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Reduced-calorie Avocado Paste Attenuates Metabolic Factors Associated with a Hypercholesterolemic-high Fructose Diet in Rats

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Abstract

The objective of this study was to evaluate the effect of reduced-calorie avocado paste on lipid serum profile, insulin sensitivity, and hepatic steatosis in rats fed a hypercholesterolemic-high fructose diet. Thirty five male Wistar rats were randomly separated in five groups: Control group (ground commercial diet); hypercholesterolemic diet plus 60 % fructose solution (HHF group); hypercholesterolemic diet plus 60 % fructose solution supplemented with avocado pulp (HHF+A group); hypercholesterolemic diet plus 60 % fructose solution supplemented with reduced-calorie avocado paste (HHF+P group); and hypercholesterolemic diet plus 60 % fructose solution supplemented with a reduced-calorie avocado paste plus fiber (HHF+FP group). The A, P, and FP were supplemented at 2 g/kg/d. The study was carried out for seven weeks. Rats belonging to the HHF group exhibited significantly (P ≤ 0.05) higher total cholesterol, triglycerides, and insulin levels in serum as well as lower insulin sensitivity than the control group. Supplementation with reduced-calorie avocado paste showed a significant (P ≤ 0.05) decrease in total cholesterol (43.1 %), low-density lipoprotein (45.4 %), and triglycerides (32.8 %) in plasma as well as elevated insulin sensitivity compared to the HHF group. Additionally, the liver enzymes alanine aminotransferase and aspartate aminotransferase decreased significantly in the HHF-P group (39.8 and 35.1 %, respectively). These results are likely due to biocompounds present in the reduced-calorie avocado paste, such as polyphenols, carotenoids, chlorophylls, and dietary fibre, which are capable of reducing oxidative stress. Therefore, reduced-calorie avocado paste attenuates the effects of a hypercholesterolemic-high fructose diet in rats.

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Abbreviations

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

HDL-C:

High-density lipoprotein cholesterol

HOMA-IR:

Homeostasis model assessment-insulin resistance

IR:

Insulin resistance

LDL-C:

Low-density lipoprotein cholesterol

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Acknowledgments

This work was supported by the Instituto Politécnico Nacional and CONACyT scholarship 176073. The authors gratefully acknowledge Lucia Quevedo-Corona of the Departmento de Fisiología-IPN for technical support.

Conflict of Interest

All authors declared that there is no conflict of interest.

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Authors and Affiliations

  1. Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala s/n Col. Casco de Santo Tomás, México, D.F, 11340, México

    María Elena Pahua-Ramos, Lidia Dorantes-Alvarez & Alicia Ortiz-Moreno

  2. Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n Col. Unidad Profesional Adolfo López Mateos, México, D.F, 07738, México

    Leticia Garduño-Siciliano & German Chamorro-Cevallos

  3. Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala s/n, Col. Casco de Santo Tomás, México, D.F, 11340, México

    Julieta Herrera-Martínez

  4. Universidad de Chalcatongo, Avenida Universidad s/n, Chalcatongo de Hidalgo, Oaxaca, 71100, México

    Obed Osorio-Esquivel

Authors
  1. María Elena Pahua-Ramos
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  2. Leticia Garduño-Siciliano
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  3. Lidia Dorantes-Alvarez
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  4. German Chamorro-Cevallos
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  5. Julieta Herrera-Martínez
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  6. Obed Osorio-Esquivel
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  7. Alicia Ortiz-Moreno
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Correspondence to Alicia Ortiz-Moreno.

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Pahua-Ramos, M.E., Garduño-Siciliano, L., Dorantes-Alvarez, L. et al. Reduced-calorie Avocado Paste Attenuates Metabolic Factors Associated with a Hypercholesterolemic-high Fructose Diet in Rats. Plant Foods Hum Nutr 69, 18–24 (2014). https://doi.org/10.1007/s11130-013-0395-4

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  • DOI: https://doi.org/10.1007/s11130-013-0395-4

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