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Effect of different light intensity on physiology, antioxidant capacity and photosynthetic characteristics on wheat seedlings under high CO2 concentration in a closed artificial ecosystem

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Abstract

The growth of plants under high carbon dioxide (CO2) concentrations (≥ 1000 ppm) is explored for the climate change and the bioregenerative life support system (BLSS) environment of long-duration space missions. Wheat (Triticum aestivum L.) is a grass cultivated for cereal grain—a global staple food including astronauts. Light and CO2 are both indispensable conditions for wheat seedlings. This study provides insights on the physiology, antioxidant capacity and photosynthetic characteristics of wheat seedlings under a range of photosynthetic photon flux densities in a closed system simulating BLSS with high CO2 concentration. We found that the Fv/Fm, Fv/F0, chlorophyll content, intrinsic water use efficiencies (WUEi), membrane stability index (MSI), and malondialdehyde (MDA) of wheat seedlings grown under an intermediate light intensity of 600 μmol m−2 s−1 environment were all largest. Interestingly, the high light intensity of 1200 mol m−2 s−1 treatment group exhibits the highest net photosynthetic rate but the lowest MDA content. The stomatal conductance and F0 of high light intensity of 1000 μmol m−2 s−1 treatment group were both significantly higher than that of other groups. Our study provides basic knowledge on the wheat growth in different environments, especially in a closed ecosystem with artificial lights.

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Abbreviations

HL:

High light

IL:

Intermediate light

LL:

Low light

PPFD:

Photosynthetic photon flux density

LED:

Light-emitting diodes

LP1:

Lunar palace 1

BLSS:

Bioregenerative life support system

MSI:

Membrane stability index

RWC:

Relative water content

POD:

Peroxidase

MDA:

Malondialdehyde

A :

Net photosynthetic rate

gs:

Stomatal conductance

WUEi :

Intrinsic water use efficiencies

Chl a:

Chlorophyll a

Chl b:

Chlorophyll b

Chl a + b:

Total chlorophyll content

PS II:

Photosystem II

F 0 :

Minimum fluorescence

F m :

Maximum fluorescence

F v :

Variable fluorescence

Fv/Fm :

Maximum quantum yield of PSII efficiency

Fv/F0 :

Potential activity of PS II

H2O2 :

Hydrogen peroxide

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (PR China, No. 31870852), and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China

    Zhihao Yi, Jingjing Cui, Yuming Fu & Hong Liu

  2. Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, 100083, China

    Yuming Fu & Hong Liu

  3. Institute of Environmental Biology and Life Support Technology, Beihang University, Beijing, 100191, China

    Zhihao Yi, Jingjing Cui, Yuming Fu & Hong Liu

  4. International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing, 100191, China

    Zhihao Yi, Jingjing Cui, Yuming Fu & Hong Liu

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  1. Zhihao Yi
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Yi, Z., Cui, J., Fu, Y. et al. Effect of different light intensity on physiology, antioxidant capacity and photosynthetic characteristics on wheat seedlings under high CO2 concentration in a closed artificial ecosystem. Photosynth Res 144, 23–34 (2020). https://doi.org/10.1007/s11120-020-00726-x

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