle arrest through the action of butyrate. The objective of this study was to identify cell cycle alterations and morphological changes induced by treatment with AOM and to examine the formation of colonic aberrant crypt foci (ACF) in male Sprague Dawley rats fed with these beans. Rats were fed control diets upon arrival and were randomly placed into four groups after one week of acclimatization: control, NDF (intragastric administration), NDF + AOM and AOM. Rats treated with NDF + AOM exhibited a significantly lower number of total colonic ACF with a notable increase in the number of cells present in the G1 phase (83.14 %); a decreased proliferation index was observed in the NDF + AOM group when compared to AOM group. NDF + AOM also displayed a higher number of apoptotic cells compared to AOM group. NDF of cooked common beans inhibited colon carcinogenesis at an early stage by inducing cell cycle arrest of colon cells and morphological changes linked to apoptosis, thus confirming previous results obtained with gene expression studies.

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Abbreviations

CRC:

Colorectal cancer

SCFAs:

Short-chain fatty acids

AOM:

Azoxymethane

ACF:

Aberrant crypt foci

NDF:

Non-digestible fraction

H&E:

Hematoxylin and eosin

TUNEL:

Terminal deoxynucleotidyl transferase nick end-labeling

ANOVA:

One way variance analysis

PE:

Polysaccharide extract

B. lactis :

Bifidobacterium lactis

G1 :

Growth 1 (cell cycle)

S:

Synthesis (cell cycle)

G2 :

Growth 2 (cell cycle)

M:

Mitosis (cell cycle)

G0 :

Resting (cell cycle)

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Acknowledgements

This work was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) grant No. 57536 and the Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP, Celaya).

Conflicts of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Facultad de Ingeniería, Universidad Autónoma de Querétaro, Queretaro, CP. 76010, Mexico

    Ana Angelica Feregrino-Perez & Ramón G. Guevara-González

  2. Departamento Unidad Docente, Hospital Arnau de Vilanova, Lleida, España C.P. 25198, Mexico

    Carmen Piñol-Felis & Xavier Gomez-Arbones

  3. PROPAC; Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, CP 76010, Mexico

    Rocio Campos-Vega & Guadalupe Loarca-Piña

  4. INIFAP, National Research Institute for Forestry, Agriculture and Livestock, Region Centro, Campus Bajio, Celaya, Gto, Mexico

    Jorge Acosta-Gallegos

Authors
  1. Ana Angelica Feregrino-Perez
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  2. Carmen Piñol-Felis
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  3. Xavier Gomez-Arbones
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  4. Ramón G. Guevara-González
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  5. Rocio Campos-Vega
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  6. Jorge Acosta-Gallegos
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  7. Guadalupe Loarca-Piña
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Corresponding author

Correspondence to Guadalupe Loarca-Piña.

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Feregrino-Perez, A.A., Piñol-Felis, C., Gomez-Arbones, X. et al. A Non-digestible Fraction of the Common Bean (Phaseolus vulgaris L.) Induces Cell Cycle Arrest and Apoptosis During Early Carcinogenesis. Plant Foods Hum Nutr 69, 248–254 (2014). https://doi.org/10.1007/s11130-014-0428-7

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  • DOI: https://doi.org/10.1007/s11130-014-0428-7

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